Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production

This work studies, for the first time, the potential of a laccase from the endophyte fungus Hormonema sp. CECT-13092, compared to a laccase from the saprophyte fungus Trametes villosa, for delignification and detoxification of steam-exploded eucalypt to improve subsequent bioethanol production. Rega...

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Detalles Bibliográficos
Autores: García-Fuentevilla, Luisa, Eugenio, María E., Martín-Sampedro, Raquel, Ibarra, David
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/395730
Acceso en línea:http://hdl.handle.net/10261/395730
https://api.elsevier.com/content/abstract/scopus_id/86000454162
Access Level:acceso abierto
Palabra clave:Delignification
Detoxification
Endophytic fungi
Ethanol
Fermentation sugars
Hormonema sp. laccase
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spelling Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol productionGarcía-Fuentevilla, LuisaEugenio, María E.Martín-Sampedro, RaquelIbarra, DavidDelignificationDetoxificationEndophytic fungiEthanolFermentation sugarsHormonema sp. laccaseThis work studies, for the first time, the potential of a laccase from the endophyte fungus Hormonema sp. CECT-13092, compared to a laccase from the saprophyte fungus Trametes villosa, for delignification and detoxification of steam-exploded eucalypt to improve subsequent bioethanol production. Regarding laccase delignification, the use of Hormonema sp. and T. villosa laccases did not show evidence of delignification of steam-exploded material, and rather low glucose and xylose recoveries were obtained during saccharification assays of laccase-treated samples compared with their respective controls. With regard to laccase detoxification, the reduction of the total phenolic inhibitors content presents in steam-exploded material by both laccases (phenols removal of 47 % and 60 % by Hormonema sp. and T. villosa laccases, respectively), triggered the fermentation by Saccharomyces cerevisiae of laccase-treated samples when 0.2 g L-1 of inoculum was used during a simultaneous saccharification and fermentation (SSF) process. Moreover, when the inoculum was increased from 0.2 to 1.0 g L-1, both laccases shortened the yeast lag phase during the SSF process. Then, faster glucose consumption and ethanol production rates (ethanol productivity values of 0.25 and 0.28 g L-1 h-1 for T. villosa and Hormonema sp. laccases, respectively, compared to 0.02 g L-1 h-1 for control samples) were noticed. This fact proves the high potential of this new entophytic fungal laccase for bioethanol production enhancement, comparable to commercial laccases.The authors wish to thank the projects PID2022–141965OB-C21 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”, and TED 2021-132122B-C1, funded by MCIN/AEI/10.13039/501100011033 and by the “European UnionNextGenerationEU/PRTR”, to support this study. The project TEC-2024/BIO-27 (AgroSUSTEC-CM), funded by Comunidad de Madrid, is also grateful.Peer reviewedElsevier BVMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)European CommissionComunidad de MadridConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/395730https://api.elsevier.com/content/abstract/scopus_id/86000454162reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-141965OB-C21info:eu-repo/grantAgreement/AEI//TED2021-132122B-C1TEC-2024/BIO-27/SUSTEC-CMThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.nbt.2025.03.001https://doi.org/10.1016/j.nbt.2025.03.001Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3957302026-05-22T06:33:51Z
dc.title.none.fl_str_mv Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production
title Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production
spellingShingle Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production
García-Fuentevilla, Luisa
Delignification
Detoxification
Endophytic fungi
Ethanol
Fermentation sugars
Hormonema sp. laccase
title_short Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production
title_full Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production
title_fullStr Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production
title_full_unstemmed Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production
title_sort Applicability of a laccase from the eucalypt wood endophytic fungus Hormonema sp. CECT-13092 for advanced bioethanol production
dc.creator.none.fl_str_mv García-Fuentevilla, Luisa
Eugenio, María E.
Martín-Sampedro, Raquel
Ibarra, David
author García-Fuentevilla, Luisa
author_facet García-Fuentevilla, Luisa
Eugenio, María E.
Martín-Sampedro, Raquel
Ibarra, David
author_role author
author2 Eugenio, María E.
Martín-Sampedro, Raquel
Ibarra, David
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
European Commission
Comunidad de Madrid
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Delignification
Detoxification
Endophytic fungi
Ethanol
Fermentation sugars
Hormonema sp. laccase
topic Delignification
Detoxification
Endophytic fungi
Ethanol
Fermentation sugars
Hormonema sp. laccase
description This work studies, for the first time, the potential of a laccase from the endophyte fungus Hormonema sp. CECT-13092, compared to a laccase from the saprophyte fungus Trametes villosa, for delignification and detoxification of steam-exploded eucalypt to improve subsequent bioethanol production. Regarding laccase delignification, the use of Hormonema sp. and T. villosa laccases did not show evidence of delignification of steam-exploded material, and rather low glucose and xylose recoveries were obtained during saccharification assays of laccase-treated samples compared with their respective controls. With regard to laccase detoxification, the reduction of the total phenolic inhibitors content presents in steam-exploded material by both laccases (phenols removal of 47 % and 60 % by Hormonema sp. and T. villosa laccases, respectively), triggered the fermentation by Saccharomyces cerevisiae of laccase-treated samples when 0.2 g L-1 of inoculum was used during a simultaneous saccharification and fermentation (SSF) process. Moreover, when the inoculum was increased from 0.2 to 1.0 g L-1, both laccases shortened the yeast lag phase during the SSF process. Then, faster glucose consumption and ethanol production rates (ethanol productivity values of 0.25 and 0.28 g L-1 h-1 for T. villosa and Hormonema sp. laccases, respectively, compared to 0.02 g L-1 h-1 for control samples) were noticed. This fact proves the high potential of this new entophytic fungal laccase for bioethanol production enhancement, comparable to commercial laccases.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/395730
https://api.elsevier.com/content/abstract/scopus_id/86000454162
url http://hdl.handle.net/10261/395730
https://api.elsevier.com/content/abstract/scopus_id/86000454162
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-141965OB-C21
info:eu-repo/grantAgreement/AEI//TED2021-132122B-C1
TEC-2024/BIO-27/SUSTEC-CM
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.nbt.2025.03.001
https://doi.org/10.1016/j.nbt.2025.03.001
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier BV
publisher.none.fl_str_mv Elsevier BV
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
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