Extraction and purification of B-phycoerythrin from the red microalga Rhodosorus marinus
A description is given of the purification of B-phycoerythrin (B-PE) from the red microalga Rhodosorus marinus. Initially, phycobiliproteins were released from the microalgal cells by manual cellular fragmentation and sonication. B-PE was extracted with ammonium sulfate precipitation, and purified b...
| Authors: | , , , , |
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| Format: | article |
| Status: | Published version |
| Publication Date: | 2009 |
| Country: | México |
| Institution: | UNIVERSIDAD AUTÓNOMA DE BAJA CALIFORNIA |
| Repository: | Ciencias Marinas |
| Language: | English |
| OAI Identifier: | oai:cienciasmarinas.com.mx:article/1614 |
| Online Access: | https://www.cienciasmarinas.com.mx/index.php/cmarinas/article/view/1614 |
| Access Level: | Open access |
| Keyword: | microalgae phycobiliproteins phycoerythrin pigments Rhodosorus marinus microalgas ficobiliproteínas ficoeritrina pigmentos |
| Summary: | A description is given of the purification of B-phycoerythrin (B-PE) from the red microalga Rhodosorus marinus. Initially, phycobiliproteins were released from the microalgal cells by manual cellular fragmentation and sonication. B-PE was extracted with ammonium sulfate precipitation, and purified by anionic and size exclusion chromatography. Its purity was tested using indexes and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Spectroscopic characterization of B-PE was performed by UV-visible spectroscopy, fluorescence spectroscopy, and circular dichroism. Rhodosorus marinus showed three types of phycobiliproteins: phycoerythrin, phycocyanin, and allophycocyanin. The purified B-PE showed a purity ratio(A545/A280) of 4.8, characteristic peaks at 540 and 562 nm with a shoulder at 498 nm, fluorescence emission maximum at 578 nm, and a secondary structure almost stable with pH changes. B-PE was found to be the predominant pigment in R. marinus and this red microalga could be a viable option for the recovery of this chromoprotein. |
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