Daño a la integridad del ADN y la membrana del espermatozoide de conejo Nueva Zelanda blanco causados por sobrepeso
Overweight and obesity are clinical conditions where an excess in fat tissue can have negative health effects. The increase in the size or fat cells number can result in physical and hormonal changes in the individual; these changes contribute to some problems such as: increased scrotal temperature...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2015 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:6d56zw75n |
| Acceso en línea: | https://doi.org/10.24275/uami.6d56zw75n |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Rabbits -- Reproduction info:eu-repo/classification/LEM/Daños del ADN info:eu-repo/classification/LEM/Obesity info:eu-repo/classification/LEM/Obesidad info:eu-repo/classification/LEM/Esterilidad masculina info:eu-repo/classification/LEM/Espermatozoides info:eu-repo/classification/LEM/Conejos info:eu-repo/classification/LEM/Spermatozoa info:eu-repo/classification/LEM/Infertility, Male info:eu-repo/classification/LEM/DNA damage info:eu-repo/classification/cti/6 |
| Sumario: | Overweight and obesity are clinical conditions where an excess in fat tissue can have negative health effects. The increase in the size or fat cells number can result in physical and hormonal changes in the individual; these changes contribute to some problems such as: increased scrotal temperature and increased production of reactive oxygen species (ROS), which rises with overweight and obesity, which could cause oxidative stress. This contributes to the correlation described between obesity and male infertility, where studies reported a direct correlation between increased body mass and decreased concentration, motility and sperm viability. However, these studies have only been made in patients attending in fertility clinics, causing a population bias and also knowledge of the biochemical mechanisms that cause these changes, have not been reported. So, through an animal model for overweight induction and semen samples obtaining, we propose the methodology that allowed us to determine if there is damage to the membrane, an effect on DNA fragmentation and compaction state in New Zealand White rabbits (NZW) sperm in overweight condition. So we set out to determine damage in the NZB rabbit sperm, caused by overweight. To generate overweight we were given a high-calorie diet (3.2 Mcal / kg) containing vegetable oil, the normal weight group (control group) were maintained with a diet of commercial food (2.8 Mcal / kg). Studies of semen quality parameters performed considering: concentration, viability, motility and sperm morphology. To study the biochemical mechanisms we were used techniques as lipoperoxidation quantification (Malondialdehyde (MDA) by a colorimetric method with thiobarbituric acid), the DNA fragmentation assessment with acridine orange (by flow cytometry) and decompaction of DNA nuclear sperm with aniline blue method (by light microscopy). Treatment with calorie diet produced 24% of overweight in NZB rabbits, relative to normal weight group. Overweight not induce significant changes in semen quality parameters, in lipoperoxidation of the membrane and nuclear fragmentation of NZB rabbit sperm with overweight. However, the decompaction of DNA nuclear sperm was increased, reaching a value of 1.6% in overweight state and 0.63% in control group (p = 0.002) in the NZB rabbit, though the value was low; sustains reported in NZB rabbit, on overweight could affect epididymal sperm maturation process. Finally we conclude that overweight status in NZB rabbits performed with caloric diet and the nuclear DNA decompaction was increased. |
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