Lysozyme–aunps interactions: Determination of binding free energy

Investigation and optimization of lysozyme (Lys) adsorption onto gold nanoparticles, AuNPs, were carried out. The purpose of this study is to determine the magnitude of the AuNPs– lysozyme interaction in aqueous media by simple spectrophotometric means, and to obtain the free energy of binding of th...

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Detalhes bibliográficos
Autores: Gomes, Axel, Carnerero Panduro, José María, Jiménez Ruiz, Aila, Grueso Molina, Elia María, Giráldez Pérez, Rosa María, Prado Gotor, Rafael
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Recursos:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/128803
Acesso em linha:https://hdl.handle.net/11441/128803
https://doi.org/10.3390/nano11082139
Access Level:acceso abierto
Palavra-chave:Binding free energy
Gold nanoparticles
Lysozyme
Nanoparticle size
Surface plasmon resonance
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spelling Lysozyme–aunps interactions: Determination of binding free energyGomes, AxelCarnerero Panduro, José MaríaJiménez Ruiz, AilaGrueso Molina, Elia MaríaGiráldez Pérez, Rosa MaríaPrado Gotor, RafaelBinding free energyGold nanoparticlesLysozymeNanoparticle sizeSurface plasmon resonanceInvestigation and optimization of lysozyme (Lys) adsorption onto gold nanoparticles, AuNPs, were carried out. The purpose of this study is to determine the magnitude of the AuNPs– lysozyme interaction in aqueous media by simple spectrophotometric means, and to obtain the free energy of binding of the system for the first time. In order to explore the possibilities of gold nanoparticles for sensing lysozyme in aqueous media, the stability of the samples and the influence of the gold and nanoparticle concentrations in the detection limit were studied. ζ potential measurements and the shift of the surface plasmon band showed a state of saturation with an average number of 55 Lys per gold nanoparticle. Lysozyme–AuNPs interactions induce aggregation of citrate-stabilized AuNPs at low concentrations by neutering the negative charges of citrate anions; from those aggregation data, the magnitude of the interactions has been measured by using Benesi–Hildebrand plots. However, at higher protein concentrations aggregation has been found to decrease. Although the nanocluster morphology remains unchanged in the presence of Lys, slight conformational changes of the protein occur. The influence of the size of the nanoclusters was also investigated for 5, 10, and 20 nm AuNPs, and 10 nm AuNPs was found the most appropriate.Junta de Andalucía P08-FQM-03623Multidisciplinary Digital Publishing Institute (MDPI)Química Física2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/128803https://doi.org/10.3390/nano11082139reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésNanomaterials, 11 (8), 2139.P08-FQM-03623https://doi.org/10.3390/nano11082139info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1288032026-06-17T12:51:07Z
dc.title.none.fl_str_mv Lysozyme–aunps interactions: Determination of binding free energy
title Lysozyme–aunps interactions: Determination of binding free energy
spellingShingle Lysozyme–aunps interactions: Determination of binding free energy
Gomes, Axel
Binding free energy
Gold nanoparticles
Lysozyme
Nanoparticle size
Surface plasmon resonance
title_short Lysozyme–aunps interactions: Determination of binding free energy
title_full Lysozyme–aunps interactions: Determination of binding free energy
title_fullStr Lysozyme–aunps interactions: Determination of binding free energy
title_full_unstemmed Lysozyme–aunps interactions: Determination of binding free energy
title_sort Lysozyme–aunps interactions: Determination of binding free energy
dc.creator.none.fl_str_mv Gomes, Axel
Carnerero Panduro, José María
Jiménez Ruiz, Aila
Grueso Molina, Elia María
Giráldez Pérez, Rosa María
Prado Gotor, Rafael
author Gomes, Axel
author_facet Gomes, Axel
Carnerero Panduro, José María
Jiménez Ruiz, Aila
Grueso Molina, Elia María
Giráldez Pérez, Rosa María
Prado Gotor, Rafael
author_role author
author2 Carnerero Panduro, José María
Jiménez Ruiz, Aila
Grueso Molina, Elia María
Giráldez Pérez, Rosa María
Prado Gotor, Rafael
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Química Física
dc.subject.none.fl_str_mv Binding free energy
Gold nanoparticles
Lysozyme
Nanoparticle size
Surface plasmon resonance
topic Binding free energy
Gold nanoparticles
Lysozyme
Nanoparticle size
Surface plasmon resonance
description Investigation and optimization of lysozyme (Lys) adsorption onto gold nanoparticles, AuNPs, were carried out. The purpose of this study is to determine the magnitude of the AuNPs– lysozyme interaction in aqueous media by simple spectrophotometric means, and to obtain the free energy of binding of the system for the first time. In order to explore the possibilities of gold nanoparticles for sensing lysozyme in aqueous media, the stability of the samples and the influence of the gold and nanoparticle concentrations in the detection limit were studied. ζ potential measurements and the shift of the surface plasmon band showed a state of saturation with an average number of 55 Lys per gold nanoparticle. Lysozyme–AuNPs interactions induce aggregation of citrate-stabilized AuNPs at low concentrations by neutering the negative charges of citrate anions; from those aggregation data, the magnitude of the interactions has been measured by using Benesi–Hildebrand plots. However, at higher protein concentrations aggregation has been found to decrease. Although the nanocluster morphology remains unchanged in the presence of Lys, slight conformational changes of the protein occur. The influence of the size of the nanoclusters was also investigated for 5, 10, and 20 nm AuNPs, and 10 nm AuNPs was found the most appropriate.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/128803
https://doi.org/10.3390/nano11082139
url https://hdl.handle.net/11441/128803
https://doi.org/10.3390/nano11082139
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Nanomaterials, 11 (8), 2139.
P08-FQM-03623
https://doi.org/10.3390/nano11082139
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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