An immunocytochemical approach to the analysis of the cell division cycle in the rat cerebellar neuroepithelium

Cerebellar neurons are generated from the rhombic lip and the neuroepithelium. In this study, we analyze the histogenesis of the cerebellar neuroepithelium in terms of cellular kinetics. The experimental animals are the offspring of pregnant dams injected with 5-bromo-2ʹ-deoxyuridine (BrdU) on embry...

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Autores: Martí-Clúa, Joaquín|||0000-0002-6774-0092, Rodríguez Vázquez, Lucía|||0000-0003-0151-3142
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:288678
Acceso en línea:https://ddd.uab.cat/record/288678
https://dx.doi.org/urn:doi:10.1080/15384101.2020.1806425
Access Level:acceso abierto
Palabra clave:Prenatal
S-phase
Bromodeoxyuridine
Cell kinetics
Cerebellum
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spelling An immunocytochemical approach to the analysis of the cell division cycle in the rat cerebellar neuroepitheliumMartí-Clúa, Joaquín|||0000-0002-6774-0092Rodríguez Vázquez, Lucía|||0000-0003-0151-3142PrenatalS-phaseBromodeoxyuridineCell kineticsCerebellumCerebellar neurons are generated from the rhombic lip and the neuroepithelium. In this study, we analyze the histogenesis of the cerebellar neuroepithelium in terms of cellular kinetics. The experimental animals are the offspring of pregnant dams injected with 5-bromo-2ʹ-deoxyuridine (BrdU) on embryonic day 13. We infer the fraction of S-phase cells by examining a range of survival times after a single BrdU-exposure and a cumulative BrdU-labeling sequence, which allow for the derivation of cell-cycle parameters and phase durations. The current results indicate that the dose of BrdU employed (35 mg/kg) provides saturation S-phase labeling from at least 1 h after marker delivery. The duration of G2, mitotic phase, and G1 are 1.2, 0.5, and 6.9 h, respectively. The duration for the S-phase, growth fraction, and the whole cycle are obtained on the basis of two proliferative models, steady-state and exponential growth. Both models provided similar results. In conclusion, our results indicate that the steady-state and the cumulative S-phase labeling paradigms can be adopted to analyze cell cycle parameters in the cerebellar neuroepithelium. Current results can help in understanding the regulatory mechanisms of cerebellar histogenesis and the cell biological mechanisms of the proliferative cycle of the neuroepithelium. 22020-01-0120202020-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/288678https://dx.doi.org/urn:doi:10.1080/15384101.2020.1806425reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.https://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2886782026-06-06T12:50:31Z
dc.title.none.fl_str_mv An immunocytochemical approach to the analysis of the cell division cycle in the rat cerebellar neuroepithelium
title An immunocytochemical approach to the analysis of the cell division cycle in the rat cerebellar neuroepithelium
spellingShingle An immunocytochemical approach to the analysis of the cell division cycle in the rat cerebellar neuroepithelium
Martí-Clúa, Joaquín|||0000-0002-6774-0092
Prenatal
S-phase
Bromodeoxyuridine
Cell kinetics
Cerebellum
title_short An immunocytochemical approach to the analysis of the cell division cycle in the rat cerebellar neuroepithelium
title_full An immunocytochemical approach to the analysis of the cell division cycle in the rat cerebellar neuroepithelium
title_fullStr An immunocytochemical approach to the analysis of the cell division cycle in the rat cerebellar neuroepithelium
title_full_unstemmed An immunocytochemical approach to the analysis of the cell division cycle in the rat cerebellar neuroepithelium
title_sort An immunocytochemical approach to the analysis of the cell division cycle in the rat cerebellar neuroepithelium
dc.creator.none.fl_str_mv Martí-Clúa, Joaquín|||0000-0002-6774-0092
Rodríguez Vázquez, Lucía|||0000-0003-0151-3142
author Martí-Clúa, Joaquín|||0000-0002-6774-0092
author_facet Martí-Clúa, Joaquín|||0000-0002-6774-0092
Rodríguez Vázquez, Lucía|||0000-0003-0151-3142
author_role author
author2 Rodríguez Vázquez, Lucía|||0000-0003-0151-3142
author2_role author
dc.subject.none.fl_str_mv Prenatal
S-phase
Bromodeoxyuridine
Cell kinetics
Cerebellum
topic Prenatal
S-phase
Bromodeoxyuridine
Cell kinetics
Cerebellum
description Cerebellar neurons are generated from the rhombic lip and the neuroepithelium. In this study, we analyze the histogenesis of the cerebellar neuroepithelium in terms of cellular kinetics. The experimental animals are the offspring of pregnant dams injected with 5-bromo-2ʹ-deoxyuridine (BrdU) on embryonic day 13. We infer the fraction of S-phase cells by examining a range of survival times after a single BrdU-exposure and a cumulative BrdU-labeling sequence, which allow for the derivation of cell-cycle parameters and phase durations. The current results indicate that the dose of BrdU employed (35 mg/kg) provides saturation S-phase labeling from at least 1 h after marker delivery. The duration of G2, mitotic phase, and G1 are 1.2, 0.5, and 6.9 h, respectively. The duration for the S-phase, growth fraction, and the whole cycle are obtained on the basis of two proliferative models, steady-state and exponential growth. Both models provided similar results. In conclusion, our results indicate that the steady-state and the cumulative S-phase labeling paradigms can be adopted to analyze cell cycle parameters in the cerebellar neuroepithelium. Current results can help in understanding the regulatory mechanisms of cerebellar histogenesis and the cell biological mechanisms of the proliferative cycle of the neuroepithelium.
publishDate 2020
dc.date.none.fl_str_mv 2
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/288678
https://dx.doi.org/urn:doi:10.1080/15384101.2020.1806425
url https://ddd.uab.cat/record/288678
https://dx.doi.org/urn:doi:10.1080/15384101.2020.1806425
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
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https://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
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eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
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