Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer

In this paper the development of a potentiometric biosen-sor based on the use of the enzyme lipase immobilized in a Nafion membrane on a graphite-epoxy transducer is reported. This device has been used to quantify triglycerides in food samples from the aque-ous extracts obtained by emulsion with Ara...

Descripción completa

Detalles Bibliográficos
Autores: Juan Carlos Escamilla-Mejía, José Antonio Rodríguez, Giaan Arturo Álvarez-Romero, Carlos Andrés Galán-Vidal
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:México
Institución:Universidad Autónoma del Estado de Hidalgo
Repositorio:Redalyc-UAEH
OAI Identifier:oai:redalyc.org:47539242004
Acceso en línea:https://www.redalyc.org/articulo.oa?id=47539242004
Access Level:acceso abierto
Palabra clave:Química
Graphite
Triglycerides
Food Analysis
epoxy Composite
Arabic Gum Extraction
id MX_6e4adce2f4cbf8dfd575588f6686a896
oai_identifier_str oai:redalyc.org:47539242004
network_acronym_str MX
network_name_str México
repository_id_str
spelling Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as TransducerJuan Carlos Escamilla-MejíaJosé Antonio RodríguezGiaan Arturo Álvarez-RomeroCarlos Andrés Galán-VidalQuímicaGraphiteTriglyceridesFood Analysisepoxy CompositeArabic Gum ExtractionIn this paper the development of a potentiometric biosen-sor based on the use of the enzyme lipase immobilized in a Nafion membrane on a graphite-epoxy transducer is reported. This device has been used to quantify triglycerides in food samples from the aque-ous extracts obtained by emulsion with Arabic gum. The proposed methodology does not present significant differences with the spec-trophotometric determination used as contrast. This work constitutes the first report of a potentiometric biosensor based on the detection of changes of pH using a graphite-epoxy composite transducer and the first potentiometric triglyceride biosensor which is not based in ISFET transducers. The methodology designed is a simple and inexpensive alternative that minimizes the use of organic solvents without affecting the precision and accuracy of the analysis in complex food samples such as butter, chips and pastries.Sociedad Química de México2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdf1870-249Xhttps://www.redalyc.org/articulo.oa?id=47539242004Journal of the Mexican Chemical Society (México) Num.1 Vol.59reponame:Redalyc-UAEHinstname:Universidad Autónoma del Estado de Hidalgoinstacron:UAEHenhttp://www.redalyc.org/revista.oa?id=475Journal of the Mexican Chemical Societyinfo:eu-repo/semantics/openAccessoai:redalyc.org:475392420042024-08-23T15:57:46Z
dc.title.none.fl_str_mv Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer
title Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer
spellingShingle Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer
Juan Carlos Escamilla-Mejía
Química
Graphite
Triglycerides
Food Analysis
epoxy Composite
Arabic Gum Extraction
title_short Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer
title_full Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer
title_fullStr Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer
title_full_unstemmed Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer
title_sort Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer
dc.creator.none.fl_str_mv Juan Carlos Escamilla-Mejía
José Antonio Rodríguez
Giaan Arturo Álvarez-Romero
Carlos Andrés Galán-Vidal
author Juan Carlos Escamilla-Mejía
author_facet Juan Carlos Escamilla-Mejía
José Antonio Rodríguez
Giaan Arturo Álvarez-Romero
Carlos Andrés Galán-Vidal
author_role author
author2 José Antonio Rodríguez
Giaan Arturo Álvarez-Romero
Carlos Andrés Galán-Vidal
author2_role author
author
author
dc.subject.none.fl_str_mv Química
Graphite
Triglycerides
Food Analysis
epoxy Composite
Arabic Gum Extraction
topic Química
Graphite
Triglycerides
Food Analysis
epoxy Composite
Arabic Gum Extraction
description In this paper the development of a potentiometric biosen-sor based on the use of the enzyme lipase immobilized in a Nafion membrane on a graphite-epoxy transducer is reported. This device has been used to quantify triglycerides in food samples from the aque-ous extracts obtained by emulsion with Arabic gum. The proposed methodology does not present significant differences with the spec-trophotometric determination used as contrast. This work constitutes the first report of a potentiometric biosensor based on the detection of changes of pH using a graphite-epoxy composite transducer and the first potentiometric triglyceride biosensor which is not based in ISFET transducers. The methodology designed is a simple and inexpensive alternative that minimizes the use of organic solvents without affecting the precision and accuracy of the analysis in complex food samples such as butter, chips and pastries.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1870-249X
https://www.redalyc.org/articulo.oa?id=47539242004
identifier_str_mv 1870-249X
url https://www.redalyc.org/articulo.oa?id=47539242004
dc.language.none.fl_str_mv en
language_invalid_str_mv en
dc.relation.none.fl_str_mv http://www.redalyc.org/revista.oa?id=475
dc.rights.none.fl_str_mv Journal of the Mexican Chemical Society
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Journal of the Mexican Chemical Society
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedad Química de México
publisher.none.fl_str_mv Sociedad Química de México
dc.source.none.fl_str_mv Journal of the Mexican Chemical Society (México) Num.1 Vol.59
reponame:Redalyc-UAEH
instname:Universidad Autónoma del Estado de Hidalgo
instacron:UAEH
instname_str Universidad Autónoma del Estado de Hidalgo
instacron_str UAEH
institution UAEH
reponame_str Redalyc-UAEH
collection Redalyc-UAEH
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1858175646914576385
score 15.81155

Similares en LA Referencia