Evaluación de indicadores de la maduración espermática epididimaria de la rata wistar expuesta a cadmio
Infertility in males is a problem that has increased, mainly due to pathological alterations. However, 34-40% of cases are due to idiopathic infertility, related to exposure to toxic agents in the environment such as cadmium (Cd), a heavy metal derived from anthropogenic activities. Cd is linked to...
| Autor: | |
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:8p58pc95j |
| Acceso en línea: | https://doi.org/10.24275/uami.8p58pc95j |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Cadmium info:eu-repo/classification/LEM/Animal breeding info:eu-repo/classification/LEM/Ratas -- Espermatozoides info:eu-repo/classification/LEM/Reproducción animal info:eu-repo/classification/LEM/Cadmio info:eu-repo/classification/cti/3 |
| Sumario: | Infertility in males is a problem that has increased, mainly due to pathological alterations. However, 34-40% of cases are due to idiopathic infertility, related to exposure to toxic agents in the environment such as cadmium (Cd), a heavy metal derived from anthropogenic activities. Cd is linked to a decrease in sperm quality parameters in ejaculates of humans and rodents, although up to date it is not described if these effects are associated with an alteration in the functionality of the epididymis, in which spermatozoa mature, by means of an orderly series of physiological, morphological and biochemical changes that provide the characteristics of the fertilizing potential. These include: changes in plasma membrane glycosylation, involving carbohydrates such as N-acetylglucosamine, sialic acid, mannose and fucose, important for the recognition and interaction between gametes, and subsequent fertilization of the oocyte, as well as the phosphorylation of tyrosine residues in sperm proteins, which is the molecular basis for the acquisition of sperm motility, together with maintenance of integrity and compaction Of sperm DNA. Thus, the objective of this work was to analyze the effect of Cd on indicators of epididymal sperm maturation in the Wistar rat. We found that the Cd at a dose of 0.25 mg/kg of CdCl2 by IP, from day 01 to 56 of life, caused a decrease in sperm determination parameters (concentration and vitality), and affects the sperm maturation of the rat Wistar, as it was observed changes in the distribution and composition of N-acetylglucosamine and/or sialic acid, mannose and fucose, increased phosphorylation of tyrosine residues in proteins, and percentages of DNA compaction and integrity decreased in spermatozoa of the three regions of the epididymis of subjects with exposure to this metal. Hence, fertility in the male can be affected by Cd, and therefore contribute to the infertility of idiopathic etiology and be the cause of the decline in conception of the couple. |
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