New insights on Arabidopsis thaliana root adaption to ammonium nutrition by the use of a quantitative proteomic approach

Nitrogen is an essential element for plant nutrition. Nitrate and ammonium are the two major inorganic nitrogen forms available for plant growth. Plant preference for one or the other form depends on the interplay between plant genetic background and environmental variables. Ammonium-based fertiliza...

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Autores: Coleto, Inmaculada, Vega-Mas, Izargi, Glauser, Gaëtan, González Moro, María Begoña, Marino, Daniel, Ariz Arnedo, Idoia
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/53515
Acceso en línea:https://hdl.handle.net/2454/53515
Access Level:acceso abierto
Palabra clave:Ammonium
Arabidopsis thaliana
Carbon metabolism
Nitrogen metabolism
Nitrate
Proteomics
Root
Secondary metabolism
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spelling New insights on Arabidopsis thaliana root adaption to ammonium nutrition by the use of a quantitative proteomic approachColeto, InmaculadaVega-Mas, IzargiGlauser, GaëtanGonzález Moro, María BegoñaMarino, DanielAriz Arnedo, IdoiaAmmoniumArabidopsis thalianaCarbon metabolismNitrogen metabolismNitrateProteomicsRootSecondary metabolismNitrogen is an essential element for plant nutrition. Nitrate and ammonium are the two major inorganic nitrogen forms available for plant growth. Plant preference for one or the other form depends on the interplay between plant genetic background and environmental variables. Ammonium-based fertilization has been shown less environmentally harmful compared to nitrate fertilization, because of reducing, among others, nitrate leaching and nitrous oxide emissions. However, ammonium nutrition may become a stressful situation for a wide range of plant species when the ion is present at high concentrations. Although studied for long time, there is still an important lack of knowledge to explain plant tolerance or sensitivity towards ammonium nutrition. In this context, we performed a comparative proteomic study in roots of Arabidopsis thaliana plants grown under exclusive ammonium or nitrate supply. We identified and quantified 68 proteins with differential abundance between both conditions. These proteins revealed new potential important players on root response to ammonium nutrition, such as H+-consuming metabolic pathways to regulate pH homeostasis and specific secondary metabolic pathways like brassinosteroid and glucosinolate biosynthetic pathways.This research was financially supported by the Basque Government (IT932-16), the Spanish Ministry of Economy and Competitiveness (BIO2014-56271-R and BIO2017-84035-R co-funded by FEDER). I.A. was supported by a Juan de la Cierva postdoctoral contract (IJCI-2015-26002) from the Spanish Ministry of Economy and Competitiveness. I.C. was supported by a postdoctoral fellowship from the University of the Basque Country UPV/EHU.MDPICienciasZientziakInstitute for Multidisciplinary Research in Applied Biology - IMAB2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/ziphttps://hdl.handle.net/2454/53515reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglésinfo:eu-repo/grantAgreement/MINECO//BIO2014-56271-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-84035-R© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/535152026-06-17T12:41:47Z
dc.title.none.fl_str_mv New insights on Arabidopsis thaliana root adaption to ammonium nutrition by the use of a quantitative proteomic approach
title New insights on Arabidopsis thaliana root adaption to ammonium nutrition by the use of a quantitative proteomic approach
spellingShingle New insights on Arabidopsis thaliana root adaption to ammonium nutrition by the use of a quantitative proteomic approach
Coleto, Inmaculada
Ammonium
Arabidopsis thaliana
Carbon metabolism
Nitrogen metabolism
Nitrate
Proteomics
Root
Secondary metabolism
title_short New insights on Arabidopsis thaliana root adaption to ammonium nutrition by the use of a quantitative proteomic approach
title_full New insights on Arabidopsis thaliana root adaption to ammonium nutrition by the use of a quantitative proteomic approach
title_fullStr New insights on Arabidopsis thaliana root adaption to ammonium nutrition by the use of a quantitative proteomic approach
title_full_unstemmed New insights on Arabidopsis thaliana root adaption to ammonium nutrition by the use of a quantitative proteomic approach
title_sort New insights on Arabidopsis thaliana root adaption to ammonium nutrition by the use of a quantitative proteomic approach
dc.creator.none.fl_str_mv Coleto, Inmaculada
Vega-Mas, Izargi
Glauser, Gaëtan
González Moro, María Begoña
Marino, Daniel
Ariz Arnedo, Idoia
author Coleto, Inmaculada
author_facet Coleto, Inmaculada
Vega-Mas, Izargi
Glauser, Gaëtan
González Moro, María Begoña
Marino, Daniel
Ariz Arnedo, Idoia
author_role author
author2 Vega-Mas, Izargi
Glauser, Gaëtan
González Moro, María Begoña
Marino, Daniel
Ariz Arnedo, Idoia
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ciencias
Zientziak
Institute for Multidisciplinary Research in Applied Biology - IMAB
dc.subject.none.fl_str_mv Ammonium
Arabidopsis thaliana
Carbon metabolism
Nitrogen metabolism
Nitrate
Proteomics
Root
Secondary metabolism
topic Ammonium
Arabidopsis thaliana
Carbon metabolism
Nitrogen metabolism
Nitrate
Proteomics
Root
Secondary metabolism
description Nitrogen is an essential element for plant nutrition. Nitrate and ammonium are the two major inorganic nitrogen forms available for plant growth. Plant preference for one or the other form depends on the interplay between plant genetic background and environmental variables. Ammonium-based fertilization has been shown less environmentally harmful compared to nitrate fertilization, because of reducing, among others, nitrate leaching and nitrous oxide emissions. However, ammonium nutrition may become a stressful situation for a wide range of plant species when the ion is present at high concentrations. Although studied for long time, there is still an important lack of knowledge to explain plant tolerance or sensitivity towards ammonium nutrition. In this context, we performed a comparative proteomic study in roots of Arabidopsis thaliana plants grown under exclusive ammonium or nitrate supply. We identified and quantified 68 proteins with differential abundance between both conditions. These proteins revealed new potential important players on root response to ammonium nutrition, such as H+-consuming metabolic pathways to regulate pH homeostasis and specific secondary metabolic pathways like brassinosteroid and glucosinolate biosynthetic pathways.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/53515
url https://hdl.handle.net/2454/53515
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO//BIO2014-56271-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-84035-R
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/zip
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
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