Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials

The importance of atherosclerosis is driving research to create improved diagnostic tools based on molecular imaging. Pretargeted imaging is the use of bioorthogonal probes that selectively accumulate upon reaction with a pre-modified biomolecule in vivo. To date, this very promising approach has no...

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Autores: Pellico, J., Fernández-Barahona, I., Benito, M., Gaitán-Simón, Á., Gutiérrez, L., Ruiz-Cabello, J., Herranz, F.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Universidad de Zaragoza
Repositorio:Zaguán. Repositorio Digital de la Universidad de Zaragoza
OAI Identifier:oai:zaguan.unizar.es:87537
Acceso en línea:http://zaguan.unizar.es/record/87537
Access Level:acceso abierto
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spelling Unambiguous detection of atherosclerosis using bioorthogonal nanomaterialsPellico, J.Fernández-Barahona, I.Benito, M.Gaitán-Simón, Á.Gutiérrez, L.Ruiz-Cabello, J.Herranz, F.The importance of atherosclerosis is driving research to create improved diagnostic tools based on molecular imaging. Pretargeted imaging is the use of bioorthogonal probes that selectively accumulate upon reaction with a pre-modified biomolecule in vivo. To date, this very promising approach has not been applied to atherosclerosis. Neither has been the use of a single nano-radiomaterial for PET / T1-MR imaging of atherosclerosis. Here, we synthesized bioorthogonal nano-radiomaterials for in vivo pretargeted molecular imaging in a mouse model of atherosclerosis. Based on tetrazine-ligation, these functionalized 68Ga iron oxide nano-radiomaterials provide simultaneous PET and T1-MRI signals and selectively accumulate in atherosclerotic plaques in mice sequentially injected with trans-cyclooctene-modified antibodies against oxidized LDL followed by the hybrid nano-radiomaterial. Our results demonstrate the ability of this approach to unambiguously detect atherosclerosis. Furthermore, we show the first example of how hybrid imaging can be used for pretargeted bioorthogonal molecular imaging with nanomaterials.2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://zaguan.unizar.es/record/87537reponame:Zaguán. Repositorio Digital de la Universidad de Zaragozainstname:Universidad de ZaragozaInglésinfo:eu-repo/grantAgreement/ES/ISCIII/DTS16-00059info:eu-repo/grantAgreement/ES/MEIC/SAF2016-79593-Pinfo:eu-repo/grantAgreement/ES/MINECO/RYC-2014-15512info:eu-repo/semantics/openAccessoai:zaguan.unizar.es:875372026-05-29T13:59:51Z
dc.title.none.fl_str_mv Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials
title Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials
spellingShingle Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials
Pellico, J.
title_short Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials
title_full Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials
title_fullStr Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials
title_full_unstemmed Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials
title_sort Unambiguous detection of atherosclerosis using bioorthogonal nanomaterials
dc.creator.none.fl_str_mv Pellico, J.
Fernández-Barahona, I.
Benito, M.
Gaitán-Simón, Á.
Gutiérrez, L.
Ruiz-Cabello, J.
Herranz, F.
author Pellico, J.
author_facet Pellico, J.
Fernández-Barahona, I.
Benito, M.
Gaitán-Simón, Á.
Gutiérrez, L.
Ruiz-Cabello, J.
Herranz, F.
author_role author
author2 Fernández-Barahona, I.
Benito, M.
Gaitán-Simón, Á.
Gutiérrez, L.
Ruiz-Cabello, J.
Herranz, F.
author2_role author
author
author
author
author
author
description The importance of atherosclerosis is driving research to create improved diagnostic tools based on molecular imaging. Pretargeted imaging is the use of bioorthogonal probes that selectively accumulate upon reaction with a pre-modified biomolecule in vivo. To date, this very promising approach has not been applied to atherosclerosis. Neither has been the use of a single nano-radiomaterial for PET / T1-MR imaging of atherosclerosis. Here, we synthesized bioorthogonal nano-radiomaterials for in vivo pretargeted molecular imaging in a mouse model of atherosclerosis. Based on tetrazine-ligation, these functionalized 68Ga iron oxide nano-radiomaterials provide simultaneous PET and T1-MRI signals and selectively accumulate in atherosclerotic plaques in mice sequentially injected with trans-cyclooctene-modified antibodies against oxidized LDL followed by the hybrid nano-radiomaterial. Our results demonstrate the ability of this approach to unambiguously detect atherosclerosis. Furthermore, we show the first example of how hybrid imaging can be used for pretargeted bioorthogonal molecular imaging with nanomaterials.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://zaguan.unizar.es/record/87537
url http://zaguan.unizar.es/record/87537
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/ES/ISCIII/DTS16-00059
info:eu-repo/grantAgreement/ES/MEIC/SAF2016-79593-P
info:eu-repo/grantAgreement/ES/MINECO/RYC-2014-15512
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv
publisher.none.fl_str_mv
dc.source.none.fl_str_mv reponame:Zaguán. Repositorio Digital de la Universidad de Zaragoza
instname:Universidad de Zaragoza
instname_str Universidad de Zaragoza
reponame_str Zaguán. Repositorio Digital de la Universidad de Zaragoza
collection Zaguán. Repositorio Digital de la Universidad de Zaragoza
repository.name.fl_str_mv
repository.mail.fl_str_mv
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