New Insights on Arabidopsis thaliana Root Adaption to Ammonium Nutrition by the Use of a Quantitative Proteomic Approach

Nitrogen is an essential element for plant nutrition. Nitrate and ammonium are the two major inorganic nitrogen forms available for plant growth. Plant preference for one or the other form depends on the interplay between plant genetic background and environmental variables. Ammonium-based fertiliza...

Descripción completa

Detalles Bibliográficos
Autores: Coleto Reyes, Inmaculada, Vega Mas, Izargi Aida, Glauser, Gaetan, González Moro, María Begoña, Marino Bilbao, Daniel, Ariz, Idoia
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/32166
Acceso en línea:http://hdl.handle.net/10810/32166
Access Level:acceso abierto
Palabra clave:arabidopsis thaliana
ammonium
carbon metabolism
nitrate
nitrogen metabolism
proteomics
root
secondary metabolism
id ES_f93ebc66f82b37eadaa0c5e08d79bc3c
oai_identifier_str oai:addi.ehu.eus:10810/32166
network_acronym_str ES
network_name_str España
repository_id_str
spelling New Insights on Arabidopsis thaliana Root Adaption to Ammonium Nutrition by the Use of a Quantitative Proteomic ApproachColeto Reyes, InmaculadaVega Mas, Izargi AidaGlauser, GaetanGonzález Moro, María BegoñaMarino Bilbao, DanielAriz, Idoiaarabidopsis thalianaammoniumcarbon metabolismnitratenitrogen metabolismproteomicsrootsecondary metabolismNitrogen is an essential element for plant nutrition. Nitrate and ammonium are the two major inorganic nitrogen forms available for plant growth. Plant preference for one or the other form depends on the interplay between plant genetic background and environmental variables. Ammonium-based fertilization has been shown less environmentally harmful compared to nitrate fertilization, because of reducing, among others, nitrate leaching and nitrous oxide emissions. However, ammonium nutrition may become a stressful situation for a wide range of plant species when the ion is present at high concentrations. Although studied for long time, there is still an important lack of knowledge to explain plant tolerance or sensitivity towards ammonium nutrition. In this context, we performed a comparative proteomic study in roots of Arabidopsis thaliana plants grown under exclusive ammonium or nitrate supply. We identified and quantified 68 proteins with differential abundance between both conditions. These proteins revealed new potential important players on root response to ammonium nutrition, such as H⁺-consuming metabolic pathways to regulate pH homeostasis and specific secondary metabolic pathways like brassinosteroid and glucosinolate biosynthetic pathways.This research was financially supported by the Basque Government (IT932-16), the Spanish Ministry of Economy and Competitiveness (BIO2014-56271-R and BIO2017-84035-R co-funded by FEDER). I.A. was supported by a Juan de la Cierva postdoctoral contract (IJCI-2015-26002) from the Spanish Ministry of Economy and Competitiveness. I.C. was supported by a postdoctoral fellowship from the University of the Basque Country UPV/EHU.MDPI201920192019info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/32166reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MINECO/IJCI-2015-26002/info:eu-repo/grantAgreement/MINECO/BIO2014-56271-R/info:eu-repo/grantAgreement/MINECO/BIO2017-84035-R/https://www.mdpi.com/1422-0067/20/4/814info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).Atribución 3.0 Españaoai:addi.ehu.eus:10810/321662026-06-18T09:23:17Z
dc.title.none.fl_str_mv New Insights on Arabidopsis thaliana Root Adaption to Ammonium Nutrition by the Use of a Quantitative Proteomic Approach
title New Insights on Arabidopsis thaliana Root Adaption to Ammonium Nutrition by the Use of a Quantitative Proteomic Approach
spellingShingle New Insights on Arabidopsis thaliana Root Adaption to Ammonium Nutrition by the Use of a Quantitative Proteomic Approach
Coleto Reyes, Inmaculada
arabidopsis thaliana
ammonium
carbon metabolism
nitrate
nitrogen metabolism
proteomics
root
secondary metabolism
title_short New Insights on Arabidopsis thaliana Root Adaption to Ammonium Nutrition by the Use of a Quantitative Proteomic Approach
title_full New Insights on Arabidopsis thaliana Root Adaption to Ammonium Nutrition by the Use of a Quantitative Proteomic Approach
title_fullStr New Insights on Arabidopsis thaliana Root Adaption to Ammonium Nutrition by the Use of a Quantitative Proteomic Approach
title_full_unstemmed New Insights on Arabidopsis thaliana Root Adaption to Ammonium Nutrition by the Use of a Quantitative Proteomic Approach
title_sort New Insights on Arabidopsis thaliana Root Adaption to Ammonium Nutrition by the Use of a Quantitative Proteomic Approach
dc.creator.none.fl_str_mv Coleto Reyes, Inmaculada
Vega Mas, Izargi Aida
Glauser, Gaetan
González Moro, María Begoña
Marino Bilbao, Daniel
Ariz, Idoia
author Coleto Reyes, Inmaculada
author_facet Coleto Reyes, Inmaculada
Vega Mas, Izargi Aida
Glauser, Gaetan
González Moro, María Begoña
Marino Bilbao, Daniel
Ariz, Idoia
author_role author
author2 Vega Mas, Izargi Aida
Glauser, Gaetan
González Moro, María Begoña
Marino Bilbao, Daniel
Ariz, Idoia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv arabidopsis thaliana
ammonium
carbon metabolism
nitrate
nitrogen metabolism
proteomics
root
secondary metabolism
topic arabidopsis thaliana
ammonium
carbon metabolism
nitrate
nitrogen metabolism
proteomics
root
secondary metabolism
description Nitrogen is an essential element for plant nutrition. Nitrate and ammonium are the two major inorganic nitrogen forms available for plant growth. Plant preference for one or the other form depends on the interplay between plant genetic background and environmental variables. Ammonium-based fertilization has been shown less environmentally harmful compared to nitrate fertilization, because of reducing, among others, nitrate leaching and nitrous oxide emissions. However, ammonium nutrition may become a stressful situation for a wide range of plant species when the ion is present at high concentrations. Although studied for long time, there is still an important lack of knowledge to explain plant tolerance or sensitivity towards ammonium nutrition. In this context, we performed a comparative proteomic study in roots of Arabidopsis thaliana plants grown under exclusive ammonium or nitrate supply. We identified and quantified 68 proteins with differential abundance between both conditions. These proteins revealed new potential important players on root response to ammonium nutrition, such as H⁺-consuming metabolic pathways to regulate pH homeostasis and specific secondary metabolic pathways like brassinosteroid and glucosinolate biosynthetic pathways.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/32166
url http://hdl.handle.net/10810/32166
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/IJCI-2015-26002/
info:eu-repo/grantAgreement/MINECO/BIO2014-56271-R/
info:eu-repo/grantAgreement/MINECO/BIO2017-84035-R/
https://www.mdpi.com/1422-0067/20/4/814
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869425076909113344
score 15,301603

Similares en LA Referencia