Distribution of peptidyl-glycine α-amidating mono-oxygenase (PAM) enzymes in normal human lung and in lung epithelial tumors

C-terminal α-amidation is a post-translational modification necessary for the biological activity of many regulatory peptides produced in the respiratory tract. This modification is a two-step process catalyzed by two separate enzyme activities, both derived from the peptidyl-glycine α- amidating mo...

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Autores: Saldise, Laura, Martínez, Alfredo [0000-0003-4882-4044], Montuenga, Luis M. [0000-0002-8739-1387], Treston, Anthony, Springall, David R., Polak, Julia M., Vázquez, Jesús J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1996
País:España
Institución:Universidad de La Rioja (UR)
Repositorio:RIUR. Repositorio Institucional de la Universidad de La Rioja
OAI Identifier:oai:portal.dialnet.es:doc/5d31a3a02999521056385011
Acceso en línea:https://investigacion.unirioja.es/documentos/5d31a3a02999521056385011
Access Level:acceso abierto
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spelling Distribution of peptidyl-glycine α-amidating mono-oxygenase (PAM) enzymes in normal human lung and in lung epithelial tumorsSaldise, LauraMartínez, Alfredo [0000-0003-4882-4044]Montuenga, Luis M. [0000-0002-8739-1387]Treston, AnthonySpringall, David R.Polak, Julia M.Vázquez, Jesús J.C-terminal α-amidation is a post-translational modification necessary for the biological activity of many regulatory peptides produced in the respiratory tract. This modification is a two-step process catalyzed by two separate enzyme activities, both derived from the peptidyl-glycine α- amidating mono-oxygenase (PAM) precursor. The distribution of these two enzymes, peptidyl-glycine α-hydroxylating monooxygenase (PHM) and peptidyl- α-hydroxyglycine α-amidating lyase (PAL), was studied in the normal lung and in lung tumors using immunocytochemical methods and in situ hybridization. In normal lung the enzymes were located in some cells of the airway epithelium and glands, the endothelium of blood vessels, some chondrocytes of the bronchial cartilage, the alveolar macrophages, smooth muscle cells, neurons of the intrinsic ganglia, and in myelinated nerves. A total of 24 lung tumors of seven different histological types were studied. All cases contained PAM-immunoreactive cells with various patterns of distribution. All immunoreactive cells were positive for the PHM antiserum but only some of them for the PAL antiserum. The distribution of PAM co- localizes with some other previously described amidated peptides, suggesting that amidation is an important physiological process taking place in the normal and malignant human lung tissue.Histochemical Society Inc.1996info:eu-repo/semantics/articleSubtype: Articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://investigacion.unirioja.es/documentos/5d31a3a02999521056385011reponame:RIUR. Repositorio Institucional de la Universidad de La Riojainstname:Universidad de La Rioja (UR)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1177/44.1.8543779info:eu-repo/semantics/altIdentifier/pmid/8543779info:eu-repo/semantics/altIdentifier/pissn/0022-1554Distribution of peptidyl-glycine α-amidating mono-oxygenase (PAM) enzymes in normal human lung and in lung epithelial tumors, 1996, vol. 44, núm. 1, pág. 3-12info:eu-repo/semantics/openAccessoai:portal.dialnet.es:doc/5d31a3a029995210563850112026-06-14T12:47:17Z
dc.title.none.fl_str_mv Distribution of peptidyl-glycine α-amidating mono-oxygenase (PAM) enzymes in normal human lung and in lung epithelial tumors
title Distribution of peptidyl-glycine α-amidating mono-oxygenase (PAM) enzymes in normal human lung and in lung epithelial tumors
spellingShingle Distribution of peptidyl-glycine α-amidating mono-oxygenase (PAM) enzymes in normal human lung and in lung epithelial tumors
Saldise, Laura
title_short Distribution of peptidyl-glycine α-amidating mono-oxygenase (PAM) enzymes in normal human lung and in lung epithelial tumors
title_full Distribution of peptidyl-glycine α-amidating mono-oxygenase (PAM) enzymes in normal human lung and in lung epithelial tumors
title_fullStr Distribution of peptidyl-glycine α-amidating mono-oxygenase (PAM) enzymes in normal human lung and in lung epithelial tumors
title_full_unstemmed Distribution of peptidyl-glycine α-amidating mono-oxygenase (PAM) enzymes in normal human lung and in lung epithelial tumors
title_sort Distribution of peptidyl-glycine α-amidating mono-oxygenase (PAM) enzymes in normal human lung and in lung epithelial tumors
dc.creator.none.fl_str_mv Saldise, Laura
Martínez, Alfredo [0000-0003-4882-4044]
Montuenga, Luis M. [0000-0002-8739-1387]
Treston, Anthony
Springall, David R.
Polak, Julia M.
Vázquez, Jesús J.
author Saldise, Laura
author_facet Saldise, Laura
Martínez, Alfredo [0000-0003-4882-4044]
Montuenga, Luis M. [0000-0002-8739-1387]
Treston, Anthony
Springall, David R.
Polak, Julia M.
Vázquez, Jesús J.
author_role author
author2 Martínez, Alfredo [0000-0003-4882-4044]
Montuenga, Luis M. [0000-0002-8739-1387]
Treston, Anthony
Springall, David R.
Polak, Julia M.
Vázquez, Jesús J.
author2_role author
author
author
author
author
author
description C-terminal α-amidation is a post-translational modification necessary for the biological activity of many regulatory peptides produced in the respiratory tract. This modification is a two-step process catalyzed by two separate enzyme activities, both derived from the peptidyl-glycine α- amidating mono-oxygenase (PAM) precursor. The distribution of these two enzymes, peptidyl-glycine α-hydroxylating monooxygenase (PHM) and peptidyl- α-hydroxyglycine α-amidating lyase (PAL), was studied in the normal lung and in lung tumors using immunocytochemical methods and in situ hybridization. In normal lung the enzymes were located in some cells of the airway epithelium and glands, the endothelium of blood vessels, some chondrocytes of the bronchial cartilage, the alveolar macrophages, smooth muscle cells, neurons of the intrinsic ganglia, and in myelinated nerves. A total of 24 lung tumors of seven different histological types were studied. All cases contained PAM-immunoreactive cells with various patterns of distribution. All immunoreactive cells were positive for the PHM antiserum but only some of them for the PAL antiserum. The distribution of PAM co- localizes with some other previously described amidated peptides, suggesting that amidation is an important physiological process taking place in the normal and malignant human lung tissue.
publishDate 1996
dc.date.none.fl_str_mv 1996
dc.type.none.fl_str_mv info:eu-repo/semantics/article
Subtype: Article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://investigacion.unirioja.es/documentos/5d31a3a02999521056385011
url https://investigacion.unirioja.es/documentos/5d31a3a02999521056385011
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1177/44.1.8543779
info:eu-repo/semantics/altIdentifier/pmid/8543779
info:eu-repo/semantics/altIdentifier/pissn/0022-1554
Distribution of peptidyl-glycine α-amidating mono-oxygenase (PAM) enzymes in normal human lung and in lung epithelial tumors, 1996, vol. 44, núm. 1, pág. 3-12
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Histochemical Society Inc.
publisher.none.fl_str_mv Histochemical Society Inc.
dc.source.none.fl_str_mv reponame:RIUR. Repositorio Institucional de la Universidad de La Rioja
instname:Universidad de La Rioja (UR)
instname_str Universidad de La Rioja (UR)
reponame_str RIUR. Repositorio Institucional de la Universidad de La Rioja
collection RIUR. Repositorio Institucional de la Universidad de La Rioja
repository.name.fl_str_mv
repository.mail.fl_str_mv
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