Evaluación de los polimorfismos -2578C/A, -1154G/A y -634G/C del factor de crecimiento endotelial vascular (VEGF-A) y su relación con la susceptibilidad a desarrollar artritis reumatoide en la población mestiza mexicana
Rheumatoid arthritis (RA) is a chronic, inflammatory and autoimmune disease of multifactorial etiology that mainly affects women. Various environmental and genetic factors contribute to its development. Some genes that code proteins and that regulate the immune system have been associated with susce...
| Author: | |
|---|---|
| Format: | master thesis |
| Status: | Published version |
| Publication Date: | 2018 |
| Country: | México |
| Institution: | Universidad Autónoma Metropolitana |
| Repository: | Repositorio Institucional de la UAM Iztapalapa |
| Language: | Spanish |
| OAI Identifier: | oai:bindani.izt.uam.mx:c821gj841 |
| Online Access: | https://doi.org/10.24275/uami.c821gj841 |
| Access Level: | Open access |
| Keyword: | info:eu-repo/classification/LEM/Genética info:eu-repo/classification/LEM/Biología experimental info:eu-repo/classification/LEM/Artritis reumatoide info:eu-repo/classification/cti/2 |
| Summary: | Rheumatoid arthritis (RA) is a chronic, inflammatory and autoimmune disease of multifactorial etiology that mainly affects women. Various environmental and genetic factors contribute to its development. Some genes that code proteins and that regulate the immune system have been associated with susceptibility to RA. One of them is the VEGF-A gene, which codes for vascular endothelial growth factor type A and plays an important role in the angiogenic and pro-inflammatory process in RA. In this gene, single-nucleotide polymorphisms (SNPs) reported as risk factors for RA and other human entities such as -1154G/A (rs1570360), - 2578C/A (rs699947) and -634 G/C have been identified. (rs2010963). Our objective was to determine if the -1154 G/A, -2578 C/A and -634G/C confer risk for RA in our population. The study included 364 cases diagnosed with RA and 323 controls with no family history (in three generations) of inflammatory and autoimmune diseases. Both the cases and the controls were recruited at the Hospital Juárez de México. The DNA was obtained from a peripheral blood sample. Genotyping was performed using the 5 'exonuclease technique using TaqMan probes. The Hardy-Weinberg Equilibrium (E-HW) was evaluated through the Finetti program; while the Odds Ratio (OR), 95% confidence interval (CI) and the value of p were estimated using the Epidat program. The distribution of the SNPs -2578 C / A and -1154 G / A between the cases and controls did not present statistically significant differences. However, the C allele of the SNP -634 G / C presented a higher frequency in cases compared to the controls for general population (OR = 1.97, P = 0.003) and women (OR = 1.86, P = 0.011), nevertheless Hardy-Weinberg equilibrium was not met. Our data suggest that the SNP -634 G / C confers risk for RA in our study population. This is the first study to identify an association between the -634 G / C variant of VEGF-A with susceptibility to RA. |
|---|