Localization of amidating enzymes (PAM) in rat gastrointestinal tract

We studied the distribution of the two enzymes involved in post-translational C-terminal alpha-amidation of regulatory peptides in rat digestive tract, using immunocytochemical methods and in situ hybridization techniques. The enzymes were located in most of the fibers and neurons of the myenteric a...

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Detalles Bibliográficos
Autores: Martinez, A. (Alfredo)|||/items/ce27c09e-454d-4645-986e-713760a3730d, Burrell, M.A. (María Ángela)|||/items/27fe7d6b-4d2b-4917-91af-68de4749f75b, Kuijk, M. (Marjolein)|||/items/975bacec-5070-4d15-bb4e-782d3beab9e3, Montuenga-Badia, L.M. (Luis M.)|||/items/4c999705-b2c9-45ac-ba13-3f18594ae596, Treston, A.M. (Anthony M.)|||/items/2a2177c8-e6d5-486c-841e-62a5423a037b, Cuttitta, F. (Frank)|||/items/0f0da08c-0efe-4d3c-97ca-44c38a26e188, Polak, J.M. (Julia M.)|||/items/27bc2706-e444-4ece-99d6-b2bde0c966b4
Tipo de recurso: artículo
Fecha de publicación:1993
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/20131
Acceso en línea:https://hdl.handle.net/10171/20131
Access Level:acceso abierto
Palabra clave:Amidation
PAM
PHM
PAL
Rat gastrointestinal tract
GLP1
Immunocytochemistry
In situ hybridization
Descripción
Sumario:We studied the distribution of the two enzymes involved in post-translational C-terminal alpha-amidation of regulatory peptides in rat digestive tract, using immunocytochemical methods and in situ hybridization techniques. The enzymes were located in most of the fibers and neurons of the myenteric and submucous plexus throughout the entire digestive tract and in endocrine cells of the stomach and colon. Staining of reverse-face serial sections demonstrated that the enzymes in endocrine cells of the stomach co-localized with gastrin in the bottom of the gastric glands. Some gastrin-immunoreactive cells near the neck of the gland were negative for PAM, suggesting that amidation takes place only in the more mature cells. In the colon all cells immunoreactive for glucagon and GLP1 were also positive for peptidylglycine alpha-hydroxylating monooxygenase (PHM) but not for peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). The absence of immunoreactivity for the amidating enzymes in endocrine cells of the small intestine, known to produce C-terminally amidated peptides, suggests the existence of other amidating enzymes.

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