Patrones de lacasas de Pleurotus ostreatus desarrollado en fermentación sumergida a diferentes pH del medio de cultivo
The effect of pH (3.5, 4.5, 6.5 and 8.5) of the culture medium of Pleurotus ostreatus on laccases activity and zymogram profile (intra-and extra-cellular), as well as on the expression profiles of five laccase genes (lacc 1, 4, 6, 9 and 10) was evaluated. A liquid culture medium containing glucose,...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:sq87bt81x |
| Acceso en línea: | https://doi.org/10.24275/uami.sq87bt81x |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Fermentation info:eu-repo/classification/LEM/Fermentación en sumergido info:eu-repo/classification/LEM/Pleurotus ostreatus info:eu-repo/classification/LEM/Hongos -- Biotecnología info:eu-repo/classification/LEM/Ensymes info:eu-repo/classification/LEM/Fungi Biotechnology info:eu-repo/classification/LEM/Enzimas lacasas info:eu-repo/classification/cti/6 |
| Sumario: | The effect of pH (3.5, 4.5, 6.5 and 8.5) of the culture medium of Pleurotus ostreatus on laccases activity and zymogram profile (intra-and extra-cellular), as well as on the expression profiles of five laccase genes (lacc 1, 4, 6, 9 and 10) was evaluated. A liquid culture medium containing glucose, yeast extract and mineral salts (including copper inductor) was used. Laccase activity was evaluated at different pH (3.5, 4.5, 6.5 and 8.5) using 2,6-dimethoxyphenol as substrate. The maximum extracellular laccase activity was 77550 U/L at pH 4.5 when the fungus was developed at pH mentioned above. In fermentations at pH 3.5 and 6.5, the laccases activities incubated at pH 4.5 and 3.5 were 29490 and 25000 U/L, respectively. Intracellular activity showed the maximum values in fermentation of 4.5 and 6.5 when were incubated at pH 4.5 and 6.5 (4800 and 1741 U/g dry biomass, respectively). In zymograms, fermentation at pH 3.5 showed three isoforms when was incubated at pH 6.5, and 2 isoforms in the others pH evaluated, three isoforms were observed in fermentation at pH 4.5 when was incubated at pH 4.5, 6.5 and 8.5. Four isoforms were observed in the fermentation at pH 6.5 incubated at the same pH, and 3 isoforms in above fermentation when was incubated at pH 4.5 and 8.5. Only one isoform was observed at pH of 8.5 when was incubated at all pH tested. In intracellular extracts were observed three isoforms in fermentations at 3.5 and 4.5 when were incubated at pH 8.5, 6.5 and 8.5 and two isoforms in the fermentation at pH of 6.5 in all pH of incubation. Differences were observed at level and time of expression of 5 genes (lacc 1, 4, 6, 9 and 10) evaluated. lacc 9 was not expressed in any condition. In the fermentation at pH 3.5, lacc 4, 6 and 10 were expressed with greater intensity. In the fermentation at pH 4.5, lacc 6 and 10 were expressed with greater intensity. In the fermentation at pH 6.5, all genes showed approximately the same level of expression. For fermentation at pH 8.5, lacc 1, 4 and 6 showed the higher expression. In this study, was observed that the pH of development of the fungus is a factor that modifies the patterns of expression and production of the laccases. |
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