WIP and WASP play complementary roles in T cell homing and chemotaxis to SDF-1a

Producción Científica

Detalhes bibliográficos
Autores: Gallego, María Dolores, Fuente García, Miguel Ángel de la, Antón, Inés María, Snapper, Scott B., Fuhlbrigge, Robert, Geha, Raif S.
Formato: artículo
Fecha de publicación:2005
País:España
Recursos:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/9838
Acesso em linha:https://doi.org/10.1093/intimm/dxh310
http://uvadoc.uva.es/handle/10324/9838
Access Level:acceso abierto
Palavra-chave:Biología celular
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spelling WIP and WASP play complementary roles in T cell homing and chemotaxis to SDF-1aGallego, María DoloresFuente García, Miguel Ángel de laAntón, Inés MaríaSnapper, Scott B.Fuhlbrigge, RobertGeha, Raif S.Biología celularProducción CientíficaHoming of lymphocytes to tissues is a biologically important multistep process that involves selectindependent rolling, integrin-dependent adhesion and chemokine-directed chemotaxis. The actin cytoskeleton plays a central role in lymphocyte adhesion and motility. Wiskott–Aldrich syndrome protein (WASP), the product of the gene mutated in Wiskott–Aldrich syndrome, and its partner, the Wiskott–Aldrich syndrome protein-interacting protein (WIP), play important roles in actin re-organization in T lymphocytes. We used mice with disruption of the WASP and WIP genes to examine the role of WASP and WIP in T cell homing. T cell homing to spleen and lymph nodes in vivo was deficient in WASP / and WIP / mice and severely impaired in WASP / WIP / double knockout (DKO) mice. Deficiency of WASP, WIP or both did not interfere with selectin-dependent rolling or integrin-dependent adhesion of T cells in vitro. Chemotaxis to stromal cell-derived factor-1a (SDF-1a) in vitro was mildly reduced in T cells from WASP / mice. In contrast, it was significantly impaired in T cells from WIP / mice and severely reduced in T cells from DKO mice. Cellular F-actin increase following SDF-1a stimulation was normal in WASP / and WIP / T cells, but severely reduced in T cells from DKO mice. Actin re-organization and polarization in response to SDF-1a was abnormal in T cells from all knockout mice. Early biochemical events following SDF-1a stimulation that are important for chemotaxis and that included phosphorylation of Lck, cofilin, PAK1 and extracellular regulated kinase (Erk) and GTP loading of Rac-1 were examined in T cells from DKO mice and found to be normal. These results suggest that WASP and WIP are not essential for T lymphocyte rolling and adhesion, but play important and partially redundant roles in T cell chemotaxis in vitro and homing in vivo and function downstream of small GTPases.Oxford University Press2005info:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1093/intimm/dxh310http://uvadoc.uva.es/handle/10324/9838reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidIngléshttps://academic.oup.com/intimm/article/18/2/221/664515info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/3.0/oai:uvadoc.uva.es:10324/98382026-06-13T12:44:47Z
dc.title.none.fl_str_mv WIP and WASP play complementary roles in T cell homing and chemotaxis to SDF-1a
title WIP and WASP play complementary roles in T cell homing and chemotaxis to SDF-1a
spellingShingle WIP and WASP play complementary roles in T cell homing and chemotaxis to SDF-1a
Gallego, María Dolores
Biología celular
title_short WIP and WASP play complementary roles in T cell homing and chemotaxis to SDF-1a
title_full WIP and WASP play complementary roles in T cell homing and chemotaxis to SDF-1a
title_fullStr WIP and WASP play complementary roles in T cell homing and chemotaxis to SDF-1a
title_full_unstemmed WIP and WASP play complementary roles in T cell homing and chemotaxis to SDF-1a
title_sort WIP and WASP play complementary roles in T cell homing and chemotaxis to SDF-1a
dc.creator.none.fl_str_mv Gallego, María Dolores
Fuente García, Miguel Ángel de la
Antón, Inés María
Snapper, Scott B.
Fuhlbrigge, Robert
Geha, Raif S.
author Gallego, María Dolores
author_facet Gallego, María Dolores
Fuente García, Miguel Ángel de la
Antón, Inés María
Snapper, Scott B.
Fuhlbrigge, Robert
Geha, Raif S.
author_role author
author2 Fuente García, Miguel Ángel de la
Antón, Inés María
Snapper, Scott B.
Fuhlbrigge, Robert
Geha, Raif S.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biología celular
topic Biología celular
description Producción Científica
publishDate 2005
dc.date.none.fl_str_mv 2005
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://doi.org/10.1093/intimm/dxh310
http://uvadoc.uva.es/handle/10324/9838
url https://doi.org/10.1093/intimm/dxh310
http://uvadoc.uva.es/handle/10324/9838
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://academic.oup.com/intimm/article/18/2/221/664515
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/3.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/3.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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