Identificación de los Genes cry en Cepas Mexicanas de Bacillus thuringiensis con Potencial Insecticida

This report revealed the crystal morphology and protein composition of strains LBIT-499 and LBIT-504 of B. thuringiensis, native to Guanajuato, Mexico. LBIT-499 showed only bipyramidal crystals, while LBIT-504 showed both bipyramidal and cubical crystals. These crystals were composed by proteins of...

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Detalles Bibliográficos
Autores: Rosales-Reyes, T., Salcedo-Hernández, R., Ibarra, J. E., Barboza-Corona, J. Eleazar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2003
País:México
Institución:UNIVERSIDAD DE GUANAJUATO
Repositorio:Acta Universitaria
Idioma:español
OAI Identifier:oai:www.actauniversitaria.ugto.mx:article/268
Acceso en línea:https://www.actauniversitaria.ugto.mx/index.php/acta/article/view/268
Access Level:acceso abierto
Palabra clave:Bacillus thuringiensis
Cepas nativas
Genes cry
Proteínas Cry
PCR.
native strains
cry genes
Cry proteins
Descripción
Sumario:This report revealed the crystal morphology and protein composition of strains LBIT-499 and LBIT-504 of B. thuringiensis, native to Guanajuato, Mexico. LBIT-499 showed only bipyramidal crystals, while LBIT-504 showed both bipyramidal and cubical crystals. These crystals were composed by proteins of ca. 130 and 60 kDa, respectively. Both strains as well as LBIT-500 and LBIT-544, showed a great variety of cry1 genes, while cry2A and cry2B were identified in LBT-500 and LBIT-544, LBIT-504 showed only the cry2B gene, and no cry2 was detected in LBIT-499.