[Raw Data] Lipidomic effects of bisphenol A in zebrafish eleutheroembryos during the yolk sac absorption stage

Previous studies on the effects of bisphenol A (BPA; 4,4'-(propane-2,2-diyl)diphenol) in zebrafish identified lipid metabolism as particularly affected by BPA exposure. We perform here a lipidomic analysis in zebrafish eleutheroembryos exposed up to 8 mg/L of BPA from day 2 to day 6 post fertil...

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Detalles Bibliográficos
Autores: Martínez, Rubén, Navarro-Martín, Laia, Van Antro, Morgane, Fuertes, Inmaculada, Casado, Marta, Barata Martí, Carlos, Piña, Benjamín
Tipo de recurso: conjunto de datos
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/192701
Acceso en línea:http://hdl.handle.net/10261/192701
Access Level:acceso abierto
Palabra clave:Mass spectrometry
Zebrafish
Thin layer chromatography
Yolk sac malabsorption
Adverse outcome pathway
Mode of action
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spelling [Raw Data] Lipidomic effects of bisphenol A in zebrafish eleutheroembryos during the yolk sac absorption stageMartínez, RubénNavarro-Martín, LaiaVan Antro, MorganeFuertes, InmaculadaCasado, MartaBarata Martí, CarlosPiña, BenjamínMass spectrometryZebrafishThin layer chromatographyYolk sac malabsorptionAdverse outcome pathwayMode of actionPrevious studies on the effects of bisphenol A (BPA; 4,4'-(propane-2,2-diyl)diphenol) in zebrafish identified lipid metabolism as particularly affected by BPA exposure. We perform here a lipidomic analysis in zebrafish eleutheroembryos exposed up to 8 mg/L of BPA from day 2 to day 6 post fertilization. Total lipids were analyzed by high-performance thin layer chromatography as wide-range preliminary screening. Selected conditions were used to obtain a high-quality lipid profile using ultra high-performance liquid chromatography/time-of-flight mass spectrometry. BPA specifically impaired the physiological consumption of triglycerides (TG), diglycerides (DG), phosphatidylcholines (PC) and phosphatidylinositols (PI), most of them yolk sac-related lipids. These effects seemed more related to a decrease in energy consumption in BPA exposed individuals than to an impairment of lipid transport from the yolk sac. Integration of these results with previous morphological, biochemical, transcriptomic, metabolomic and behavioral data suggests that changes on lipid metabolism first appeared at very low BPA concentrations, and that they propagated across different organization levels. The analysis also suggests that the disruption of different signalling pathways by BPA results in a combination of adverse outcomes (estrogenic and lipid disruption, eye development), making it advisable a tighter limitation on BPA exposure limits for both wildlife and human populations.This work was supported by the European Research Council under the European Union's Seventh Framework Programme (FP/ 2007-2013)/ERC Grant Agreement n. 320737. Some part of this study was also supported by grants from the Spanish Ministry of Economy and Competitiveness (CTQ2014-56777-R) and the Spanish Ministry of Science, Innovation and University (RTI2018-096175-B-I00). RM was supported by a FPU predoctoral fellow from the Spanish Ministry of Education and Science (ref. FPU15/03332). IF was supported by a FPI predoctoral fellow from the Spanish Ministry of Economy and Competitiveness (FPI-MICINN BES-2015-075023). We thank Juan Carlos Navarro and Josefina Casas for his valuable advice and expertise in the optimization of the thin-layer chromatography technique and liquid chromatography/ mass spectrometry, respectively.Peer reviewedEuropean Research CouncilMinisterio de Economía y Competitividad (España)Navarro-Martín, Laia [0000-0001-6554-8833]Barata Martí, Carlos [0000-0002-3360-0729]PIÑA CAPO, BENJAMIN CAYETANO [0000-0001-9216-2768]Casado, Marta [0000-0002-7349-107X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192019info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/192701reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/FP7/320737info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2014-56777-RSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1927012026-05-22T06:33:51Z
dc.title.none.fl_str_mv [Raw Data] Lipidomic effects of bisphenol A in zebrafish eleutheroembryos during the yolk sac absorption stage
title [Raw Data] Lipidomic effects of bisphenol A in zebrafish eleutheroembryos during the yolk sac absorption stage
spellingShingle [Raw Data] Lipidomic effects of bisphenol A in zebrafish eleutheroembryos during the yolk sac absorption stage
Martínez, Rubén
Mass spectrometry
Zebrafish
Thin layer chromatography
Yolk sac malabsorption
Adverse outcome pathway
Mode of action
title_short [Raw Data] Lipidomic effects of bisphenol A in zebrafish eleutheroembryos during the yolk sac absorption stage
title_full [Raw Data] Lipidomic effects of bisphenol A in zebrafish eleutheroembryos during the yolk sac absorption stage
title_fullStr [Raw Data] Lipidomic effects of bisphenol A in zebrafish eleutheroembryos during the yolk sac absorption stage
title_full_unstemmed [Raw Data] Lipidomic effects of bisphenol A in zebrafish eleutheroembryos during the yolk sac absorption stage
title_sort [Raw Data] Lipidomic effects of bisphenol A in zebrafish eleutheroembryos during the yolk sac absorption stage
dc.creator.none.fl_str_mv Martínez, Rubén
Navarro-Martín, Laia
Van Antro, Morgane
Fuertes, Inmaculada
Casado, Marta
Barata Martí, Carlos
Piña, Benjamín
author Martínez, Rubén
author_facet Martínez, Rubén
Navarro-Martín, Laia
Van Antro, Morgane
Fuertes, Inmaculada
Casado, Marta
Barata Martí, Carlos
Piña, Benjamín
author_role author
author2 Navarro-Martín, Laia
Van Antro, Morgane
Fuertes, Inmaculada
Casado, Marta
Barata Martí, Carlos
Piña, Benjamín
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Research Council
Ministerio de Economía y Competitividad (España)
Navarro-Martín, Laia [0000-0001-6554-8833]
Barata Martí, Carlos [0000-0002-3360-0729]
PIÑA CAPO, BENJAMIN CAYETANO [0000-0001-9216-2768]
Casado, Marta [0000-0002-7349-107X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Mass spectrometry
Zebrafish
Thin layer chromatography
Yolk sac malabsorption
Adverse outcome pathway
Mode of action
topic Mass spectrometry
Zebrafish
Thin layer chromatography
Yolk sac malabsorption
Adverse outcome pathway
Mode of action
description Previous studies on the effects of bisphenol A (BPA; 4,4'-(propane-2,2-diyl)diphenol) in zebrafish identified lipid metabolism as particularly affected by BPA exposure. We perform here a lipidomic analysis in zebrafish eleutheroembryos exposed up to 8 mg/L of BPA from day 2 to day 6 post fertilization. Total lipids were analyzed by high-performance thin layer chromatography as wide-range preliminary screening. Selected conditions were used to obtain a high-quality lipid profile using ultra high-performance liquid chromatography/time-of-flight mass spectrometry. BPA specifically impaired the physiological consumption of triglycerides (TG), diglycerides (DG), phosphatidylcholines (PC) and phosphatidylinositols (PI), most of them yolk sac-related lipids. These effects seemed more related to a decrease in energy consumption in BPA exposed individuals than to an impairment of lipid transport from the yolk sac. Integration of these results with previous morphological, biochemical, transcriptomic, metabolomic and behavioral data suggests that changes on lipid metabolism first appeared at very low BPA concentrations, and that they propagated across different organization levels. The analysis also suggests that the disruption of different signalling pathways by BPA results in a combination of adverse outcomes (estrogenic and lipid disruption, eye development), making it advisable a tighter limitation on BPA exposure limits for both wildlife and human populations.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
Publisher's version
info:eu-repo/semantics/publishedVersion
format dataset
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/192701
url http://hdl.handle.net/10261/192701
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/FP7/320737
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2014-56777-R
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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