Competitive ELISA for N-Terminal pro-Brain Natriuretic Peptide (NT-proBNP) determination in human plasma

Brain natriuretic peptides (BNPs) are well established cardiovascular disease (CVD) biomarkers, which are released from the heart after ventricular wall stress. Particularly, the N-terminal proBNP (NT-proBNP) is a 76 aa long peptide which has been recognized as indicator for the diagnosis of heart f...

Descripción completa

Detalles Bibliográficos
Autores: Colom, Gloria, Salvador, Juan Pablo, Acosta, Gerardo, Royo, Miriam, Albericio, Fernando, Marco, María Pilar
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/217198
Acceso en línea:http://hdl.handle.net/10261/217198
Access Level:acceso abierto
Palabra clave:Brain natriuretic peptides
BNPs
Cardiovascular disease
CVD
id ES_f9eaa80e4db1a85c5ea74afdf8deb695
oai_identifier_str oai:digital.csic.es:10261/217198
network_acronym_str ES
network_name_str España
repository_id_str
spelling Competitive ELISA for N-Terminal pro-Brain Natriuretic Peptide (NT-proBNP) determination in human plasmaColom, GloriaSalvador, Juan PabloAcosta, GerardoRoyo, MiriamAlbericio, FernandoMarco, María PilarBrain natriuretic peptidesBNPsCardiovascular diseaseCVDBrain natriuretic peptides (BNPs) are well established cardiovascular disease (CVD) biomarkers, which are released from the heart after ventricular wall stress. Particularly, the N-terminal proBNP (NT-proBNP) is a 76 aa long peptide which has been recognized as indicator for the diagnosis of heart failure (HF) and is being used in routine tests in emergency rooms, when levels are above 0.4 ng mL-1. Here we report a new competitive ELISA for NT-proBNP which is able to detect this biomarker directly in undiluted human plasma samples. The ELISA has been the result of a rational design of an immunizing peptide hapten and the investigation of different immunochemical conditions, including heterologous competitors and distinct physico-chemical conditions. The ELISA developed is able to detect NT-proBNP with a LOD of 0.40±0.15 ng mL-1 in human plasma samples and to quantify this biomarker in the range comprised between 0.97±0.38 and 23.10±9.46 ng mL-1 with a good accuracy. The ELISA can measure simultaneously many samples in 1:30 h and it has been found robust and reproducible and being a great promise for their use on the diagnosis of the heart failure.This work has been funded by CAJAL4EU project (ICT-Joint Undertaking Action ENIAC-120215; MICINN FCCI-PLE2009-0168 and MICINN EUI-ENIAC-2011-4331). The Nb4D group is a consolidated research group (Grup de Recerca) of the Generalitat de Catalunya and has support from the Departament d’Universitats, Recerca i Societat de la Informació de la Generalitat de Catalunya (expedient: 2017 SGR 1441). CIBER-BBN is an initiative funded by the Spanish National Plan for Scientific and Technical Research and Innovation 2013-2016, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund. The ICTS “NANOBIOSIS”, and particularly the Custom Antibody Service (CAbS, IQAC-CSIC, CIBER-BBN), is acknowledged for the assistance and support related to the immunoreagents used in this work. The MS4N group thanks Spanish Ministerio de Economía, Industria y Competitividad SAF2014-60138-R), CIBER-BBN (CB06-01-0074), Generalitat de Catalunya (2017SGR1439). This work was partially performed at the facilities of NANBIOSIS (Units 2 and 3).Peer reviewedRoyal Society of Chemistry (UK)Ministerio de Economía y Competitividad (España)Salvador, Juan Pablo [0000-0002-3608-0634]Marco, María Pilar [0000-0002-2237-7197]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/217198reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2014-60138-Rhttps://doi.org/10.1039/D0AN00650ESíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2171982026-05-22T06:33:51Z
dc.title.none.fl_str_mv Competitive ELISA for N-Terminal pro-Brain Natriuretic Peptide (NT-proBNP) determination in human plasma
title Competitive ELISA for N-Terminal pro-Brain Natriuretic Peptide (NT-proBNP) determination in human plasma
spellingShingle Competitive ELISA for N-Terminal pro-Brain Natriuretic Peptide (NT-proBNP) determination in human plasma
Colom, Gloria
Brain natriuretic peptides
BNPs
Cardiovascular disease
CVD
title_short Competitive ELISA for N-Terminal pro-Brain Natriuretic Peptide (NT-proBNP) determination in human plasma
title_full Competitive ELISA for N-Terminal pro-Brain Natriuretic Peptide (NT-proBNP) determination in human plasma
title_fullStr Competitive ELISA for N-Terminal pro-Brain Natriuretic Peptide (NT-proBNP) determination in human plasma
title_full_unstemmed Competitive ELISA for N-Terminal pro-Brain Natriuretic Peptide (NT-proBNP) determination in human plasma
title_sort Competitive ELISA for N-Terminal pro-Brain Natriuretic Peptide (NT-proBNP) determination in human plasma
dc.creator.none.fl_str_mv Colom, Gloria
Salvador, Juan Pablo
Acosta, Gerardo
Royo, Miriam
Albericio, Fernando
Marco, María Pilar
author Colom, Gloria
author_facet Colom, Gloria
Salvador, Juan Pablo
Acosta, Gerardo
Royo, Miriam
Albericio, Fernando
Marco, María Pilar
author_role author
author2 Salvador, Juan Pablo
Acosta, Gerardo
Royo, Miriam
Albericio, Fernando
Marco, María Pilar
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Salvador, Juan Pablo [0000-0002-3608-0634]
Marco, María Pilar [0000-0002-2237-7197]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Brain natriuretic peptides
BNPs
Cardiovascular disease
CVD
topic Brain natriuretic peptides
BNPs
Cardiovascular disease
CVD
description Brain natriuretic peptides (BNPs) are well established cardiovascular disease (CVD) biomarkers, which are released from the heart after ventricular wall stress. Particularly, the N-terminal proBNP (NT-proBNP) is a 76 aa long peptide which has been recognized as indicator for the diagnosis of heart failure (HF) and is being used in routine tests in emergency rooms, when levels are above 0.4 ng mL-1. Here we report a new competitive ELISA for NT-proBNP which is able to detect this biomarker directly in undiluted human plasma samples. The ELISA has been the result of a rational design of an immunizing peptide hapten and the investigation of different immunochemical conditions, including heterologous competitors and distinct physico-chemical conditions. The ELISA developed is able to detect NT-proBNP with a LOD of 0.40±0.15 ng mL-1 in human plasma samples and to quantify this biomarker in the range comprised between 0.97±0.38 and 23.10±9.46 ng mL-1 with a good accuracy. The ELISA can measure simultaneously many samples in 1:30 h and it has been found robust and reproducible and being a great promise for their use on the diagnosis of the heart failure.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/217198
url http://hdl.handle.net/10261/217198
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2014-60138-R
https://doi.org/10.1039/D0AN00650E
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society of Chemistry (UK)
publisher.none.fl_str_mv Royal Society of Chemistry (UK)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425137374199808
score 15,811543

Similares en LA Referencia