A Novel Divergent Geminivirus Identified in Asymptomatic New World Cactaceae Plants

"Cactaceae comprise a diverse and iconic group of flowering plants which are almost exclusively indigenous to the New World. The wide variety of growth forms found amongst the cacti have led to the trafficking of many species throughout the world as ornamentals. Despite the evolution and physio...

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Detalles Bibliográficos
Autores: Rafaela Salgado Fontenele, Andrew M Salywon, Lucas Charles Majure, Ilaria N Cobb, Amulya Bhaskara, Jesús Aarón Avalos Calleros, Gerardo Rafael Argüello Astorga, Kara Schmidlin, Anthony Khalifeh, Kendal Smith, Joshua Schreck, Michael C. Lund, Matias Köhler, Martin Wojciechowski, Wendy C. Hodgson, Raúl Puente Martínez, koenraad van doorslaer, Safaa G. Kumari, Christian Vernière, Denis Filloux, Philippe Roumagnac, Pierre F Lefeuvre, Simone Ribeiro, Simona Kraberger, Martin Darren, Arvind Varsani
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:México
Institución:Instituto Potosino de Investigación Científica y Tecnológica
Repositorio:Repositorio Institucional del IPICYT
OAI Identifier:oai:ipicyt.repositorioinstitucional.mx:1010/2371
Acceso en línea:http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2371
Access Level:acceso abierto
Palabra clave:info:eu-repo/classification/Autor/Geminivirus
info:eu-repo/classification/Autor/Cactoideae
info:eu-repo/classification/Autor/Opuntioideae
info:eu-repo/classification/Autor/ssDNA virus
info:eu-repo/classification/Autor/Cochineal insects
info:eu-repo/classification/cti/2
info:eu-repo/classification/cti/24
info:eu-repo/classification/cti/2420
Descripción
Sumario:"Cactaceae comprise a diverse and iconic group of flowering plants which are almost exclusively indigenous to the New World. The wide variety of growth forms found amongst the cacti have led to the trafficking of many species throughout the world as ornamentals. Despite the evolution and physiological properties of these plants having been extensively studied, little research has focused on cactus-associated viral communities. While only single-stranded RNA viruses had ever been reported in cacti, here we report the discovery of cactus-infecting single-stranded DNA viruses. These viruses all apparently belong to a single divergent species of the family Geminiviridae and have been tentatively named Opuntia virus 1 (OpV1). A total of 79 apparently complete OpV1 genomes were recovered from 31 different cactus plants (belonging to 20 different cactus species from both the Cactoideae and Opuntioideae clades) and from nine cactus-feeding cochineal insects (Dactylopius sp.) sampled in the USA and Mexico. These 79 OpV1 genomes all share > 78.4% nucleotide identity with one another and < 64.9% identity with previously characterized geminiviruses. Collectively, the OpV1 genomes display evidence of frequent recombination, with some genomes displaying up to five recombinant regions. In one case, recombinant regions span ~40% of the genome. We demonstrate that an infectious clone of an OpV1 genome can replicate in Nicotiana benthamiana and Opuntia microdasys. In addition to expanding the inventory of viruses that are known to infect cacti, the OpV1 group is so distantly related to other known geminiviruses that it likely represents a new geminivirus genus. It remains to be determined whether, like its cactus hosts, its geographical distribution spans the globe."

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