Bacterial biodegradation of diisononyl phthalate: molecular characterization and optimization
"Di-isononyl phthalate (DINP) is one of plasticizers most employed in the production of plastic materials and belongs to the most important environmental contaminants. In this work, a consortium of saline soil bacterial (SSB) capable of degrading DINP is presented. The genera of SSB-consortium...
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| Formato: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | México |
| Recursos: | Instituto Potosino de Investigación Científica y Tecnológica |
| Repositorio: | Repositorio Institucional del IPICYT |
| OAI Identifier: | oai:ipicyt.repositorioinstitucional.mx:1010/2574 |
| Acesso em linha: | http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/2574 |
| Access Level: | acceso abierto |
| Palavra-chave: | info:eu-repo/classification/Autor/Biodegradation info:eu-repo/classification/Autor/Consortium info:eu-repo/classification/Autor/Degradation info:eu-repo/classification/Autor/Endocrine disruptors info:eu-repo/classification/Autor/Plasticizers info:eu-repo/classification/Autor/Phthalates info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2415 |
| Resumo: | "Di-isononyl phthalate (DINP) is one of plasticizers most employed in the production of plastic materials and belongs to the most important environmental contaminants. In this work, a consortium of saline soil bacterial (SSB) capable of degrading DINP is presented. The genera of SSB-consortium were Serratia sp., Methylobacillus sp., Achromobacter sp., Pseudomonas sp., Stenotrophomonas sp., Methyloversatilis sp., Delftia sp. and Brevundimonas sp. Response surface methodology (RSM) study was employed to optimise and evaluate the culture conditions to improve the biodegradation of DINP. The optimal conditions were a pH 7.0, 31°C and an initial DINP concentration of 500 mg L-1, resulting in almost complete biodegradation (99%) in 168 h. DINP degradation followed a first-order kinetic model, and the half-life was 12.76 h. During the biodegradation of DINP, 4 derived-compounds were identified: monoisononyl phthalate, methyl nonyl phthalate, iso-nonanol, and dimethyl phthalate. The metabolite profiling indicated that DINP was degraded through simultaneous pathways of de-esterification and β-oxidation. Results suggest that the SSB-consortium could be useful for efficient biodegradation of the DINP-contaminated environments." |
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