Indirect somatic embryogenesis: An efficient and genetically reliable clonal propagation system for Ananas comosus L. Merr. Hybrid “MD2”
The objective of this study was to establish an efficient—direct or indirect—regeneration system for pineapple (Ananas comosus L.) plants, with a high rate of multiplication and that would preserve the genetic identity of the donor genotype (Hybrid ‘MD2’) in the regenerated plants. Ten treatments, w...
| Autores: | , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | México |
| Institución: | Centro de Investigación Científica de Yucatán |
| Repositorio: | Repositorio Institucional CICY |
| Idioma: | inglés |
| OAI Identifier: | oai:cicy.repositorioinstitucional.mx:1003/2818 |
| Acceso en línea: | http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2818 |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/Autores/ANANAS COMOSUS info:eu-repo/classification/Autores/SOMATIC EMBRYOGENESIS info:eu-repo/classification/Autores/MICROPROPAGATION info:eu-repo/classification/Autores/ORGANOGENESIS info:eu-repo/classification/Autores/SSR MARKERS info:eu-repo/classification/Autores/ISSR MARKERS info:eu-repo/classification/Autores/PINEAPPLE info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2415 info:eu-repo/classification/cti/241502 |
| Sumario: | The objective of this study was to establish an efficient—direct or indirect—regeneration system for pineapple (Ananas comosus L.) plants, with a high rate of multiplication and that would preserve the genetic identity of the donor genotype (Hybrid ‘MD2’) in the regenerated plants. Ten treatments, with different concentrations of 2,4-Dichlorophenoxyacetic (2,4-D) and Picloram (P), in the absence or presence of 6-Benzylaminopurine (BAP), were used for in vitro morphogenesis induction, as well as histological and molecular techniques, in order to characterize the morphogenic responses induced. Significant differences between treatments tested, to induce callus and buds, were assessed by the Kruskal Wallis method and the Mann–Whitney U-tests. Different pineapple regeneration routes were identified, showing the high regeneration potential of this species. The medium containing 2 mg L−1 2,4-D and 2 mg L−1 BAP, where indirect somatic embryogenesis occurred, was selected as the most efficient treatment, with an average of 120 somatic embryos per explant, differing significantly from the rest of the treatments. It was also demonstrated that the pineapple plants regenerated in vitro preserved the genetic identity of the donor genotype, which represents a high degree of confidence for the application of indirect somatic embryogenesis for A. comusus clonal propagation. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. |
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