Efecto del sulfonato de perfluorooctano PFOS en las uniones comunicantes de los COCs porcinos durante la maduración in vitro (MIV)
Perfluorooctane sulfonate (PFOS) is a persistent and bio-accumulative pollutant ubiquitous in wildlife and humans, which receives many concerns on its fate, transport, distribution, and toxicity. There is a concern about its presence in human blood and internal organs which points toward association...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2014 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:m326m1871 |
| Acceso en línea: | https://doi.org/10.24275/uami.m326m1871 |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Infertiliadad en animales info:eu-repo/classification/LEM/Sulfonato de perfluorooctano info:eu-repo/classification/LEM/Infertility in animals info:eu-repo/classification/LEM/Toxicology info:eu-repo/classification/LEM/Toxicología reproductiva info:eu-repo/classification/LEM/Swine -- Reproduction info:eu-repo/classification/LEM/Cerdos info:eu-repo/classification/LEM/Perfluorooctane sulfonate info:eu-repo/classification/cti/6 |
| Sumario: | Perfluorooctane sulfonate (PFOS) is a persistent and bio-accumulative pollutant ubiquitous in wildlife and humans, which receives many concerns on its fate, transport, distribution, and toxicity. There is a concern about its presence in human blood and internal organs which points toward associations with multiple human health endpoints including fertility. Researches have shown that PFOS inhibits Gap Junction Communications (GJC), and as levels of PFCs are found in human follicular fluid it might have a deleterious effect on fertilization rates. The aim of this study was to determine whether PFOS has an effect on GJC during oocyte maturation using as a model in vitro matured porcine oocytes. In order to get information about the molecular mechanisms of injury by PFOS, its effect on the RNA expression of Cx45, Cx43 and Cx60 was analysed at 4.5h, 8.5h and 44h in oocytes matured in vitro. The Lethal Dose50 (LD50) for oocytes was 29 μM, and the Inhibition of In vitro Maturation50 (IVM50) was 23 μM, thus indicating that the population occupationally exposed to PFOS is at risk since this concentration is very similar to that found in the plasma of plant workers. When COCs were matured at the IVM50, it was observed that PFOS inhibits Oocyte calcein uptake through gap junctions; In addition, the Meiotic Maturation of oocytes was inhibited. However, PFOS did not alter Cx45, Cx43, and Cx60 RNA expression, which apparently indicate that it does not affect at the genomic level. These findings indicate the need to check the interference of PFOS in the meiotic maturation pathways, and also revise the connexin post-translational modifications, since these are crucial in the signalling to start the nuclear maturation process. |
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