Monoenzymatic Lipase Potentiometric Biosensor for the Food Analysis Based on a pH Sensitive Graphite-epoxy Composite as Transducer
In this paper the development of a potentiometric biosen-sor based on the use of the enzyme lipase immobilized in a Nafion membrane on a graphite-epoxy transducer is reported. This device has been used to quantify triglycerides in food samples from the aque-ous extracts obtained by emulsion with Ara...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | México |
| Institución: | Universidad Autónoma del Estado de Hidalgo |
| Repositorio: | Redalyc-UAEH |
| OAI Identifier: | oai:redalyc.org:47539242004 |
| Acceso en línea: | https://www.redalyc.org/articulo.oa?id=47539242004 |
| Access Level: | acceso abierto |
| Palabra clave: | Química Graphite Triglycerides Food Analysis epoxy Composite Arabic Gum Extraction |
| Sumario: | In this paper the development of a potentiometric biosen-sor based on the use of the enzyme lipase immobilized in a Nafion membrane on a graphite-epoxy transducer is reported. This device has been used to quantify triglycerides in food samples from the aque-ous extracts obtained by emulsion with Arabic gum. The proposed methodology does not present significant differences with the spec-trophotometric determination used as contrast. This work constitutes the first report of a potentiometric biosensor based on the detection of changes of pH using a graphite-epoxy composite transducer and the first potentiometric triglyceride biosensor which is not based in ISFET transducers. The methodology designed is a simple and inexpensive alternative that minimizes the use of organic solvents without affecting the precision and accuracy of the analysis in complex food samples such as butter, chips and pastries. |
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