Oligonucleotides complementary to c-myb messenger RNA inhibit growth and induce apoptosis in human Burkitt lymphoma cells

Joshi, S.S. (Shantaram S.)|||/items/585ea42f-51c4-4488-8ed4-13e9a0f5adea; Wu, AI.G. (AI G.)|||/items/e7c13550-8fb3-4464-9ef9-50d8534c266b; Verbik, D.J. (David J.)|||/items/e91ddc84-74a0-48c4-96a4-e0a973041baa; Martin-Algarra, S. (Salvador)|||/items/2fba8e8b-e087-4ab0-82e3-a4d157f1e0cc; Bishop, M.R. (Michael R.)|||/items/e3a08487-8583-406c-b9c9-c974f883f7e2; Pirruccello, S.J. (Samuel J.)|||/items/71e413df-5b6f-4c23-b232-7fbf5781e3d8; Iversen, P. L. (Patrick L.)|||/items/728d217d-c98a-4500-8f8e-7d970e177b0e; Jackson, J.D. (John D.)|||/items/e606fbfc-f4f6-4de3-9cd1-995e5cfe53b6; Kessinger, A. (Anne)|||/items/acbc9f8a-7b0c-4143-bc1d-b9677d504681; Graham-Sharp, J. (J.)|||/items/fed833a2-8a01-449c-b2c6-6c2653721d81

Oligonucleotides complementary to c-myb messenger RNA inhibit growth and induce apoptosis in human Burkitt lymphoma cells

A 24-mer (antisense) phosphorothioate oligonucleotide (ODN) corresponding to the codons 2-9 of the c-myb gene was evaluated for its effects on the growth of a human Burkitt lymphoma cell line (Raji) in vitro. Raji cells incubated with different concentrations of c-myb antisense ODN (5-15 M-g/ml) for...

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Detalhes bibliográficos
Autores:	Joshi, S.S. (Shantaram S.)\|\|\|/items/585ea42f-51c4-4488-8ed4-13e9a0f5adea, Wu, AI.G. (AI G.)\|\|\|/items/e7c13550-8fb3-4464-9ef9-50d8534c266b, Verbik, D.J. (David J.)\|\|\|/items/e91ddc84-74a0-48c4-96a4-e0a973041baa, Martin-Algarra, S. (Salvador)\|\|\|/items/2fba8e8b-e087-4ab0-82e3-a4d157f1e0cc, Bishop, M.R. (Michael R.)\|\|\|/items/e3a08487-8583-406c-b9c9-c974f883f7e2, Pirruccello, S.J. (Samuel J.)\|\|\|/items/71e413df-5b6f-4c23-b232-7fbf5781e3d8, Iversen, P. L. (Patrick L.)\|\|\|/items/728d217d-c98a-4500-8f8e-7d970e177b0e, Jackson, J.D. (John D.)\|\|\|/items/e606fbfc-f4f6-4de3-9cd1-995e5cfe53b6, Kessinger, A. (Anne)\|\|\|/items/acbc9f8a-7b0c-4143-bc1d-b9677d504681, Graham-Sharp, J. (J.)\|\|\|/items/fed833a2-8a01-449c-b2c6-6c2653721d81
Formato:	artículo
Fecha de publicación:	1996
País:	España
Recursos:	Universidad de Navarra
Repositorio:	Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:	inglés
OAI Identifier:	oai:dadun.unav.edu:10171/68915
Acesso em linha:	https://hdl.handle.net/10171/68915
Access Level:	acceso abierto
Palavra-chave:	Burkitt lymphoma Raji cells c-myb Antisense oligonucleotides Apoptosis

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spelling	Oligonucleotides complementary to c-myb messenger RNA inhibit growth and induce apoptosis in human Burkitt lymphoma cellsJoshi, S.S. (Shantaram S.)\|\|\|/items/585ea42f-51c4-4488-8ed4-13e9a0f5adeaWu, AI.G. (AI G.)\|\|\|/items/e7c13550-8fb3-4464-9ef9-50d8534c266bVerbik, D.J. (David J.)\|\|\|/items/e91ddc84-74a0-48c4-96a4-e0a973041baaMartin-Algarra, S. (Salvador)\|\|\|/items/2fba8e8b-e087-4ab0-82e3-a4d157f1e0ccBishop, M.R. (Michael R.)\|\|\|/items/e3a08487-8583-406c-b9c9-c974f883f7e2Pirruccello, S.J. (Samuel J.)\|\|\|/items/71e413df-5b6f-4c23-b232-7fbf5781e3d8Iversen, P. L. (Patrick L.)\|\|\|/items/728d217d-c98a-4500-8f8e-7d970e177b0eJackson, J.D. (John D.)\|\|\|/items/e606fbfc-f4f6-4de3-9cd1-995e5cfe53b6Kessinger, A. (Anne)\|\|\|/items/acbc9f8a-7b0c-4143-bc1d-b9677d504681Graham-Sharp, J. (J.)\|\|\|/items/fed833a2-8a01-449c-b2c6-6c2653721d81Burkitt lymphomaRaji cellsc-mybAntisense oligonucleotidesApoptosisA 24-mer (antisense) phosphorothioate oligonucleotide (ODN) corresponding to the codons 2-9 of the c-myb gene was evaluated for its effects on the growth of a human Burkitt lymphoma cell line (Raji) in vitro. Raji cells incubated with different concentrations of c-myb antisense ODN (5-15 M-g/ml) for 24-72 h showed a significant dosedependent decrease in growth. The same concentrations of control (sense) or scrambled c-myb phosphorothioate ODNs did not inhibit Raji cell growth. The c-myb antisense ODN, but not the control ODNs, significantly decreased c-myb mRNA levels in treated cells as determined by RT-PCR. Additionally, the c-myb antisense ODN induced apoptosis of Raji cells as demonstrated by i) flow cytometry to enumerate the A0 (apoptotic cell population) population of propidium iodide stained cells; ii) electron microscopy to evaluate the cell morphology; and iii) DNA fragmentation pattern. Thus, an antisense c-myb ODN causes significant growth inhibition of Burkitt lymphoma cells, and one mechanism of growth inhibition is the induction of apoptosis of the lymphoma cells. In addition, antisense c-myb ODN did not reduce CFUGM or BFU-e colony-forming ability of normal hematopoietic stem/progenitor cells. Because the inhibition is sequence-specific and Burkitt lymphoma cell selective, evaluation of the therapeutic effects of c-myb antisense ODN against Burkitt lymphoma is warranted.Spandidos PublicationsDadun. Depósito Académico Digital Universidad de Navarra20242024-02-0819961996-01-0119961996-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/68915reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/689152026-06-21T12:47:57Z
dc.title.none.fl_str_mv	Oligonucleotides complementary to c-myb messenger RNA inhibit growth and induce apoptosis in human Burkitt lymphoma cells
title	Oligonucleotides complementary to c-myb messenger RNA inhibit growth and induce apoptosis in human Burkitt lymphoma cells
spellingShingle	Oligonucleotides complementary to c-myb messenger RNA inhibit growth and induce apoptosis in human Burkitt lymphoma cells Joshi, S.S. (Shantaram S.)\|\|\|/items/585ea42f-51c4-4488-8ed4-13e9a0f5adea Burkitt lymphoma Raji cells c-myb Antisense oligonucleotides Apoptosis
title_short	Oligonucleotides complementary to c-myb messenger RNA inhibit growth and induce apoptosis in human Burkitt lymphoma cells
title_full	Oligonucleotides complementary to c-myb messenger RNA inhibit growth and induce apoptosis in human Burkitt lymphoma cells
title_fullStr	Oligonucleotides complementary to c-myb messenger RNA inhibit growth and induce apoptosis in human Burkitt lymphoma cells
title_full_unstemmed	Oligonucleotides complementary to c-myb messenger RNA inhibit growth and induce apoptosis in human Burkitt lymphoma cells
title_sort	Oligonucleotides complementary to c-myb messenger RNA inhibit growth and induce apoptosis in human Burkitt lymphoma cells
dc.creator.none.fl_str_mv	Joshi, S.S. (Shantaram S.)\|\|\|/items/585ea42f-51c4-4488-8ed4-13e9a0f5adea Wu, AI.G. (AI G.)\|\|\|/items/e7c13550-8fb3-4464-9ef9-50d8534c266b Verbik, D.J. (David J.)\|\|\|/items/e91ddc84-74a0-48c4-96a4-e0a973041baa Martin-Algarra, S. (Salvador)\|\|\|/items/2fba8e8b-e087-4ab0-82e3-a4d157f1e0cc Bishop, M.R. (Michael R.)\|\|\|/items/e3a08487-8583-406c-b9c9-c974f883f7e2 Pirruccello, S.J. (Samuel J.)\|\|\|/items/71e413df-5b6f-4c23-b232-7fbf5781e3d8 Iversen, P. L. (Patrick L.)\|\|\|/items/728d217d-c98a-4500-8f8e-7d970e177b0e Jackson, J.D. (John D.)\|\|\|/items/e606fbfc-f4f6-4de3-9cd1-995e5cfe53b6 Kessinger, A. (Anne)\|\|\|/items/acbc9f8a-7b0c-4143-bc1d-b9677d504681 Graham-Sharp, J. (J.)\|\|\|/items/fed833a2-8a01-449c-b2c6-6c2653721d81
author	Joshi, S.S. (Shantaram S.)\|\|\|/items/585ea42f-51c4-4488-8ed4-13e9a0f5adea
author_facet	Joshi, S.S. (Shantaram S.)\|\|\|/items/585ea42f-51c4-4488-8ed4-13e9a0f5adea Wu, AI.G. (AI G.)\|\|\|/items/e7c13550-8fb3-4464-9ef9-50d8534c266b Verbik, D.J. (David J.)\|\|\|/items/e91ddc84-74a0-48c4-96a4-e0a973041baa Martin-Algarra, S. (Salvador)\|\|\|/items/2fba8e8b-e087-4ab0-82e3-a4d157f1e0cc Bishop, M.R. (Michael R.)\|\|\|/items/e3a08487-8583-406c-b9c9-c974f883f7e2 Pirruccello, S.J. (Samuel J.)\|\|\|/items/71e413df-5b6f-4c23-b232-7fbf5781e3d8 Iversen, P. L. (Patrick L.)\|\|\|/items/728d217d-c98a-4500-8f8e-7d970e177b0e Jackson, J.D. (John D.)\|\|\|/items/e606fbfc-f4f6-4de3-9cd1-995e5cfe53b6 Kessinger, A. (Anne)\|\|\|/items/acbc9f8a-7b0c-4143-bc1d-b9677d504681 Graham-Sharp, J. (J.)\|\|\|/items/fed833a2-8a01-449c-b2c6-6c2653721d81
author_role	author
author2	Wu, AI.G. (AI G.)\|\|\|/items/e7c13550-8fb3-4464-9ef9-50d8534c266b Verbik, D.J. (David J.)\|\|\|/items/e91ddc84-74a0-48c4-96a4-e0a973041baa Martin-Algarra, S. (Salvador)\|\|\|/items/2fba8e8b-e087-4ab0-82e3-a4d157f1e0cc Bishop, M.R. (Michael R.)\|\|\|/items/e3a08487-8583-406c-b9c9-c974f883f7e2 Pirruccello, S.J. (Samuel J.)\|\|\|/items/71e413df-5b6f-4c23-b232-7fbf5781e3d8 Iversen, P. L. (Patrick L.)\|\|\|/items/728d217d-c98a-4500-8f8e-7d970e177b0e Jackson, J.D. (John D.)\|\|\|/items/e606fbfc-f4f6-4de3-9cd1-995e5cfe53b6 Kessinger, A. (Anne)\|\|\|/items/acbc9f8a-7b0c-4143-bc1d-b9677d504681 Graham-Sharp, J. (J.)\|\|\|/items/fed833a2-8a01-449c-b2c6-6c2653721d81
author2_role	author author author author author author author author author
dc.contributor.none.fl_str_mv	Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv	Burkitt lymphoma Raji cells c-myb Antisense oligonucleotides Apoptosis
topic	Burkitt lymphoma Raji cells c-myb Antisense oligonucleotides Apoptosis
description	A 24-mer (antisense) phosphorothioate oligonucleotide (ODN) corresponding to the codons 2-9 of the c-myb gene was evaluated for its effects on the growth of a human Burkitt lymphoma cell line (Raji) in vitro. Raji cells incubated with different concentrations of c-myb antisense ODN (5-15 M-g/ml) for 24-72 h showed a significant dosedependent decrease in growth. The same concentrations of control (sense) or scrambled c-myb phosphorothioate ODNs did not inhibit Raji cell growth. The c-myb antisense ODN, but not the control ODNs, significantly decreased c-myb mRNA levels in treated cells as determined by RT-PCR. Additionally, the c-myb antisense ODN induced apoptosis of Raji cells as demonstrated by i) flow cytometry to enumerate the A0 (apoptotic cell population) population of propidium iodide stained cells; ii) electron microscopy to evaluate the cell morphology; and iii) DNA fragmentation pattern. Thus, an antisense c-myb ODN causes significant growth inhibition of Burkitt lymphoma cells, and one mechanism of growth inhibition is the induction of apoptosis of the lymphoma cells. In addition, antisense c-myb ODN did not reduce CFUGM or BFU-e colony-forming ability of normal hematopoietic stem/progenitor cells. Because the inhibition is sequence-specific and Burkitt lymphoma cell selective, evaluation of the therapeutic effects of c-myb antisense ODN against Burkitt lymphoma is warranted.
publishDate	1996
dc.date.none.fl_str_mv	1996 1996-01-01 1996 1996-01-01 2024 2024-02-08
dc.type.none.fl_str_mv	journal article http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://hdl.handle.net/10171/68915
url	https://hdl.handle.net/10171/68915
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Spandidos Publications
publisher.none.fl_str_mv	Spandidos Publications
dc.source.none.fl_str_mv	reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra
instname_str	Universidad de Navarra
reponame_str	Dadun. Depósito Académico Digital de la Universidad de Navarra
collection	Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,300719

Oligonucleotides complementary to c-myb messenger RNA inhibit growth and induce apoptosis in human Burkitt lymphoma cells

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