Identification and Assessment of the Potential Allergenicity of 7S Vicilins in Olive (Olea europaea L.) Seeds

Olive seeds, which are a raw material of interest, have been reported to contain 11S seed storage proteins (SSPs). However, the presence of SSPs such as 7S vicilins has not been studied. In this study, following a search in the olive seed transcriptome, 58 sequences corresponding to 7S vicilins were...

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Autores: Jiménez-López, José Carlos, Zafra, Adoración, Palanco, Lucía, Florido, José Fernando, Alché Ramírez, Juan de Dios
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/132395
Acceso en línea:http://hdl.handle.net/10261/132395
Access Level:acceso abierto
Palabra clave:Olivos
Plantas de pólenes alergénicos
Proteínas vegetales
Vicilina
Fiebre del heno
Alérgenos
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spelling Identification and Assessment of the Potential Allergenicity of 7S Vicilins in Olive (Olea europaea L.) SeedsJiménez-López, José CarlosZafra, AdoraciónPalanco, LucíaFlorido, José FernandoAlché Ramírez, Juan de DiosOlivosPlantas de pólenes alergénicosProteínas vegetalesVicilinaFiebre del henoAlérgenosOlive seeds, which are a raw material of interest, have been reported to contain 11S seed storage proteins (SSPs). However, the presence of SSPs such as 7S vicilins has not been studied. In this study, following a search in the olive seed transcriptome, 58 sequences corresponding to 7S vicilins were retrieved. A partial sequence was amplified by PCR from olive seed cDNA and subjected to phylogenetic analysis with other sequences. Structural analysis showed that olive 7S vicilin contains 9 -helixes and 22 -sheets. Additionally, 3D structural analysis displayed good superimposition with vicilin models generated from Pistacia and Sesamum. In order to assess potential allergenicity, T and B epitopes present in these proteins were identified by bioinformatic approaches. Different motifs were observed among the species, as well as some species-specific motifs. Finally, expression analysis of vicilins was carried out in protein extracts obtained from seeds of different species, including the olive. Noticeable bands were observed for all species in the 15–75 kDa MW interval, which were compatible with vicilins. The reactivity of the extracts to sera from patients allergic to nuts was also analysed. The findings with regard to the potential use of olive seed as food are discussed. [EN]This study has been supported by the following grants, partially cofunded by ERDF: P2010-AGR-6274 and P2011-CVI-7487 from the Junta de Andalucía; the 201540E065 Intramural-CSIC and MINECO RETOS-COLABORACIÓN RTC2015-4181-2; and the EU Marie Curie (FP7-PEOPLE-2011-IOF) ref. PIOF-GA-2011-311550.Peer reviewedHindawi Publishing CorporationJunta de AndalucíaMinisterio de Economía, Fomento y Turismo (Chile)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201620162016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/132395reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1155/2016/4946872Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1323952026-05-22T06:33:51Z
dc.title.none.fl_str_mv Identification and Assessment of the Potential Allergenicity of 7S Vicilins in Olive (Olea europaea L.) Seeds
title Identification and Assessment of the Potential Allergenicity of 7S Vicilins in Olive (Olea europaea L.) Seeds
spellingShingle Identification and Assessment of the Potential Allergenicity of 7S Vicilins in Olive (Olea europaea L.) Seeds
Jiménez-López, José Carlos
Olivos
Plantas de pólenes alergénicos
Proteínas vegetales
Vicilina
Fiebre del heno
Alérgenos
title_short Identification and Assessment of the Potential Allergenicity of 7S Vicilins in Olive (Olea europaea L.) Seeds
title_full Identification and Assessment of the Potential Allergenicity of 7S Vicilins in Olive (Olea europaea L.) Seeds
title_fullStr Identification and Assessment of the Potential Allergenicity of 7S Vicilins in Olive (Olea europaea L.) Seeds
title_full_unstemmed Identification and Assessment of the Potential Allergenicity of 7S Vicilins in Olive (Olea europaea L.) Seeds
title_sort Identification and Assessment of the Potential Allergenicity of 7S Vicilins in Olive (Olea europaea L.) Seeds
dc.creator.none.fl_str_mv Jiménez-López, José Carlos
Zafra, Adoración
Palanco, Lucía
Florido, José Fernando
Alché Ramírez, Juan de Dios
author Jiménez-López, José Carlos
author_facet Jiménez-López, José Carlos
Zafra, Adoración
Palanco, Lucía
Florido, José Fernando
Alché Ramírez, Juan de Dios
author_role author
author2 Zafra, Adoración
Palanco, Lucía
Florido, José Fernando
Alché Ramírez, Juan de Dios
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Junta de Andalucía
Ministerio de Economía, Fomento y Turismo (Chile)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Olivos
Plantas de pólenes alergénicos
Proteínas vegetales
Vicilina
Fiebre del heno
Alérgenos
topic Olivos
Plantas de pólenes alergénicos
Proteínas vegetales
Vicilina
Fiebre del heno
Alérgenos
description Olive seeds, which are a raw material of interest, have been reported to contain 11S seed storage proteins (SSPs). However, the presence of SSPs such as 7S vicilins has not been studied. In this study, following a search in the olive seed transcriptome, 58 sequences corresponding to 7S vicilins were retrieved. A partial sequence was amplified by PCR from olive seed cDNA and subjected to phylogenetic analysis with other sequences. Structural analysis showed that olive 7S vicilin contains 9 -helixes and 22 -sheets. Additionally, 3D structural analysis displayed good superimposition with vicilin models generated from Pistacia and Sesamum. In order to assess potential allergenicity, T and B epitopes present in these proteins were identified by bioinformatic approaches. Different motifs were observed among the species, as well as some species-specific motifs. Finally, expression analysis of vicilins was carried out in protein extracts obtained from seeds of different species, including the olive. Noticeable bands were observed for all species in the 15–75 kDa MW interval, which were compatible with vicilins. The reactivity of the extracts to sera from patients allergic to nuts was also analysed. The findings with regard to the potential use of olive seed as food are discussed. [EN]
publishDate 2016
dc.date.none.fl_str_mv 2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/132395
url http://hdl.handle.net/10261/132395
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1155/2016/4946872
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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