Structural and Nonstructural Viral Proteins Are Targets of T-Helper Immune Response against Human Respiratory Syncytial Virus

Proper antiviral humoral and cellular immune responses require previous recognition of viral antigenic peptides that are bound to HLA class II molecules, which are exposed on the surface of antigen-presenting cells. The helper immune response is critical for the control and the clearance of human re...

Descripción completa

Detalles Bibliográficos
Autores: Lorente, Elena, Barriga, Alejandro, Barnea, Eilon, Mir-Gerrero, Carmen, Gebe, John A, Admon, Arie, Lopez, Daniel
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/9093
Acceso en línea:http://hdl.handle.net/20.500.12105/9093
Access Level:acceso abierto
Palabra clave:Animals
Antigen Presentation
Crystallography, X-Ray
Histocompatibility Antigens Class II
Humans
Immunity, Cellular
B-Lymphocyte Subsets
Mice
Peptides
Proteomics
Respiratory Syncytial Virus, Human
T-Lymphocytes, Helper-Inducer
Viral Nonstructural Proteins
Viral Structural Proteins
id ES_fa9a67345223a1c4f597dcd4182a26d5
oai_identifier_str oai:repisalud.isciii.es:20.500.12105/9093
network_acronym_str ES
network_name_str España
repository_id_str
spelling Structural and Nonstructural Viral Proteins Are Targets of T-Helper Immune Response against Human Respiratory Syncytial VirusLorente, ElenaBarriga, AlejandroBarnea, EilonMir-Gerrero, CarmenGebe, John AAdmon, ArieLopez, DanielAnimalsAntigen PresentationCrystallography, X-RayHistocompatibility Antigens Class IIHumansImmunity, CellularB-Lymphocyte SubsetsMicePeptidesProteomicsRespiratory Syncytial Virus, HumanT-Lymphocytes, Helper-InducerViral Nonstructural ProteinsViral Structural ProteinsProper antiviral humoral and cellular immune responses require previous recognition of viral antigenic peptides that are bound to HLA class II molecules, which are exposed on the surface of antigen-presenting cells. The helper immune response is critical for the control and the clearance of human respiratory syncytial virus (HRSV) infection, a virus with severe health risk in infected pediatric, immunocompromised, and elderly populations. In this study, using a mass spectrometry analysis of complex HLA class II-bound peptide pools that were isolated from large amounts of HRSV-infected cells, 19 naturally processed HLA-DR ligands, most of them included in a complex nested set of peptides, were identified. Both the immunoprevalence and the immunodominance of the HLA class II response to HRSV were focused on one nonstructural (NS1) and two structural (matrix and mainly fusion) proteins of the infective virus. These findings have clear implications for analysis of the helper immune response as well as for antiviral vaccine design.American Society for Biochemistry and Molecular Biology (ASBMB)Ministerio de Economía y Competitividad (España)20202020-02-1420162016-01-0120162016-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/9093reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)InglésengES BIO2011–25636 Not availableES ISF 916 05open accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/90932026-06-12T12:43:37Z
dc.title.none.fl_str_mv Structural and Nonstructural Viral Proteins Are Targets of T-Helper Immune Response against Human Respiratory Syncytial Virus
title Structural and Nonstructural Viral Proteins Are Targets of T-Helper Immune Response against Human Respiratory Syncytial Virus
spellingShingle Structural and Nonstructural Viral Proteins Are Targets of T-Helper Immune Response against Human Respiratory Syncytial Virus
Lorente, Elena
Animals
Antigen Presentation
Crystallography, X-Ray
Histocompatibility Antigens Class II
Humans
Immunity, Cellular
B-Lymphocyte Subsets
Mice
Peptides
Proteomics
Respiratory Syncytial Virus, Human
T-Lymphocytes, Helper-Inducer
Viral Nonstructural Proteins
Viral Structural Proteins
title_short Structural and Nonstructural Viral Proteins Are Targets of T-Helper Immune Response against Human Respiratory Syncytial Virus
title_full Structural and Nonstructural Viral Proteins Are Targets of T-Helper Immune Response against Human Respiratory Syncytial Virus
title_fullStr Structural and Nonstructural Viral Proteins Are Targets of T-Helper Immune Response against Human Respiratory Syncytial Virus
title_full_unstemmed Structural and Nonstructural Viral Proteins Are Targets of T-Helper Immune Response against Human Respiratory Syncytial Virus
title_sort Structural and Nonstructural Viral Proteins Are Targets of T-Helper Immune Response against Human Respiratory Syncytial Virus
dc.creator.none.fl_str_mv Lorente, Elena
Barriga, Alejandro
Barnea, Eilon
Mir-Gerrero, Carmen
Gebe, John A
Admon, Arie
Lopez, Daniel
author Lorente, Elena
author_facet Lorente, Elena
Barriga, Alejandro
Barnea, Eilon
Mir-Gerrero, Carmen
Gebe, John A
Admon, Arie
Lopez, Daniel
author_role author
author2 Barriga, Alejandro
Barnea, Eilon
Mir-Gerrero, Carmen
Gebe, John A
Admon, Arie
Lopez, Daniel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
dc.subject.none.fl_str_mv Animals
Antigen Presentation
Crystallography, X-Ray
Histocompatibility Antigens Class II
Humans
Immunity, Cellular
B-Lymphocyte Subsets
Mice
Peptides
Proteomics
Respiratory Syncytial Virus, Human
T-Lymphocytes, Helper-Inducer
Viral Nonstructural Proteins
Viral Structural Proteins
topic Animals
Antigen Presentation
Crystallography, X-Ray
Histocompatibility Antigens Class II
Humans
Immunity, Cellular
B-Lymphocyte Subsets
Mice
Peptides
Proteomics
Respiratory Syncytial Virus, Human
T-Lymphocytes, Helper-Inducer
Viral Nonstructural Proteins
Viral Structural Proteins
description Proper antiviral humoral and cellular immune responses require previous recognition of viral antigenic peptides that are bound to HLA class II molecules, which are exposed on the surface of antigen-presenting cells. The helper immune response is critical for the control and the clearance of human respiratory syncytial virus (HRSV) infection, a virus with severe health risk in infected pediatric, immunocompromised, and elderly populations. In this study, using a mass spectrometry analysis of complex HLA class II-bound peptide pools that were isolated from large amounts of HRSV-infected cells, 19 naturally processed HLA-DR ligands, most of them included in a complex nested set of peptides, were identified. Both the immunoprevalence and the immunodominance of the HLA class II response to HRSV were focused on one nonstructural (NS1) and two structural (matrix and mainly fusion) proteins of the infective virus. These findings have clear implications for analysis of the helper immune response as well as for antiviral vaccine design.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01
2016
2016-01-01
2020
2020-02-14
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/9093
url http://hdl.handle.net/20.500.12105/9093
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv ES BIO2011–25636 Not available
ES ISF 916 05
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology (ASBMB)
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology (ASBMB)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425226034446337
score 15.30478

Similares en LA Referencia