Nano-formulation endows quorum quenching enzyme-antibiotic hybrids with improved antibacterial and antibiofilm activities against pseudomonas aeruginosa

The emergence of antibiotic resistant bacteria coupled with the shortage of efficient antibacterials is one of the most serious unresolved problems for modern medicine. In this study, the nano-hybridization of the clinically relevant antibiotic, gentamicin, with the bacterial pro-pathological cell-t...

Descripción completa

Detalles Bibliográficos
Autores: Ivanova, Kristina Dimitrova|||0000-0001-9158-4088, Ivanova, Aleksandra Asenova|||0000-0002-7210-8905, Hoyo, Javier, Pérez Rafael, Silvia|||0000-0001-9658-6090, Tzanov, Tzanko|||0000-0002-8568-1110
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/375468
Acceso en línea:https://hdl.handle.net/2117/375468
https://dx.doi.org/10.3390/ijms23147632
Access Level:acceso abierto
Palabra clave:Drug resistance in microorganisms
Gentamicin
Acylase
Nano-spheres
Quorum sensing inhibition
Antibacterial and antibiofilm activity
Microorganismes -- Resistència als medicaments
Gentamicina
Àrees temàtiques de la UPC::Enginyeria química
id ES_fdf0072a137dfe0a9bb49190763cd1de
oai_identifier_str oai:upcommons.upc.edu:2117/375468
network_acronym_str ES
network_name_str España
repository_id_str
spelling Nano-formulation endows quorum quenching enzyme-antibiotic hybrids with improved antibacterial and antibiofilm activities against pseudomonas aeruginosaIvanova, Kristina Dimitrova|||0000-0001-9158-4088Ivanova, Aleksandra Asenova|||0000-0002-7210-8905Hoyo, JavierPérez Rafael, Silvia|||0000-0001-9658-6090Tzanov, Tzanko|||0000-0002-8568-1110Drug resistance in microorganismsGentamicinGentamicinAcylaseNano-spheresQuorum sensing inhibitionAntibacterial and antibiofilm activityMicroorganismes -- Resistència als medicamentsGentamicinaÀrees temàtiques de la UPC::Enginyeria químicaThe emergence of antibiotic resistant bacteria coupled with the shortage of efficient antibacterials is one of the most serious unresolved problems for modern medicine. In this study, the nano-hybridization of the clinically relevant antibiotic, gentamicin, with the bacterial pro-pathological cell-to-cell communication-quenching enzyme, acylase, is innovatively employed to increase its antimicrobial efficiency against Pseudomonas aeruginosa planktonic cells and biofilms. The sonochemically generated hybrid gentamicin/acylase nano-spheres (GeN_AC NSs) showed a 16-fold improved bactericidal activity when compared with the antibiotic in bulk form, due to the enhanced physical interaction and disruption of the P. aeruginosa cell membrane. The nano-hybrids attenuated 97 ± 1.8% of the quorum sensing-regulated virulence factors’ production and inhibited the bacterium biofilm formation in an eight-fold lower concentration than the stand-alone gentamicin NSs. The P. aeruginosa sensitivity to GeN_AC NSs was also confirmed in a real time assay monitoring the bacterial cells elimination, using a quartz crystal microbalance with dissipation. In protein-enriched conditions mimicking the in vivo application, these hybrid nano-antibacterials maintained their antibacterial and antibiofilm effectiveness at concentrations innocuous to human cells. Therefore, the novel GeN_AC NSs with complementary modes of action show potential for the treatment of P. aeruginosa biofilm infections at a reduced antibiotic dosage.Peer Reviewed20222022-07-1120222022-11-03journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/375468https://dx.doi.org/10.3390/ijms23147632reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3754682026-05-27T15:37:01Z
dc.title.none.fl_str_mv Nano-formulation endows quorum quenching enzyme-antibiotic hybrids with improved antibacterial and antibiofilm activities against pseudomonas aeruginosa
title Nano-formulation endows quorum quenching enzyme-antibiotic hybrids with improved antibacterial and antibiofilm activities against pseudomonas aeruginosa
spellingShingle Nano-formulation endows quorum quenching enzyme-antibiotic hybrids with improved antibacterial and antibiofilm activities against pseudomonas aeruginosa
Ivanova, Kristina Dimitrova|||0000-0001-9158-4088
Drug resistance in microorganisms
Gentamicin
Gentamicin
Acylase
Nano-spheres
Quorum sensing inhibition
Antibacterial and antibiofilm activity
Microorganismes -- Resistència als medicaments
Gentamicina
Àrees temàtiques de la UPC::Enginyeria química
title_short Nano-formulation endows quorum quenching enzyme-antibiotic hybrids with improved antibacterial and antibiofilm activities against pseudomonas aeruginosa
title_full Nano-formulation endows quorum quenching enzyme-antibiotic hybrids with improved antibacterial and antibiofilm activities against pseudomonas aeruginosa
title_fullStr Nano-formulation endows quorum quenching enzyme-antibiotic hybrids with improved antibacterial and antibiofilm activities against pseudomonas aeruginosa
title_full_unstemmed Nano-formulation endows quorum quenching enzyme-antibiotic hybrids with improved antibacterial and antibiofilm activities against pseudomonas aeruginosa
title_sort Nano-formulation endows quorum quenching enzyme-antibiotic hybrids with improved antibacterial and antibiofilm activities against pseudomonas aeruginosa
dc.creator.none.fl_str_mv Ivanova, Kristina Dimitrova|||0000-0001-9158-4088
Ivanova, Aleksandra Asenova|||0000-0002-7210-8905
Hoyo, Javier
Pérez Rafael, Silvia|||0000-0001-9658-6090
Tzanov, Tzanko|||0000-0002-8568-1110
author Ivanova, Kristina Dimitrova|||0000-0001-9158-4088
author_facet Ivanova, Kristina Dimitrova|||0000-0001-9158-4088
Ivanova, Aleksandra Asenova|||0000-0002-7210-8905
Hoyo, Javier
Pérez Rafael, Silvia|||0000-0001-9658-6090
Tzanov, Tzanko|||0000-0002-8568-1110
author_role author
author2 Ivanova, Aleksandra Asenova|||0000-0002-7210-8905
Hoyo, Javier
Pérez Rafael, Silvia|||0000-0001-9658-6090
Tzanov, Tzanko|||0000-0002-8568-1110
author2_role author
author
author
author
dc.subject.none.fl_str_mv Drug resistance in microorganisms
Gentamicin
Gentamicin
Acylase
Nano-spheres
Quorum sensing inhibition
Antibacterial and antibiofilm activity
Microorganismes -- Resistència als medicaments
Gentamicina
Àrees temàtiques de la UPC::Enginyeria química
topic Drug resistance in microorganisms
Gentamicin
Gentamicin
Acylase
Nano-spheres
Quorum sensing inhibition
Antibacterial and antibiofilm activity
Microorganismes -- Resistència als medicaments
Gentamicina
Àrees temàtiques de la UPC::Enginyeria química
description The emergence of antibiotic resistant bacteria coupled with the shortage of efficient antibacterials is one of the most serious unresolved problems for modern medicine. In this study, the nano-hybridization of the clinically relevant antibiotic, gentamicin, with the bacterial pro-pathological cell-to-cell communication-quenching enzyme, acylase, is innovatively employed to increase its antimicrobial efficiency against Pseudomonas aeruginosa planktonic cells and biofilms. The sonochemically generated hybrid gentamicin/acylase nano-spheres (GeN_AC NSs) showed a 16-fold improved bactericidal activity when compared with the antibiotic in bulk form, due to the enhanced physical interaction and disruption of the P. aeruginosa cell membrane. The nano-hybrids attenuated 97 ± 1.8% of the quorum sensing-regulated virulence factors’ production and inhibited the bacterium biofilm formation in an eight-fold lower concentration than the stand-alone gentamicin NSs. The P. aeruginosa sensitivity to GeN_AC NSs was also confirmed in a real time assay monitoring the bacterial cells elimination, using a quartz crystal microbalance with dissipation. In protein-enriched conditions mimicking the in vivo application, these hybrid nano-antibacterials maintained their antibacterial and antibiofilm effectiveness at concentrations innocuous to human cells. Therefore, the novel GeN_AC NSs with complementary modes of action show potential for the treatment of P. aeruginosa biofilm infections at a reduced antibiotic dosage.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-07-11
2022
2022-11-03
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/375468
https://dx.doi.org/10.3390/ijms23147632
url https://hdl.handle.net/2117/375468
https://dx.doi.org/10.3390/ijms23147632
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425638874546177
score 15,301603

Similares en LA Referencia