Proteomic analysis of the esophageal epithelium reveals key features of eosinophilic esophagitis pathophysiology

Molina Jiménez, Francisca; Ugalde Triviño, Lola; Arias González, Laura; Relaño Rupérez, Carlos; Casabona, Sergio; Pérez Fernández, María Teresa; Martín Domínguez, Verónica; Fernández Pacheco, Jennifer; Laserna Mendieta, Emilio José; Muñoz Hernández, Patricia; Arias Arias, Ángel; Cano, Ainara; Muñoz, Javier; Lucendo, Alfredo J.; Santander, Cecilio; Majano, Pedro; Majano Rodríguez, Pedro Lorenzo

Proteomic analysis of the esophageal epithelium reveals key features of eosinophilic esophagitis pathophysiology

Background Eosinophilic esophagitis (EoE) is a chronic non-IgE-mediated allergic disease of the esophagus. An unbiased proteomics approach was performed to investigate pathophysiological changes in esophageal epithelium. Additionally, an RNAseq-based transcriptomic analysis in paired samples was als...

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Detalhes bibliográficos
Autores:	Molina Jiménez, Francisca, Ugalde Triviño, Lola, Arias González, Laura, Relaño Rupérez, Carlos, Casabona, Sergio, Pérez Fernández, María Teresa, Martín Domínguez, Verónica, Fernández Pacheco, Jennifer, Laserna Mendieta, Emilio José, Muñoz Hernández, Patricia, Arias Arias, Ángel, Cano, Ainara, Muñoz, Javier, Lucendo, Alfredo J., Santander, Cecilio, Majano, Pedro, Majano Rodríguez, Pedro Lorenzo
Formato:	artículo
Fecha de publicación:	2023
País:	España
Recursos:	Universidad Complutense de Madrid (UCM)
Repositorio:	Docta Complutense
Idioma:	inglés
OAI Identifier:	oai:docta.ucm.es:20.500.14352/102911
Acesso em linha:	https://hdl.handle.net/20.500.14352/102911
Access Level:	acceso abierto
Palavra-chave:	576 616-092 Basic mechanisms Eosinophilic esophagitis Epithelium Proteomic Biología celular (Biología) Gastroenterología y hepatología 2407 Biología Celular 3205.03 Gastroenterología 3207 Patología

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spelling	Proteomic analysis of the esophageal epithelium reveals key features of eosinophilic esophagitis pathophysiologyMolina Jiménez, FranciscaUgalde Triviño, LolaArias González, LauraRelaño Rupérez, CarlosCasabona, SergioPérez Fernández, María TeresaMartín Domínguez, VerónicaFernández Pacheco, JenniferLaserna Mendieta, Emilio JoséMuñoz Hernández, PatriciaArias Arias, ÁngelCano, AinaraMuñoz, JavierLucendo, Alfredo J.Santander, CecilioMajano, PedroMajano Rodríguez, Pedro Lorenzo576616-092Basic mechanismsEosinophilic esophagitisEpitheliumProteomicBiología celular (Biología)Gastroenterología y hepatología2407 Biología Celular3205.03 Gastroenterología3207 PatologíaBackground Eosinophilic esophagitis (EoE) is a chronic non-IgE-mediated allergic disease of the esophagus. An unbiased proteomics approach was performed to investigate pathophysiological changes in esophageal epithelium. Additionally, an RNAseq-based transcriptomic analysis in paired samples was also carried out. Methods Total proteins were purified from esophageal endoscopic biopsies in a cohort of adult EoE patients (n = 25) and healthy esophagus controls (n = 10). Differentially accumulated (DA) proteins in EoE patients compared to control tissues were characterized to identify altered biological processes and signaling pathways. Results were also compared with a quantitative proteome dataset of the human esophageal mucosa. Next, results were contrasted with those obtained after RNAseq analysis in paired samples. Finally, we matched up protein expression with two EoE-specific mRNA panels (EDP and Eso-EoE panel). Results A total of 1667 proteins were identified, of which 363 were DA in EoE. RNA sequencing in paired samples identified 1993 differentially expressed (DE) genes. Total RNA and protein levels positively correlated, especially in DE mRNA-proteins pairs. Pathway analysis of these proteins in EoE showed alterations in immune and inflammatory responses for the upregulated proteins, and in epithelial differentiation, cornification and keratinization in those downregulated. Interestingly, a set of DA proteins, including eosinophil-related and secreted proteins, were not detected at the mRNA level. Protein expression positively correlated with EDP and Eso-EoE, and corresponded with the most abundant proteins of the human esophageal proteome. Conclusions We unraveled for the first time key proteomic features involved in EoE pathogenesis. An integrative analysis of transcriptomic and proteomic datasets provides a deeper insight than transcriptomic alone into understanding complex disease mechanisms.WileyLucendo, Alfredo J.Santander, CecilioMajano Rodríguez, Pedro LorenzoUniversidad Complutense de Madrid20232023-10-0120232023-10-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/102911reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1029112026-06-02T12:44:21Z
dc.title.none.fl_str_mv	Proteomic analysis of the esophageal epithelium reveals key features of eosinophilic esophagitis pathophysiology
title	Proteomic analysis of the esophageal epithelium reveals key features of eosinophilic esophagitis pathophysiology
spellingShingle	Proteomic analysis of the esophageal epithelium reveals key features of eosinophilic esophagitis pathophysiology Molina Jiménez, Francisca 576 616-092 Basic mechanisms Eosinophilic esophagitis Epithelium Proteomic Biología celular (Biología) Gastroenterología y hepatología 2407 Biología Celular 3205.03 Gastroenterología 3207 Patología
title_short	Proteomic analysis of the esophageal epithelium reveals key features of eosinophilic esophagitis pathophysiology
title_full	Proteomic analysis of the esophageal epithelium reveals key features of eosinophilic esophagitis pathophysiology
title_fullStr	Proteomic analysis of the esophageal epithelium reveals key features of eosinophilic esophagitis pathophysiology
title_full_unstemmed	Proteomic analysis of the esophageal epithelium reveals key features of eosinophilic esophagitis pathophysiology
title_sort	Proteomic analysis of the esophageal epithelium reveals key features of eosinophilic esophagitis pathophysiology
dc.creator.none.fl_str_mv	Molina Jiménez, Francisca Ugalde Triviño, Lola Arias González, Laura Relaño Rupérez, Carlos Casabona, Sergio Pérez Fernández, María Teresa Martín Domínguez, Verónica Fernández Pacheco, Jennifer Laserna Mendieta, Emilio José Muñoz Hernández, Patricia Arias Arias, Ángel Cano, Ainara Muñoz, Javier Lucendo, Alfredo J. Santander, Cecilio Majano, Pedro Majano Rodríguez, Pedro Lorenzo
author	Molina Jiménez, Francisca
author_facet	Molina Jiménez, Francisca Ugalde Triviño, Lola Arias González, Laura Relaño Rupérez, Carlos Casabona, Sergio Pérez Fernández, María Teresa Martín Domínguez, Verónica Fernández Pacheco, Jennifer Laserna Mendieta, Emilio José Muñoz Hernández, Patricia Arias Arias, Ángel Cano, Ainara Muñoz, Javier Lucendo, Alfredo J. Santander, Cecilio Majano, Pedro Majano Rodríguez, Pedro Lorenzo
author_role	author
author2	Ugalde Triviño, Lola Arias González, Laura Relaño Rupérez, Carlos Casabona, Sergio Pérez Fernández, María Teresa Martín Domínguez, Verónica Fernández Pacheco, Jennifer Laserna Mendieta, Emilio José Muñoz Hernández, Patricia Arias Arias, Ángel Cano, Ainara Muñoz, Javier Lucendo, Alfredo J. Santander, Cecilio Majano, Pedro Majano Rodríguez, Pedro Lorenzo
author2_role	author author author author author author author author author author author author author author author author
dc.contributor.none.fl_str_mv	Lucendo, Alfredo J. Santander, Cecilio Majano Rodríguez, Pedro Lorenzo Universidad Complutense de Madrid
dc.subject.none.fl_str_mv	576 616-092 Basic mechanisms Eosinophilic esophagitis Epithelium Proteomic Biología celular (Biología) Gastroenterología y hepatología 2407 Biología Celular 3205.03 Gastroenterología 3207 Patología
topic	576 616-092 Basic mechanisms Eosinophilic esophagitis Epithelium Proteomic Biología celular (Biología) Gastroenterología y hepatología 2407 Biología Celular 3205.03 Gastroenterología 3207 Patología
description	Background Eosinophilic esophagitis (EoE) is a chronic non-IgE-mediated allergic disease of the esophagus. An unbiased proteomics approach was performed to investigate pathophysiological changes in esophageal epithelium. Additionally, an RNAseq-based transcriptomic analysis in paired samples was also carried out. Methods Total proteins were purified from esophageal endoscopic biopsies in a cohort of adult EoE patients (n = 25) and healthy esophagus controls (n = 10). Differentially accumulated (DA) proteins in EoE patients compared to control tissues were characterized to identify altered biological processes and signaling pathways. Results were also compared with a quantitative proteome dataset of the human esophageal mucosa. Next, results were contrasted with those obtained after RNAseq analysis in paired samples. Finally, we matched up protein expression with two EoE-specific mRNA panels (EDP and Eso-EoE panel). Results A total of 1667 proteins were identified, of which 363 were DA in EoE. RNA sequencing in paired samples identified 1993 differentially expressed (DE) genes. Total RNA and protein levels positively correlated, especially in DE mRNA-proteins pairs. Pathway analysis of these proteins in EoE showed alterations in immune and inflammatory responses for the upregulated proteins, and in epithelial differentiation, cornification and keratinization in those downregulated. Interestingly, a set of DA proteins, including eosinophil-related and secreted proteins, were not detected at the mRNA level. Protein expression positively correlated with EDP and Eso-EoE, and corresponded with the most abundant proteins of the human esophageal proteome. Conclusions We unraveled for the first time key proteomic features involved in EoE pathogenesis. An integrative analysis of transcriptomic and proteomic datasets provides a deeper insight than transcriptomic alone into understanding complex disease mechanisms.
publishDate	2023
dc.date.none.fl_str_mv	2023 2023-10-01 2023 2023-10-01
dc.type.none.fl_str_mv	journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://hdl.handle.net/20.500.14352/102911
url	https://hdl.handle.net/20.500.14352/102911
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Wiley
publisher.none.fl_str_mv	Wiley
dc.source.none.fl_str_mv	reponame:Docta Complutense instname:Universidad Complutense de Madrid (UCM)
instname_str	Universidad Complutense de Madrid (UCM)
reponame_str	Docta Complutense
collection	Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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Proteomic analysis of the esophageal epithelium reveals key features of eosinophilic esophagitis pathophysiology

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