Understanding the effect of the nature of the nucleobase in the loops on the stability of the i-motif structure

The nature and the length of loops connecting cytosine tracts in i-motif structures may affect their stability. In this work, the influence of the nature of the nucleobases located in two of the loops of an intramolecular i-motif is studied using spectroscopy, separation techniques, and multivariate...

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Autores: Benabou, Sanae, Garavís, Miguel, Lyonnais, Sébastien, Eritja Casadellà, Ramón, Bustamante González, Carlos Alberto, Gargallo, Raimundo
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2016
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/131028
Acceso en línea:http://hdl.handle.net/10261/131028
Access Level:acceso abierto
Palabra clave:Nucleobases
Cytosine tracts
Spectroscopy
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spelling Understanding the effect of the nature of the nucleobase in the loops on the stability of the i-motif structureBenabou, SanaeGaravís, MiguelLyonnais, SébastienEritja Casadellà, RamónBustamante González, Carlos AlbertoGargallo, RaimundoNucleobasesCytosine tractsSpectroscopyThe nature and the length of loops connecting cytosine tracts in i-motif structures may affect their stability. In this work, the influence of the nature of the nucleobases located in two of the loops of an intramolecular i-motif is studied using spectroscopy, separation techniques, and multivariate data analysis. The insertion of bases other than thymine induces an additional acid-base equilibrium with pKa ∼ 4.5. The presence of two guanine bases in the loops, placed opposite to each other, decreases the thermal stability of the structure. In contrast, thymine and cytosine bases in these positions stabilize the structure. © the Owner Societies 2016.Anna Sadurnı´ (University of Barcelona) is acknowledged for carrying out some experiments. We acknowledge funding from the Spanish government (BFU2014-52864-R, CTQ2012-38616- C02-02 and CTQ2014-52588-R). This research has been recognized by the regional Catalan authorities (2014 SGR 1106).Peer reviewedRoyal Society of Chemistry (UK)Ministerio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201620162016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/131028reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2014-52588-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2014-52864-Rhttp://dx.doi.org/10.1039/c5cp07428bSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1310282026-05-22T06:33:51Z
dc.title.none.fl_str_mv Understanding the effect of the nature of the nucleobase in the loops on the stability of the i-motif structure
title Understanding the effect of the nature of the nucleobase in the loops on the stability of the i-motif structure
spellingShingle Understanding the effect of the nature of the nucleobase in the loops on the stability of the i-motif structure
Benabou, Sanae
Nucleobases
Cytosine tracts
Spectroscopy
title_short Understanding the effect of the nature of the nucleobase in the loops on the stability of the i-motif structure
title_full Understanding the effect of the nature of the nucleobase in the loops on the stability of the i-motif structure
title_fullStr Understanding the effect of the nature of the nucleobase in the loops on the stability of the i-motif structure
title_full_unstemmed Understanding the effect of the nature of the nucleobase in the loops on the stability of the i-motif structure
title_sort Understanding the effect of the nature of the nucleobase in the loops on the stability of the i-motif structure
dc.creator.none.fl_str_mv Benabou, Sanae
Garavís, Miguel
Lyonnais, Sébastien
Eritja Casadellà, Ramón
Bustamante González, Carlos Alberto
Gargallo, Raimundo
author Benabou, Sanae
author_facet Benabou, Sanae
Garavís, Miguel
Lyonnais, Sébastien
Eritja Casadellà, Ramón
Bustamante González, Carlos Alberto
Gargallo, Raimundo
author_role author
author2 Garavís, Miguel
Lyonnais, Sébastien
Eritja Casadellà, Ramón
Bustamante González, Carlos Alberto
Gargallo, Raimundo
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Nucleobases
Cytosine tracts
Spectroscopy
topic Nucleobases
Cytosine tracts
Spectroscopy
description The nature and the length of loops connecting cytosine tracts in i-motif structures may affect their stability. In this work, the influence of the nature of the nucleobases located in two of the loops of an intramolecular i-motif is studied using spectroscopy, separation techniques, and multivariate data analysis. The insertion of bases other than thymine induces an additional acid-base equilibrium with pKa ∼ 4.5. The presence of two guanine bases in the loops, placed opposite to each other, decreases the thermal stability of the structure. In contrast, thymine and cytosine bases in these positions stabilize the structure. © the Owner Societies 2016.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/131028
url http://hdl.handle.net/10261/131028
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2014-52588-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2014-52864-R
http://dx.doi.org/10.1039/c5cp07428b
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society of Chemistry (UK)
publisher.none.fl_str_mv Royal Society of Chemistry (UK)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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