Opposite regulation of cannabinoid CB1 and CB2 receptors in the prefrontal cortex of rats treated with cocaine during adolescence

The endocannabinoid system is implicated in the neurobiology of cocaine addiction, although it is not clear how cocaine regulates brain CB1 and CB2 receptors, especially during adolescence, a critical moment for shaping adult response to drug use. This study evaluated CB1 and CB2 protein levels in p...

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Detalles Bibliográficos
Autores: García-Cabrerizo, Rubén, García-Fuster, M Julia
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Conselleria de Salut i Consum del Govern de les Illes Balears
Repositorio:Docusalut
Idioma:inglés
OAI Identifier:oai:docusalut.com:20.500.13003/21625
Acceso en línea:https://hdl.handle.net/20.500.13003/21625
Access Level:acceso abierto
Palabra clave:Male
Age Factors
Cocaine
Prefrontal Cortex
Animals
Hippocampus
Gene Expression Regulation
Rats, Sprague-Dawley
Receptor, Cannabinoid, CB1
Receptor, Cannabinoid, CB2
Regulación de la Expresión Génica
Animales
Receptor Cannabinoide CB1
Receptor Cannabinoide CB2
Ratas Sprague-Dawley
Hipocampo
Factores de Edad
Cocaína
Corteza Prefrontal
Masculino
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network_acronym_str ES
network_name_str España
repository_id_str
spelling Opposite regulation of cannabinoid CB1 and CB2 receptors in the prefrontal cortex of rats treated with cocaine during adolescenceGarcía-Cabrerizo, RubénGarcía-Fuster, M JuliaMaleAge FactorsCocainePrefrontal CortexAnimalsHippocampusGene Expression RegulationRats, Sprague-DawleyReceptor, Cannabinoid, CB1Receptor, Cannabinoid, CB2Regulación de la Expresión GénicaAnimalesReceptor Cannabinoide CB1Receptor Cannabinoide CB2Ratas Sprague-DawleyHipocampoFactores de EdadCocaínaCorteza PrefrontalMasculinoThe endocannabinoid system is implicated in the neurobiology of cocaine addiction, although it is not clear how cocaine regulates brain CB1 and CB2 receptors, especially during adolescence, a critical moment for shaping adult response to drug use. This study evaluated CB1 and CB2 protein levels in prefrontal cortex (PFC) and hippocampus (HC) by western blot analysis with specific and validated antibodies: (1) basally during adolescence (post-natal day PND 40, PND 47, PND 54), (2) by a sensitizing regimen of cocaine (15mg/kg, 7 days, i.p.) during different windows of adolescence vulnerability (PND 33-39, PND 40-46, PND 47-53), and (3) following repeated cocaine administration during adolescence (PND 33-39) in adulthood (PND 64). The results demonstrated a dynamic and opposite basal modulation of CB1 and CB2 receptors in PFC and HC during adolescence. CB1 receptor levels were increased while CB2 receptors were decreased as compared to adulthood with asymptotes values around mid adolescence (PND 47) both in PFC (CB1: +45±22, p<0.05; CB2: -24±6%, p<0.05) and HC (CB1: +53±23, p<0.05; CB2: -20±8%, p<0.05). Interestingly, cocaine only altered CB1 (+55±10%, p<0.05) and CB2 (-25±10%, p<0.05) receptors when administered during early adolescence and only in PFC. However, the changes observed in PFC by repeated cocaine administration in adolescence were transient and did not endure into adulthood. These results identified a period of vulnerability during adolescence at which cocaine dysregulated the content of CB receptors in PFC, suggesting an opposite role for these receptors in the effects mediated by cocaine.Elsevier20162016-02-2620162016-02-26research articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.13003/21625reponame:Docusalutinstname:Conselleria de Salut i Consum del Govern de les Illes BalearsInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docusalut.com:20.500.13003/216252026-06-22T12:44:07Z
dc.title.none.fl_str_mv Opposite regulation of cannabinoid CB1 and CB2 receptors in the prefrontal cortex of rats treated with cocaine during adolescence
title Opposite regulation of cannabinoid CB1 and CB2 receptors in the prefrontal cortex of rats treated with cocaine during adolescence
spellingShingle Opposite regulation of cannabinoid CB1 and CB2 receptors in the prefrontal cortex of rats treated with cocaine during adolescence
García-Cabrerizo, Rubén
Male
Age Factors
Cocaine
Prefrontal Cortex
Animals
Hippocampus
Gene Expression Regulation
Rats, Sprague-Dawley
Receptor, Cannabinoid, CB1
Receptor, Cannabinoid, CB2
Regulación de la Expresión Génica
Animales
Receptor Cannabinoide CB1
Receptor Cannabinoide CB2
Ratas Sprague-Dawley
Hipocampo
Factores de Edad
Cocaína
Corteza Prefrontal
Masculino
title_short Opposite regulation of cannabinoid CB1 and CB2 receptors in the prefrontal cortex of rats treated with cocaine during adolescence
title_full Opposite regulation of cannabinoid CB1 and CB2 receptors in the prefrontal cortex of rats treated with cocaine during adolescence
title_fullStr Opposite regulation of cannabinoid CB1 and CB2 receptors in the prefrontal cortex of rats treated with cocaine during adolescence
title_full_unstemmed Opposite regulation of cannabinoid CB1 and CB2 receptors in the prefrontal cortex of rats treated with cocaine during adolescence
title_sort Opposite regulation of cannabinoid CB1 and CB2 receptors in the prefrontal cortex of rats treated with cocaine during adolescence
dc.creator.none.fl_str_mv García-Cabrerizo, Rubén
García-Fuster, M Julia
author García-Cabrerizo, Rubén
author_facet García-Cabrerizo, Rubén
García-Fuster, M Julia
author_role author
author2 García-Fuster, M Julia
author2_role author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Male
Age Factors
Cocaine
Prefrontal Cortex
Animals
Hippocampus
Gene Expression Regulation
Rats, Sprague-Dawley
Receptor, Cannabinoid, CB1
Receptor, Cannabinoid, CB2
Regulación de la Expresión Génica
Animales
Receptor Cannabinoide CB1
Receptor Cannabinoide CB2
Ratas Sprague-Dawley
Hipocampo
Factores de Edad
Cocaína
Corteza Prefrontal
Masculino
topic Male
Age Factors
Cocaine
Prefrontal Cortex
Animals
Hippocampus
Gene Expression Regulation
Rats, Sprague-Dawley
Receptor, Cannabinoid, CB1
Receptor, Cannabinoid, CB2
Regulación de la Expresión Génica
Animales
Receptor Cannabinoide CB1
Receptor Cannabinoide CB2
Ratas Sprague-Dawley
Hipocampo
Factores de Edad
Cocaína
Corteza Prefrontal
Masculino
description The endocannabinoid system is implicated in the neurobiology of cocaine addiction, although it is not clear how cocaine regulates brain CB1 and CB2 receptors, especially during adolescence, a critical moment for shaping adult response to drug use. This study evaluated CB1 and CB2 protein levels in prefrontal cortex (PFC) and hippocampus (HC) by western blot analysis with specific and validated antibodies: (1) basally during adolescence (post-natal day PND 40, PND 47, PND 54), (2) by a sensitizing regimen of cocaine (15mg/kg, 7 days, i.p.) during different windows of adolescence vulnerability (PND 33-39, PND 40-46, PND 47-53), and (3) following repeated cocaine administration during adolescence (PND 33-39) in adulthood (PND 64). The results demonstrated a dynamic and opposite basal modulation of CB1 and CB2 receptors in PFC and HC during adolescence. CB1 receptor levels were increased while CB2 receptors were decreased as compared to adulthood with asymptotes values around mid adolescence (PND 47) both in PFC (CB1: +45±22, p<0.05; CB2: -24±6%, p<0.05) and HC (CB1: +53±23, p<0.05; CB2: -20±8%, p<0.05). Interestingly, cocaine only altered CB1 (+55±10%, p<0.05) and CB2 (-25±10%, p<0.05) receptors when administered during early adolescence and only in PFC. However, the changes observed in PFC by repeated cocaine administration in adolescence were transient and did not endure into adulthood. These results identified a period of vulnerability during adolescence at which cocaine dysregulated the content of CB receptors in PFC, suggesting an opposite role for these receptors in the effects mediated by cocaine.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-02-26
2016
2016-02-26
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.13003/21625
url https://hdl.handle.net/20.500.13003/21625
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docusalut
instname:Conselleria de Salut i Consum del Govern de les Illes Balears
instname_str Conselleria de Salut i Consum del Govern de les Illes Balears
reponame_str Docusalut
collection Docusalut
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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