Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops

[EN]The legume nodules are a rich source not only of rhizobia but also of endophytic bacteria exhibiting plant growth-promoting mechanisms with potential as plant biostimulants. In this work we analyzed the genomes of Phyllobacterium endophyticum PEPV15 and Rhizobium laguerreae PEPV16 strains, both...

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Detalhes bibliográficos
Autores: Flores Félix, José David, Velázquez Pérez, María Encarnación, Martínez Molina, Eustoquio, González Andrés, Fernando, Squartini, Andrea, Rivas González, Raúl
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Recursos:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/154253
Acesso em linha:http://hdl.handle.net/10366/154253
Access Level:acceso abierto
Palavra-chave:Phyllobacterium
Rhizobium
Carrot
Lettuce
Bacterial endophytes
Plant growth promotion
PGPB
Genomics
Microbial biostimulants
Field assays
Food Microbiology
Microbiology
Environmental Microbiology
2414 Microbiología
microbiología
microbiología de los alimentos
microbiología ambiental
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repository_id_str
spelling Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable cropsFlores Félix, José DavidVelázquez Pérez, María EncarnaciónMartínez Molina, EustoquioGonzález Andrés, FernandoSquartini, AndreaRivas González, RaúlPhyllobacteriumRhizobiumCarrotLettuceBacterial endophytesPlant growth promotionPGPBGenomicsMicrobial biostimulantsField assaysFood MicrobiologyMicrobiologyEnvironmental Microbiology2414 Microbiologíamicrobiologíamicrobiología de los alimentosmicrobiología ambiental[EN]The legume nodules are a rich source not only of rhizobia but also of endophytic bacteria exhibiting plant growth-promoting mechanisms with potential as plant biostimulants. In this work we analyzed the genomes of Phyllobacterium endophyticum PEPV15 and Rhizobium laguerreae PEPV16 strains, both isolated from Phaseolus vulgaris nodules. In silico analysis showed that the genomes of these two strains contain genes related to N-acyl-homoserine lactone (AHL) and cellulose biosynthesis, involved in quorum sensing and biofilm formation, which are essential for plant colonization. Several genes involved in plant growth promotion such as those related to phosphate solubilization, indole acetic acid production, siderophore biosynthesis and nitrogen fixation were also located in both genomes. When strains PEPV15 and PEPV16 were inoculated in lettuce and carrot in field assays, we found that both significantly increased the yield of lettuce shoots and carrot roots by more than 20% and 10%, respectively. The results of this work confirmed that the genome mining of genes involved in plant colonization and growth promotion is a good strategy for predicting the potential of bacterial strains as crops inoculants, opening new horizons for the selection of bacterial strains with which to design new, effective bacteria-based plant biostimulants.MDPI202420242021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/154253reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésSA183A11-2Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1542532026-06-07T06:28:51Z
dc.title.none.fl_str_mv Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops
title Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops
spellingShingle Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops
Flores Félix, José David
Phyllobacterium
Rhizobium
Carrot
Lettuce
Bacterial endophytes
Plant growth promotion
PGPB
Genomics
Microbial biostimulants
Field assays
Food Microbiology
Microbiology
Environmental Microbiology
2414 Microbiología
microbiología
microbiología de los alimentos
microbiología ambiental
title_short Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops
title_full Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops
title_fullStr Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops
title_full_unstemmed Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops
title_sort Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops
dc.creator.none.fl_str_mv Flores Félix, José David
Velázquez Pérez, María Encarnación
Martínez Molina, Eustoquio
González Andrés, Fernando
Squartini, Andrea
Rivas González, Raúl
author Flores Félix, José David
author_facet Flores Félix, José David
Velázquez Pérez, María Encarnación
Martínez Molina, Eustoquio
González Andrés, Fernando
Squartini, Andrea
Rivas González, Raúl
author_role author
author2 Velázquez Pérez, María Encarnación
Martínez Molina, Eustoquio
González Andrés, Fernando
Squartini, Andrea
Rivas González, Raúl
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Phyllobacterium
Rhizobium
Carrot
Lettuce
Bacterial endophytes
Plant growth promotion
PGPB
Genomics
Microbial biostimulants
Field assays
Food Microbiology
Microbiology
Environmental Microbiology
2414 Microbiología
microbiología
microbiología de los alimentos
microbiología ambiental
topic Phyllobacterium
Rhizobium
Carrot
Lettuce
Bacterial endophytes
Plant growth promotion
PGPB
Genomics
Microbial biostimulants
Field assays
Food Microbiology
Microbiology
Environmental Microbiology
2414 Microbiología
microbiología
microbiología de los alimentos
microbiología ambiental
description [EN]The legume nodules are a rich source not only of rhizobia but also of endophytic bacteria exhibiting plant growth-promoting mechanisms with potential as plant biostimulants. In this work we analyzed the genomes of Phyllobacterium endophyticum PEPV15 and Rhizobium laguerreae PEPV16 strains, both isolated from Phaseolus vulgaris nodules. In silico analysis showed that the genomes of these two strains contain genes related to N-acyl-homoserine lactone (AHL) and cellulose biosynthesis, involved in quorum sensing and biofilm formation, which are essential for plant colonization. Several genes involved in plant growth promotion such as those related to phosphate solubilization, indole acetic acid production, siderophore biosynthesis and nitrogen fixation were also located in both genomes. When strains PEPV15 and PEPV16 were inoculated in lettuce and carrot in field assays, we found that both significantly increased the yield of lettuce shoots and carrot roots by more than 20% and 10%, respectively. The results of this work confirmed that the genome mining of genes involved in plant colonization and growth promotion is a good strategy for predicting the potential of bacterial strains as crops inoculants, opening new horizons for the selection of bacterial strains with which to design new, effective bacteria-based plant biostimulants.
publishDate 2021
dc.date.none.fl_str_mv 2021
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/154253
url http://hdl.handle.net/10366/154253
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv SA183A11-2
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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