Human cerebral organoids: cellular composition and subcellular morphological features

Introduction: Human cerebral organoids (hCOs) derived from pluripotent stem cells are very promising for the study of neurodevelopment and the investigation of the healthy or diseased brain. To help establish hCOs as a powerful research model, it is essential to perform the morphological characteriz...

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Autores: Mateos-Martínez, Patricia, Coronel Lopez, Raquel, Sachse, Martin, González-Sastre, Rosa, Maeso Cuesta, Laura, Rodríguez, María Josefa, Terrón-Orellana, Maria Carmen, López-Alonso, Victoria, Liste-Noya, Isabel
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/25371
Acceso en línea:https://hdl.handle.net/20.500.12105/25371
Access Level:acceso abierto
Palabra clave:Glial cells
Human brain organoids
Human pluripotent stem cells
Mini-brains
Neural stem cells
Neurodevelopment
Transmission electron microscopy
Ultrastructural characterization
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spelling Human cerebral organoids: cellular composition and subcellular morphological featuresMateos-Martínez, PatriciaCoronel Lopez, RaquelSachse, MartinGonzález-Sastre, RosaMaeso Cuesta, LauraRodríguez, María JosefaTerrón-Orellana, Maria CarmenLópez-Alonso, VictoriaListe-Noya, IsabelGlial cellsHuman brain organoidsHuman pluripotent stem cellsMini-brainsNeural stem cellsNeurodevelopmentTransmission electron microscopyUltrastructural characterizationIntroduction: Human cerebral organoids (hCOs) derived from pluripotent stem cells are very promising for the study of neurodevelopment and the investigation of the healthy or diseased brain. To help establish hCOs as a powerful research model, it is essential to perform the morphological characterization of their cellular components in depth. Methods: In this study, we analyzed the cell types consisting of hCOs after culturing for 45 days using immunofluorescence and reverse transcriptase qualitative polymerase chain reaction (RT-qPCR) assays. We also analyzed their subcellular morphological characteristics by transmission electron microscopy (TEM). Results: Our results show the development of proliferative zones to be remarkably similar to those found in human brain development with cells having a polarized structure surrounding a central cavity with tight junctions and cilia. In addition, we describe the presence of immature and mature migrating neurons, astrocytes, oligodendrocyte precursor cells, and microglia-like cells. Discussion: The ultrastructural characterization presented in this study provides valuable information on the structural development and morphology of the hCO, and this information is of general interest for future research on the mechanisms that alter the cell structure or function of hCOs.Frontiers MediaMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)Instituto de Salud Carlos IIIUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)20242024-10-2920242024-01-0120242024-01-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12105/25371reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)InglésengES PID2021-126715OB-I00 Not availableES PI22CIII 00055ES RTI2018-101663-B-100 Not availableES UFIECPY328 22ES PEJ-2021-TL BMD-21001ES UFIECPY-396 19ES PEJ2018-004961 Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/253712026-06-12T12:43:37Z
dc.title.none.fl_str_mv Human cerebral organoids: cellular composition and subcellular morphological features
title Human cerebral organoids: cellular composition and subcellular morphological features
spellingShingle Human cerebral organoids: cellular composition and subcellular morphological features
Mateos-Martínez, Patricia
Glial cells
Human brain organoids
Human pluripotent stem cells
Mini-brains
Neural stem cells
Neurodevelopment
Transmission electron microscopy
Ultrastructural characterization
title_short Human cerebral organoids: cellular composition and subcellular morphological features
title_full Human cerebral organoids: cellular composition and subcellular morphological features
title_fullStr Human cerebral organoids: cellular composition and subcellular morphological features
title_full_unstemmed Human cerebral organoids: cellular composition and subcellular morphological features
title_sort Human cerebral organoids: cellular composition and subcellular morphological features
dc.creator.none.fl_str_mv Mateos-Martínez, Patricia
Coronel Lopez, Raquel
Sachse, Martin
González-Sastre, Rosa
Maeso Cuesta, Laura
Rodríguez, María Josefa
Terrón-Orellana, Maria Carmen
López-Alonso, Victoria
Liste-Noya, Isabel
author Mateos-Martínez, Patricia
author_facet Mateos-Martínez, Patricia
Coronel Lopez, Raquel
Sachse, Martin
González-Sastre, Rosa
Maeso Cuesta, Laura
Rodríguez, María Josefa
Terrón-Orellana, Maria Carmen
López-Alonso, Victoria
Liste-Noya, Isabel
author_role author
author2 Coronel Lopez, Raquel
Sachse, Martin
González-Sastre, Rosa
Maeso Cuesta, Laura
Rodríguez, María Josefa
Terrón-Orellana, Maria Carmen
López-Alonso, Victoria
Liste-Noya, Isabel
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
Instituto de Salud Carlos III
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
dc.subject.none.fl_str_mv Glial cells
Human brain organoids
Human pluripotent stem cells
Mini-brains
Neural stem cells
Neurodevelopment
Transmission electron microscopy
Ultrastructural characterization
topic Glial cells
Human brain organoids
Human pluripotent stem cells
Mini-brains
Neural stem cells
Neurodevelopment
Transmission electron microscopy
Ultrastructural characterization
description Introduction: Human cerebral organoids (hCOs) derived from pluripotent stem cells are very promising for the study of neurodevelopment and the investigation of the healthy or diseased brain. To help establish hCOs as a powerful research model, it is essential to perform the morphological characterization of their cellular components in depth. Methods: In this study, we analyzed the cell types consisting of hCOs after culturing for 45 days using immunofluorescence and reverse transcriptase qualitative polymerase chain reaction (RT-qPCR) assays. We also analyzed their subcellular morphological characteristics by transmission electron microscopy (TEM). Results: Our results show the development of proliferative zones to be remarkably similar to those found in human brain development with cells having a polarized structure surrounding a central cavity with tight junctions and cilia. In addition, we describe the presence of immature and mature migrating neurons, astrocytes, oligodendrocyte precursor cells, and microglia-like cells. Discussion: The ultrastructural characterization presented in this study provides valuable information on the structural development and morphology of the hCO, and this information is of general interest for future research on the mechanisms that alter the cell structure or function of hCOs.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-10-29
2024
2024-01-01
2024
2024-01-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12105/25371
url https://hdl.handle.net/20.500.12105/25371
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv ES PID2021-126715OB-I00 Not available
ES PI22CIII 00055
ES RTI2018-101663-B-100 Not available
ES UFIECPY328 22
ES PEJ-2021-TL BMD-21001
ES UFIECPY-396 19
ES PEJ2018-004961 Not available
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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