Exploring the Interplay Between TCR Signaling and its Regulation by Complement in αβT Cells

The role of the complement system during the innate immune response is well known. Nonetheless, complement acts also indirectly at the interface between innate and adaptive immunity. In addition, a solid body of evidence supports the relevance of its direct involvement in adaptive immune responses....

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Detalles Bibliográficos
Autor: Chacón Arguedas, Carlos Daniel
Tipo de recurso: tesis doctoral
Fecha de publicación:2025
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/129422
Acceso en línea:https://hdl.handle.net/20.500.14352/129422
Access Level:acceso abierto
Palabra clave:577.2(043.2)
Biología molecular
Molecular biology
Biología molecular (Biología)
2302.21 Biología Molecular
Descripción
Sumario:The role of the complement system during the innate immune response is well known. Nonetheless, complement acts also indirectly at the interface between innate and adaptive immunity. In addition, a solid body of evidence supports the relevance of its direct involvement in adaptive immune responses. For instance, the B cell antigen receptor (BCR) and surface complement receptors (CR) such as CR2 (CD21) crosstalk when engaged by C3b-opsonized antigens, leading in humans to an inhibitory effect on BCR activation and thus weaker antibody responses. Indeed, C3 deficiency impairs B cell differentiation to memory cells.T cells also integrate signals from various sources to regulate immune responses, including for instance those received through the TCR and CD28. Giving the relevant role of CR in B cells, many reports support that membrane CR and complement regulators (Creg) are important rheostats of T cell activation. However, the interaction between T cell and complement in humans is just beginning to be explored. The aim of this work was to study the expression and signaling of CR and Creg in primary αβ T lymphocytes and T cell lines before and after stimulation via TCR, as well as the effect of C3 on TCR-mediated activation and differentiation...