Macroporous click-elastin-like hydrogels for tissue engineering applications

Producción Científica

Detalles Bibliográficos
Autores: Fernández Colino, Alicia, Wolf, Frederic, Keijdener, Hans, Rütten, Stephan, Schmitz Rodea, Thomas, Jockenhoevel, Stefan, Rodríguez Cabello, José Carlos, Mela, Petra
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/33507
Acceso en línea:https://doi.org/10.1016/j.msec.2018.03.013
http://uvadoc.uva.es/handle/10324/33507
Access Level:acceso abierto
Palabra clave:Elastine
Elastina
Click chemistry
Química del clic
id ES_fff65e620f332774eec095ade3bdbde7
oai_identifier_str oai:uvadoc.uva.es:10324/33507
network_acronym_str ES
network_name_str España
repository_id_str
spelling Macroporous click-elastin-like hydrogels for tissue engineering applicationsFernández Colino, AliciaWolf, FredericKeijdener, HansRütten, StephanSchmitz Rodea, ThomasJockenhoevel, StefanRodríguez Cabello, José CarlosMela, PetraElastineElastinaClick chemistryQuímica del clicProducción CientíficaElastin is a key extracellular matrix (ECM) protein that imparts functional elasticity to tissues and therefore an attractive candidate for bioengineering materials. Genetically engineered elastin-like recombinamers (ELRs) maintain inherent properties of the natural elastin (e.g. elastic behavior, bioactivity, low thrombogenicity, inverse temperature transition) while featuring precisely controlled composition, the possibility for biofunctionalization and non-animal origin. Recently the chemical modification of ELRs to enable their crosslinking via a catalyst-free click chemistry reaction, has further widened their applicability for tissue engineering. Despite these outstanding properties, the generation of macroporous click-ELR scaffolds with controlled, interconnected porosity has remained elusive so far. This significantly limits the potential of these materials as the porosity has a crucial role on cell infiltration, proliferation and ECM formation. In this study we propose a strategy to overcome this issue by adapting the salt leaching/gas foaming technique to click-ELRs. As result, macroporous hydrogels with tuned pore size and mechanical properties in the range of many native tissues were reproducibly obtained as demonstrated by rheological measurements and quantitative analysis of fluorescence, scanning electron and two-photon microscopy images. Additionally, the appropriate size and interconnectivity of the pores enabled smooth muscle cells to migrate into the click-ELR scaffolds and deposit extracellular matrix. The macroporous structure together with the elastic performance and bioactive character of ELRs, the specificity and non-toxic character of the catalyst-free click-chemistry reaction, make these scaffolds promising candidates for applications in tissue regeneration. This work expands the potential use of ELRs and click chemistry systems in general in different biomedical fields.Ministerio de Economía, Industria y Competitividad (Projects MAT2013-42473-R, MAT2015-68901-R, MAT2016- 78903-R)Junta de Castilla y León (programa de apoyo a proyectos de investigación - Ref. VA313U14, VA015U16 y PCIN-2015-010)gobierno federal y estatal de Alemania en el marco del Programa de Posición Rotacional i³tm (2014-R4-01) y del Programa START de la Facultad de Medicina de la Universidad de Aachen (proyecto nº 691713),el centro de imágenes del Centro Interdisciplinario de Investigación Clínica (IZKF) de la Facultad de Medicina de la Universidad de AachenElsevier2018info:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1016/j.msec.2018.03.013http://uvadoc.uva.es/handle/10324/33507reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://www.sciencedirect.com/science/article/pii/S0928493117335282info:eu-repo/semantics/openAccessoai:uvadoc.uva.es:10324/335072026-06-13T12:44:47Z
dc.title.none.fl_str_mv Macroporous click-elastin-like hydrogels for tissue engineering applications
title Macroporous click-elastin-like hydrogels for tissue engineering applications
spellingShingle Macroporous click-elastin-like hydrogels for tissue engineering applications
Fernández Colino, Alicia
Elastine
Elastina
Click chemistry
Química del clic
title_short Macroporous click-elastin-like hydrogels for tissue engineering applications
title_full Macroporous click-elastin-like hydrogels for tissue engineering applications
title_fullStr Macroporous click-elastin-like hydrogels for tissue engineering applications
title_full_unstemmed Macroporous click-elastin-like hydrogels for tissue engineering applications
title_sort Macroporous click-elastin-like hydrogels for tissue engineering applications
dc.creator.none.fl_str_mv Fernández Colino, Alicia
Wolf, Frederic
Keijdener, Hans
Rütten, Stephan
Schmitz Rodea, Thomas
Jockenhoevel, Stefan
Rodríguez Cabello, José Carlos
Mela, Petra
author Fernández Colino, Alicia
author_facet Fernández Colino, Alicia
Wolf, Frederic
Keijdener, Hans
Rütten, Stephan
Schmitz Rodea, Thomas
Jockenhoevel, Stefan
Rodríguez Cabello, José Carlos
Mela, Petra
author_role author
author2 Wolf, Frederic
Keijdener, Hans
Rütten, Stephan
Schmitz Rodea, Thomas
Jockenhoevel, Stefan
Rodríguez Cabello, José Carlos
Mela, Petra
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Elastine
Elastina
Click chemistry
Química del clic
topic Elastine
Elastina
Click chemistry
Química del clic
description Producción Científica
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.msec.2018.03.013
http://uvadoc.uva.es/handle/10324/33507
url https://doi.org/10.1016/j.msec.2018.03.013
http://uvadoc.uva.es/handle/10324/33507
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0928493117335282
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425821739909120
score 15,300719

Similares en LA Referencia