[Raw Data] Lipidomic effects of bisphenol A in zebrafish eleutheroembryos during the yolk sac absorption stage
Previous studies on the effects of bisphenol A (BPA; 4,4'-(propane-2,2-diyl)diphenol) in zebrafish identified lipid metabolism as particularly affected by BPA exposure. We perform here a lipidomic analysis in zebrafish eleutheroembryos exposed up to 8 mg/L of BPA from day 2 to day 6 post fertil...
| Autores: | , , , , , , |
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| Tipo de recurso: | conjunto de datos |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/192701 |
| Acceso en línea: | http://hdl.handle.net/10261/192701 |
| Access Level: | acceso abierto |
| Palabra clave: | Mass spectrometry Zebrafish Thin layer chromatography Yolk sac malabsorption Adverse outcome pathway Mode of action |
| Sumario: | Previous studies on the effects of bisphenol A (BPA; 4,4'-(propane-2,2-diyl)diphenol) in zebrafish identified lipid metabolism as particularly affected by BPA exposure. We perform here a lipidomic analysis in zebrafish eleutheroembryos exposed up to 8 mg/L of BPA from day 2 to day 6 post fertilization. Total lipids were analyzed by high-performance thin layer chromatography as wide-range preliminary screening. Selected conditions were used to obtain a high-quality lipid profile using ultra high-performance liquid chromatography/time-of-flight mass spectrometry. BPA specifically impaired the physiological consumption of triglycerides (TG), diglycerides (DG), phosphatidylcholines (PC) and phosphatidylinositols (PI), most of them yolk sac-related lipids. These effects seemed more related to a decrease in energy consumption in BPA exposed individuals than to an impairment of lipid transport from the yolk sac. Integration of these results with previous morphological, biochemical, transcriptomic, metabolomic and behavioral data suggests that changes on lipid metabolism first appeared at very low BPA concentrations, and that they propagated across different organization levels. The analysis also suggests that the disruption of different signalling pathways by BPA results in a combination of adverse outcomes (estrogenic and lipid disruption, eye development), making it advisable a tighter limitation on BPA exposure limits for both wildlife and human populations. |
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