Fermentación de pulpa de café con Penicillium commune a nivel laboratorio y semi-piloto
The environmental conditions and the pretreatment of coffee pulp for solid-state Penicillium commune fermentation at semi-pilot scale were studied. The semi-pilot reactor used had a capacity of nearly 26 Kg of wet pulp, and an internal mixing system consisted of two helicoidal ribbons. The environme...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2003 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:t722h930z |
| Acceso en línea: | https://doi.org/10.24275/uami.t722h930z |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Fermentación info:eu-repo/classification/LEM/Café info:eu-repo/classification/LEM/Fermentation info:eu-repo/classification/LEM/Coffee info:eu-repo/classification/cti/6 |
| Sumario: | The environmental conditions and the pretreatment of coffee pulp for solid-state Penicillium commune fermentation at semi-pilot scale were studied. The semi-pilot reactor used had a capacity of nearly 26 Kg of wet pulp, and an internal mixing system consisted of two helicoidal ribbons. The environmental conditions studied were: initial moisture, inoculum size and temperature. The effect of thermal pretreatment of substrate, expressed in terms of received energy per kg of wet pulp was also studied. The CO2 production was the indirect measure of growth and final pH was the indicator of caffeine degradation. The following conditions were tested in semi-pilot reactor: moisture, 62.1-71.3%; inoculum size, 2.9 x106 -9.2 x106 spores/g initial wet pulp; and initial temperature, 30-33 ºC. The coffee pulp received 5519-9597 KJ/Kg wet pulp as thermal treatment. Under these conditions the fermentations showed preferential growth of natural microflora (mainly Aspergillus) over Penicillium growth. Therefore, it was decided to study the environmental conditions at laboratory scale (fermentation columns of 100 g wet pulp). The chosen values are: initial moisture 65%. inoculum size, 1x107 spores/g initial wet pulp and initial temperature 25 ºC. The results suggest that thermal treatment must not exceed 1272 KJ/Kg wet pulp in order to favor the Penicillium strain growth. A germination optimum temperature equal to 25 ºC and a mycelium growth optimum temperature of 33.6 ºC were estimated. In semipilot scale, the selection of appropriate environmental conditions resulted in fermenta tions without Aspergillus growth. The alkaline final pH observed suggests caffeine degradation. The results of static fermentation suggest better growth at semi-pilot level than obtained at laboratory level. The solid mixing after germination reduces the bed temperature and the production of CO2. |
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