Cofactors of wheat-dependent exercise-induced anaphylaxis do not increase highly individual gliadin absorption in healthy volunteers

[Background] In wheat-dependent exercise-induced anaphylaxis (WDEIA), cofactors such as exercise, acetylsalicylic acid (ASA), alcohol or unfavorable climatic conditions are required to elicit a reaction to wheat products. The mechanism of action of these cofactors is unknown, but an increase of glia...

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Autores: Scherf, Katharina A., Lindenau, Ann-Christin, Valentini, Luzia, Collado, María Carmen, García Mantrana, Izaskun, Christensen, Morten, Tomsitz, Dirk, Kugler, Claudia, Biedermann, Tilo, Brockow, Knut
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/178966
Acceso en línea:http://hdl.handle.net/10261/178966
Access Level:acceso abierto
Palabra clave:Cofactor
Gliadin
Gluten
Gut microbiota
Intestinal permeability
Wheat allergy
Wheat-dependent exercise induced anaphylaxis (WDEIA)
Zonulin
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spelling Cofactors of wheat-dependent exercise-induced anaphylaxis do not increase highly individual gliadin absorption in healthy volunteersScherf, Katharina A.Lindenau, Ann-ChristinValentini, LuziaCollado, María CarmenGarcía Mantrana, IzaskunChristensen, MortenTomsitz, DirkKugler, ClaudiaBiedermann, TiloBrockow, KnutCofactorGliadinGlutenGut microbiotaIntestinal permeabilityWheat allergyWheat-dependent exercise induced anaphylaxis (WDEIA)Zonulin[Background] In wheat-dependent exercise-induced anaphylaxis (WDEIA), cofactors such as exercise, acetylsalicylic acid (ASA), alcohol or unfavorable climatic conditions are required to elicit a reaction to wheat products. The mechanism of action of these cofactors is unknown, but an increase of gliadin absorption has been speculated. Our objectives were to study gliadin absorption with and without cofactors and to correlate plasma gliadin levels with factors influencing protein absorption in healthy volunteers.[Methods] Twelve healthy probands (six males, six females; aged 20–56 years) ingested 32 g of gluten without any cofactor or in combination with cofactors aerobic and anaerobic exercise, ASA, alcohol and pantoprazole. Gliadin serum levels were measured up to 120 min afterwards and the intestinal barrier function protein zonulin in stool was collected before and after the procedure; both were measured by ELISA. Stool microbiota profile was obtained by 16S gene sequencing.[Results] Within 15 min after gluten intake, gliadin concentrations in blood serum increased from baseline in all subjects reaching highly variable peak levels after 15–90 min. Addition of cofactors did not lead to substantially higher gliadin levels, although variability of levels was higher with differences between individuals (p < 0.001) and increased levels at later time points. Zonulin levels in stool were associated neither with addition of cofactors nor with peak gliadin concentrations. There were no differences in gut microbiota between the different interventions, although the composition of microbiota (p < 0.001) and the redundancy discriminant analysis (p < 0.007) differed in probands with low versus high stool zonulin levels.[Conclusion] The adsorption of gliadin in the gut in healthy volunteers is less dependent on cofactors than has been hypothesized. Patients with WDEIA may have a predisposition needed for the additional effect of cofactors, e.g., hyperresponsive or damaged intestinal epithelium. Alternatively, other mechanisms, such as cofactor-induced blood flow redistribution, increased activity of tissue transglutaminase, or increases in plasma osmolality and acidosis inducing basophil and mast cell histamine release may play the major role in WDEIA.This work was supported by the German Research Foundation (DFG) and the Technical University of Munich (TUM) in the framework of the Open Access Publishing Program.Peer reviewedBioMed CentralGerman Research FoundationTechnical University of MunichConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/178966reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1186/s13601-019-0260-0Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1789662026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cofactors of wheat-dependent exercise-induced anaphylaxis do not increase highly individual gliadin absorption in healthy volunteers
title Cofactors of wheat-dependent exercise-induced anaphylaxis do not increase highly individual gliadin absorption in healthy volunteers
spellingShingle Cofactors of wheat-dependent exercise-induced anaphylaxis do not increase highly individual gliadin absorption in healthy volunteers
Scherf, Katharina A.
Cofactor
Gliadin
Gluten
Gut microbiota
Intestinal permeability
Wheat allergy
Wheat-dependent exercise induced anaphylaxis (WDEIA)
Zonulin
title_short Cofactors of wheat-dependent exercise-induced anaphylaxis do not increase highly individual gliadin absorption in healthy volunteers
title_full Cofactors of wheat-dependent exercise-induced anaphylaxis do not increase highly individual gliadin absorption in healthy volunteers
title_fullStr Cofactors of wheat-dependent exercise-induced anaphylaxis do not increase highly individual gliadin absorption in healthy volunteers
title_full_unstemmed Cofactors of wheat-dependent exercise-induced anaphylaxis do not increase highly individual gliadin absorption in healthy volunteers
title_sort Cofactors of wheat-dependent exercise-induced anaphylaxis do not increase highly individual gliadin absorption in healthy volunteers
dc.creator.none.fl_str_mv Scherf, Katharina A.
Lindenau, Ann-Christin
Valentini, Luzia
Collado, María Carmen
García Mantrana, Izaskun
Christensen, Morten
Tomsitz, Dirk
Kugler, Claudia
Biedermann, Tilo
Brockow, Knut
author Scherf, Katharina A.
author_facet Scherf, Katharina A.
Lindenau, Ann-Christin
Valentini, Luzia
Collado, María Carmen
García Mantrana, Izaskun
Christensen, Morten
Tomsitz, Dirk
Kugler, Claudia
Biedermann, Tilo
Brockow, Knut
author_role author
author2 Lindenau, Ann-Christin
Valentini, Luzia
Collado, María Carmen
García Mantrana, Izaskun
Christensen, Morten
Tomsitz, Dirk
Kugler, Claudia
Biedermann, Tilo
Brockow, Knut
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv German Research Foundation
Technical University of Munich
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cofactor
Gliadin
Gluten
Gut microbiota
Intestinal permeability
Wheat allergy
Wheat-dependent exercise induced anaphylaxis (WDEIA)
Zonulin
topic Cofactor
Gliadin
Gluten
Gut microbiota
Intestinal permeability
Wheat allergy
Wheat-dependent exercise induced anaphylaxis (WDEIA)
Zonulin
description [Background] In wheat-dependent exercise-induced anaphylaxis (WDEIA), cofactors such as exercise, acetylsalicylic acid (ASA), alcohol or unfavorable climatic conditions are required to elicit a reaction to wheat products. The mechanism of action of these cofactors is unknown, but an increase of gliadin absorption has been speculated. Our objectives were to study gliadin absorption with and without cofactors and to correlate plasma gliadin levels with factors influencing protein absorption in healthy volunteers.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/178966
url http://hdl.handle.net/10261/178966
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1186/s13601-019-0260-0
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425319855783936
score 15.811543

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