Optimizing delignification and pitch removal by treating eucalypt pulp with Myceliophthora laccase and a phenolic mediator

This work shows the ability of two natural phenols, namely syringaldehyde and methyl syringate, to act as mediators of recombinant Myceliophthora thermophila laccase (MtL) in eucalypt-pulp delignification. After alkaline peroxide extraction, the properties of the enzymatically-treated pulps improved...

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Detalhes bibliográficos
Autores: Rico Campos, Alejandro, Babot, Esteban Daniel, Rencoret, Jorge, Río Andrade, José Carlos del, Martínez, Ángel T., Gutiérrez Suárez, Ana
Formato: otro
Fecha de publicación:2012
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/86330
Acesso em linha:http://hdl.handle.net/10261/86330
Access Level:acceso abierto
Palavra-chave:Delignification
Pitch
Eucalyptus
Pulp
Laccases
Myceliophthora thermophila
Descrição
Resumo:This work shows the ability of two natural phenols, namely syringaldehyde and methyl syringate, to act as mediators of recombinant Myceliophthora thermophila laccase (MtL) in eucalypt-pulp delignification. After alkaline peroxide extraction, the properties of the enzymatically-treated pulps improved with respect to the control. Likewise, removal of the main lipophilic extractives present in eucalypt pulp, such as free and conjugated sterols, was observed after the above laccase-mediator treatments. In the same study, the doses of both MtL and methyl syringate were reduced, and results compatible with industrial implementation were obtained, taking also advantage from the high-yield production of the recombinant enzyme that enables its commercial availability.