Actividad y glicosilación de las colinesterasas en ratones con hígado graso no alcohólico
The liver may contain fat and maintain their functionality; various injuries can also increase fat infiltration leading to fatty liver diseases of non-alcoholic origin (NAFLD). Fat liver accumulation can be induced by a hypercholesterolemic diet, cholesterol is important because it promotes awarenes...
| Autor: | |
|---|---|
| Formato: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | México |
| Recursos: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:8910jv059 |
| Acesso em linha: | https://doi.org/10.24275/uami.8910jv059 |
| Access Level: | acceso abierto |
| Palavra-chave: | info:eu-repo/classification/LEM/Fatty liver info:eu-repo/classification/LEM/Glicosilación info:eu-repo/classification/LEM/Hígado graso info:eu-repo/classification/LEM/Glycosylation info:eu-repo/classification/cti/3 |
| Resumo: | The liver may contain fat and maintain their functionality; various injuries can also increase fat infiltration leading to fatty liver diseases of non-alcoholic origin (NAFLD). Fat liver accumulation can be induced by a hypercholesterolemic diet, cholesterol is important because it promotes awareness of cytotoxic stimuli. In mammals, the liver has the ability to synthesize cholinesterases (ChEs) and is the source of butyrylcholinesterase circulating in the plasma. The ChEs are glycoproteins having multiple sites for N-glicosylation. The addition and / or rearrangement of sugars is an important process in the three dimensional structure and functional capabilities of ChEs Objective The purpose of this study was to estimate the acetyl- and butyrylcholinesterase activities and its glycosylation in non-alcoholic fatty liver disease in mice Results A high cholesterol diet cause an increase in cholesterol and a decrease in plasma triacylglycerol content Specific activity of acetylcholinesterase is higher in hypercholesterolemic (HC) mice compared to control mice livers Specific activity of butyrylcholinesterase in hypercholesterolemic mice is significantly lower compared to values estimated in control mice livers. Glycosylation profile from liver cholinesterases with different lectins specificity is modified by hypercholesterolemic diet, and did not show a time-dependent behavior. Conclusion Fat liver infiltration causes an alteration in the activity and/or structure of both cholinesterases, being AChE the most altered. The structural-functional alteration could be associated to abnormal post-translational processing. For BChE, activity level decreases suggest an association with metabolic syndrome, diabetes, hypertension, hyperlipidemia and/or hepatic steatosis. |
|---|