Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos
The effect of resveratrol on the in vitro maturation (IVM) of ovine (Ovis aries) oocytes and the development of handmade cloned embryos was evaluated. The nuclear maturation and reactive oxygen species (ROS) levels in the oocytes, as well as the early development and morphological cloned embryo qual...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Redalyc-UAM |
| OAI Identifier: | oai:redalyc.org:42371698002 |
| Acceso en línea: | https://www.redalyc.org/articulo.oa?id=42371698002 https://www.redalyc.org/journal/423/42371698002/ https://www.redalyc.org/journal/423/42371698002/html/ https://www.redalyc.org/journal/423/42371698002/42371698002.epub https://www.redalyc.org/journal/423/42371698002/movil |
| Access Level: | acceso abierto |
| Palabra clave: | Veterinaria Ovis aries Resveratrol Handmade cloning Embryo development In vitro maturation |
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Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryosJosé Luis Martínez-IbarraEugenia Adriana Espinoza-MendozaRaymundo Rangel-SantosDemetrio Alonso Ambriz-GarciaMaría del Carmen Navarro-MaldonadoVeterinariaOvis ariesResveratrolHandmade cloningEmbryo developmentIn vitro maturationThe effect of resveratrol on the in vitro maturation (IVM) of ovine (Ovis aries) oocytes and the development of handmade cloned embryos was evaluated. The nuclear maturation and reactive oxygen species (ROS) levels in the oocytes, as well as the early development and morphological cloned embryo quality, were evaluated under different resveratrol concentrations (0, 0.5, 2 and 5 µM). After IVM, no significant difference was observed in the maturation rate of oocytes treated with 0.5 µM (81.3 %) and 2 µM (72 %) resveratrol compared to that of the control group (0 µM) (74.2 %), but the rate significantly decreased at 5 µM (56 %) (p < 0.05). When the oocyte ROS levels were determined, no significant differences among the groups were observed (p > 0.05). For cloned embryo development, the embryos obtained from the oocytes treated with 0.5 µM resveratrol showed higher (p < 0.05) compacted morula rates (10.7 %) compared to the embryos obtained from the oocytes treated with 0, 2 and 5 µM (6.2, 0 and 0 %, respectively). Regarding embryo morphological quality, the embryos from the oocytes treated with 0.5 µM resveratrol showed a lower rate of poor quality morulae (4.7 %) in comparison to those treated with 0, 2 and 5 µM (23.8, 23.3 and 33.3 %, respectively) (p < 0.05). In conclusion, resveratrol showed no significant improvement on the IVM or ROS levels in domestic ovine oocytes. However, treatment with 0.5 µM resveratrol during IVM improved embryo quality and promoted morulae compaction of Ovis aries handmade cloned embryos.Universidad Nacional Autónoma de México2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdf0301-5092https://www.redalyc.org/articulo.oa?id=42371698002https://www.redalyc.org/journal/423/42371698002/https://www.redalyc.org/journal/423/42371698002/html/https://www.redalyc.org/journal/423/42371698002/42371698002.epubhttps://www.redalyc.org/journal/423/42371698002/movil10.22201/fmvz.24486760e.2018.4.491Veterinaria México (México) Num.4 Vol.5reponame:Redalyc-UAMinstname:Universidad Autónoma Metropolitanainstacron:UAMenhttp://www.redalyc.org/revista.oa?id=423Veterinaria Méxicoinfo:eu-repo/semantics/openAccessoai:redalyc.org:423716980022025-03-05T19:08:43Z |
| dc.title.none.fl_str_mv |
Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos |
| title |
Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos |
| spellingShingle |
Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos José Luis Martínez-Ibarra Veterinaria Ovis aries Resveratrol Handmade cloning Embryo development In vitro maturation |
| title_short |
Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos |
| title_full |
Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos |
| title_fullStr |
Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos |
| title_full_unstemmed |
Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos |
| title_sort |
Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos |
| dc.creator.none.fl_str_mv |
José Luis Martínez-Ibarra Eugenia Adriana Espinoza-Mendoza Raymundo Rangel-Santos Demetrio Alonso Ambriz-Garcia María del Carmen Navarro-Maldonado |
| author |
José Luis Martínez-Ibarra |
| author_facet |
José Luis Martínez-Ibarra Eugenia Adriana Espinoza-Mendoza Raymundo Rangel-Santos Demetrio Alonso Ambriz-Garcia María del Carmen Navarro-Maldonado |
| author_role |
author |
| author2 |
Eugenia Adriana Espinoza-Mendoza Raymundo Rangel-Santos Demetrio Alonso Ambriz-Garcia María del Carmen Navarro-Maldonado |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
Veterinaria Ovis aries Resveratrol Handmade cloning Embryo development In vitro maturation |
| topic |
Veterinaria Ovis aries Resveratrol Handmade cloning Embryo development In vitro maturation |
| description |
The effect of resveratrol on the in vitro maturation (IVM) of ovine (Ovis aries) oocytes and the development of handmade cloned embryos was evaluated. The nuclear maturation and reactive oxygen species (ROS) levels in the oocytes, as well as the early development and morphological cloned embryo quality, were evaluated under different resveratrol concentrations (0, 0.5, 2 and 5 µM). After IVM, no significant difference was observed in the maturation rate of oocytes treated with 0.5 µM (81.3 %) and 2 µM (72 %) resveratrol compared to that of the control group (0 µM) (74.2 %), but the rate significantly decreased at 5 µM (56 %) (p < 0.05). When the oocyte ROS levels were determined, no significant differences among the groups were observed (p > 0.05). For cloned embryo development, the embryos obtained from the oocytes treated with 0.5 µM resveratrol showed higher (p < 0.05) compacted morula rates (10.7 %) compared to the embryos obtained from the oocytes treated with 0, 2 and 5 µM (6.2, 0 and 0 %, respectively). Regarding embryo morphological quality, the embryos from the oocytes treated with 0.5 µM resveratrol showed a lower rate of poor quality morulae (4.7 %) in comparison to those treated with 0, 2 and 5 µM (23.8, 23.3 and 33.3 %, respectively) (p < 0.05). In conclusion, resveratrol showed no significant improvement on the IVM or ROS levels in domestic ovine oocytes. However, treatment with 0.5 µM resveratrol during IVM improved embryo quality and promoted morulae compaction of Ovis aries handmade cloned embryos. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
0301-5092 https://www.redalyc.org/articulo.oa?id=42371698002 https://www.redalyc.org/journal/423/42371698002/ https://www.redalyc.org/journal/423/42371698002/html/ https://www.redalyc.org/journal/423/42371698002/42371698002.epub https://www.redalyc.org/journal/423/42371698002/movil 10.22201/fmvz.24486760e.2018.4.491 |
| identifier_str_mv |
0301-5092 10.22201/fmvz.24486760e.2018.4.491 |
| url |
https://www.redalyc.org/articulo.oa?id=42371698002 https://www.redalyc.org/journal/423/42371698002/ https://www.redalyc.org/journal/423/42371698002/html/ https://www.redalyc.org/journal/423/42371698002/42371698002.epub https://www.redalyc.org/journal/423/42371698002/movil |
| dc.language.none.fl_str_mv |
en |
| language_invalid_str_mv |
en |
| dc.relation.none.fl_str_mv |
http://www.redalyc.org/revista.oa?id=423 |
| dc.rights.none.fl_str_mv |
Veterinaria México info:eu-repo/semantics/openAccess |
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Veterinaria México |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Autónoma de México |
| publisher.none.fl_str_mv |
Universidad Nacional Autónoma de México |
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Veterinaria México (México) Num.4 Vol.5 reponame:Redalyc-UAM instname:Universidad Autónoma Metropolitana instacron:UAM |
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Universidad Autónoma Metropolitana |
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UAM |
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UAM |
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Redalyc-UAM |
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Redalyc-UAM |
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