Competitive ELISA for N-Terminal pro-Brain Natriuretic Peptide (NT-proBNP) determination in human plasma
Brain natriuretic peptides (BNPs) are well established cardiovascular disease (CVD) biomarkers, which are released from the heart after ventricular wall stress. Particularly, the N-terminal proBNP (NT-proBNP) is a 76 aa long peptide which has been recognized as indicator for the diagnosis of heart f...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/217198 |
| Acceso en línea: | http://hdl.handle.net/10261/217198 |
| Access Level: | acceso abierto |
| Palabra clave: | Brain natriuretic peptides BNPs Cardiovascular disease CVD |
| Sumario: | Brain natriuretic peptides (BNPs) are well established cardiovascular disease (CVD) biomarkers, which are released from the heart after ventricular wall stress. Particularly, the N-terminal proBNP (NT-proBNP) is a 76 aa long peptide which has been recognized as indicator for the diagnosis of heart failure (HF) and is being used in routine tests in emergency rooms, when levels are above 0.4 ng mL-1. Here we report a new competitive ELISA for NT-proBNP which is able to detect this biomarker directly in undiluted human plasma samples. The ELISA has been the result of a rational design of an immunizing peptide hapten and the investigation of different immunochemical conditions, including heterologous competitors and distinct physico-chemical conditions. The ELISA developed is able to detect NT-proBNP with a LOD of 0.40±0.15 ng mL-1 in human plasma samples and to quantify this biomarker in the range comprised between 0.97±0.38 and 23.10±9.46 ng mL-1 with a good accuracy. The ELISA can measure simultaneously many samples in 1:30 h and it has been found robust and reproducible and being a great promise for their use on the diagnosis of the heart failure. |
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