Extraction and purification of B-phycoerythrin from the red microalga Rhodosorus marinus
A description is given of the purification of B-phycoerythrin (B-PE) from the red microalga Rhodosorus marinus. Initially, phycobiliproteins were released from the microalgal cells by manual cellular fragmentation and sonication. B-PE was extracted with ammonium sulfate precipitation, and purified b...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2009 |
| País: | México |
| Institución: | Universidad de Sonora |
| Repositorio: | Redalyc-USON |
| OAI Identifier: | oai:redalyc.org:48013184004 |
| Acceso en línea: | https://www.redalyc.org/articulo.oa?id=48013184004 |
| Access Level: | acceso abierto |
| Palabra clave: | Ciencias de la Tierra pigments Microalgae phycoerythrin phycobiliproteins Rhodosorus marinus |
| Sumario: | A description is given of the purification of B-phycoerythrin (B-PE) from the red microalga Rhodosorus marinus. Initially, phycobiliproteins were released from the microalgal cells by manual cellular fragmentation and sonication. B-PE was extracted with ammonium sulfate precipitation, and purified by anionic and size exclusion chromatography. Its purity was tested using indexes and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Spectroscopic characterization of B-PE was performed by UV-visible spectroscopy, fluorescence spectroscopy, and circular dichroism. Rhodosorus marinus showed three types of phycobiliproteins: phycoerythrin, phycocyanin, and allophycocyanin. The purified B-PE showed a purity ratio (A545/A280) of 4.8, characteristic peaks at 540 and 562 nm with a shoulder at 498 nm, fluorescence emission maximum at 578 nm, and a secondary structure almost stable with pH changes. B-PE was found to be the predominant pigment in R. marinus and this red microalga could be a viable option for the recovery of this chromoprotein. |
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