Metabolismo energético, estado redox y parámetros de las células MCF10A inducidas a senescencia por estrés oxidante
Cellular senescence (SC) is a biological phenomenon that occurs in response to damage and has been linked to different biological and pathological processes. Much of the knowledge of cellular senescence has been described in fibroblasts; however, this is not the only cell type in which senescence oc...
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| Tipo de recurso: | tesis de maestría |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | México |
| Institución: | Universidad Autónoma Metropolitana |
| Repositorio: | Repositorio Institucional de la UAM Iztapalapa |
| Idioma: | español |
| OAI Identifier: | oai:bindani.izt.uam.mx:k0698751r |
| Acceso en línea: | https://doi.org/10.24275/uami.k0698751r |
| Access Level: | acceso abierto |
| Palabra clave: | info:eu-repo/classification/LEM/Cells -- Aging info:eu-repo/classification/LEM/Oxidation-reduction reaction info:eu-repo/classification/LEM/Oxidative stress info:eu-repo/classification/LEM/Estrés oxidativo info:eu-repo/classification/LEM/Reacción de oxidación-reducción info:eu-repo/classification/LEM/Células -- Envejecimiento info:eu-repo/classification/cti/2 |
| Sumario: | Cellular senescence (SC) is a biological phenomenon that occurs in response to damage and has been linked to different biological and pathological processes. Much of the knowledge of cellular senescence has been described in fibroblasts; however, this is not the only cell type in which senescence occurs. The establishment of senescence can occur due to oxidative stress that induces DNA damage. Aspects such as energy metabolism and the redox state of senescent cells are little studied. In this work, senescence was induced in the MCF10A cell line by oxidative stress. The energy metabolism of senescent cells was determined by measuring the rate of oxygen consumption (OCR) and the acidification rate of the extracellular medium (ECAR), as well as, the redox state evaluating the presence of reactive oxygen species (ROS) and the oxidized and reduced glutathione coefficient (GSH/GSSG).Our results determined that senescent MCF10A cells induced to senescence by oxidative stress exhibit an energy metabolism preferentially directed towards glycolysis, and a redox state prooxidant, due to an elevation in GSSG content that coincides with an increase in ROS. |
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