Efecto del sulfonato de perfluorooctano (PFOS) en la vía de señalización de la esteroidogénesis en el ovario murino
Perfluorooctane sulfonate (PFOS) is an aliphatic compound wherein the hydrogen is replaced by fluorine, it is considered a persistent pollutant that causes damage to organisms, and it is used in the manufacture of textiles, personal care and food packaging for daily use. In the serum of humans expos...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:1j92g761x |
| Acceso en línea: | https://doi.org/10.24275/uami.1j92g761x |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Útero info:eu-repo/classification/LEM/Uterus info:eu-repo/classification/LEM/Sulfonato de perfluorooctano info:eu-repo/classification/LEM/Perfluorooctane sulfonate info:eu-repo/classification/LEM/Rats -- Reproduction info:eu-repo/classification/LEM/Ratas info:eu-repo/classification/LEM/Células de la teca info:eu-repo/classification/LEM/Ovarios info:eu-repo/classification/LEM/Ovaries info:eu-repo/classification/LEM/Células de la granulosa info:eu-repo/classification/LEM/Cells info:eu-repo/classification/cti/6 |
| Sumario: | Perfluorooctane sulfonate (PFOS) is an aliphatic compound wherein the hydrogen is replaced by fluorine, it is considered a persistent pollutant that causes damage to organisms, and it is used in the manufacture of textiles, personal care and food packaging for daily use. In the serum of humans exposed and no exposed occupationally has been detected at concentrations 400 a 900 y 3 a 30 ng/ ml respectively. It has been reported that PFOS can be considered as an endocrine disruptor. However, its effect on proteins like EGFR, ERK 1/2 y StAR involved in the signaling pathway of steroidogenesis in granulosa cells (CG) and thecal cell (CT) has not been studied yet. In this study, female mice were administered with doses of 0, 1.4, 10 and 21 µmol /kg of PFOS for 8 days in the 13rd postnatal day. PFOS did not change the body weight of the female mice administered, however it was found an increase in the length of female mice ovaries treated with 10 µmol /kg. Furthermore, it induced a decrease of EGFR in the CG but an increase in CT. p-EGFR was not significantly changed in CT; however, in CG it increased slightly. The PFOS did not cause significant alterations in the expression of ERK 1/2 in CG, but it is observed a tendency of increasing in 1.4 and 10 µmol /kg, while in CT it was observed a decrease in 10 µmol /kg. Moreover, the effect of PFOS caused an increase on p-ERK 1/2 in CG in the dose 10 µmol /kg, while a decrease was observed in CT with same dose. Finally, it was observed that the CT of females treated with PFOS 1.4 and 21 µmol /kg, have a tendency to increase in StAR content while those treated with 10 µmol /kg reduced its content, however no significant differences were found. Further experiments are necessary to evaluate other parameters that help to elucidate the molecular mechanism of PFOS toxicity, some of the experiments proposed are: to evaluate the expression of the mRNA of PPAR, MMPs, ERK 1/2, CREB y SF-1. |
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