Regulation of peroxidase transcript levels in liquid cultures of the ligninolytic fungus Pleurotus eryngii

A versatile peroxidase able to oxidize Mn(2+) as well as phenolic and nonphenolic aromatic compounds is produced in peptone-containing liquid cultures of Pleurotus eryngii encoded by the gene mnpl. The regulation of its transcript levels was investigated by Northern blotting of total RNA. High-perox...

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Autores: Ruiz-Dueñas, F. J., Guillén, Francisco, Camarero, Susana, Pérez-Boada, Marta, Martínez, María Jesús, Martínez, Ángel T.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1999
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::929f9bc11bea40d71b4d3d8cc2796f2c
Acceso en línea:http://hdl.handle.net/10261/429127
https://api.elsevier.com/content/abstract/scopus_id/0032834008
Access Level:acceso abierto
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spelling Regulation of peroxidase transcript levels in liquid cultures of the ligninolytic fungus Pleurotus eryngiiRuiz-Dueñas, F. J.Guillén, FranciscoCamarero, SusanaPérez-Boada, MartaMartínez, María JesúsMartínez, Ángel T.A versatile peroxidase able to oxidize Mn(2+) as well as phenolic and nonphenolic aromatic compounds is produced in peptone-containing liquid cultures of Pleurotus eryngii encoded by the gene mnpl. The regulation of its transcript levels was investigated by Northern blotting of total RNA. High-peroxidase transcripts and activity were found in cultures grown in glucose-peptone medium, whereas only basal levels were detected in glucose-ammonium medium. The addition of more than 25 microM Mn(2+) to the former medium did not result in detectable peroxidase transcripts or activity. Potential regulators were also added to isolated mycelium. In this way, it was shown that high transcript levels (in peroxidase-expressing mycelium) were maintained on peptone, whereas expression was not induced in short-term incubation experiments. Similar results were obtained with Mn(2+) ions. Strong induction of mnpl expression was caused by exogenous H(2)O(2) or by continuous H(2)O(2) generation during redox cycling of menadione. By the use of the latter system in the presence of Fe(3+), which catalyzes the reduction of H(2)O(2) to hydroxyl radical, it was shown for the first time that the presence of this strong oxidant causes a rapid increase of the transcripts of a ligninolytic peroxidase. In conclusion, peptone and Mn(2+) affect the levels of transcripts of this versatile peroxidase in culture, and reduced oxygen species induce short-term expression in isolated mycelium, probably via a stress response mechanism.We thank E. Varela and L. Botella for contributions to obtain themnpl2 probe, A. Dı́az for DNA sequencing, and A. Guijarro and T. Raposo for skillful technical assistance. This work was partially supported by the EU contract AIR2-CT93-1219 (Biological delignification in paper manufacture) and by Spanish biotechnology project BIO96-393 (Evaluation of enzymatic and radical-mediated mechanisms in lignin degradation by fungi from the generaPleurotus and Phanerochaete).Peer reviewedAmerican Society for MicrobiologyEuropean CommissionCamarero, Susana [0000-0002-2812-895X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620261999info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/429127https://api.elsevier.com/content/abstract/scopus_id/0032834008reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#AIR2-CT93-1219BIO96-393https://doi.org/10.1128/AEM.65.10.4458-4463.1999Síinfo:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::929f9bc11bea40d71b4d3d8cc2796f2c2026-05-22T06:33:51Z
dc.title.none.fl_str_mv Regulation of peroxidase transcript levels in liquid cultures of the ligninolytic fungus Pleurotus eryngii
title Regulation of peroxidase transcript levels in liquid cultures of the ligninolytic fungus Pleurotus eryngii
spellingShingle Regulation of peroxidase transcript levels in liquid cultures of the ligninolytic fungus Pleurotus eryngii
Ruiz-Dueñas, F. J.
title_short Regulation of peroxidase transcript levels in liquid cultures of the ligninolytic fungus Pleurotus eryngii
title_full Regulation of peroxidase transcript levels in liquid cultures of the ligninolytic fungus Pleurotus eryngii
title_fullStr Regulation of peroxidase transcript levels in liquid cultures of the ligninolytic fungus Pleurotus eryngii
title_full_unstemmed Regulation of peroxidase transcript levels in liquid cultures of the ligninolytic fungus Pleurotus eryngii
title_sort Regulation of peroxidase transcript levels in liquid cultures of the ligninolytic fungus Pleurotus eryngii
dc.creator.none.fl_str_mv Ruiz-Dueñas, F. J.
Guillén, Francisco
Camarero, Susana
Pérez-Boada, Marta
Martínez, María Jesús
Martínez, Ángel T.
author Ruiz-Dueñas, F. J.
author_facet Ruiz-Dueñas, F. J.
Guillén, Francisco
Camarero, Susana
Pérez-Boada, Marta
Martínez, María Jesús
Martínez, Ángel T.
author_role author
author2 Guillén, Francisco
Camarero, Susana
Pérez-Boada, Marta
Martínez, María Jesús
Martínez, Ángel T.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Camarero, Susana [0000-0002-2812-895X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description A versatile peroxidase able to oxidize Mn(2+) as well as phenolic and nonphenolic aromatic compounds is produced in peptone-containing liquid cultures of Pleurotus eryngii encoded by the gene mnpl. The regulation of its transcript levels was investigated by Northern blotting of total RNA. High-peroxidase transcripts and activity were found in cultures grown in glucose-peptone medium, whereas only basal levels were detected in glucose-ammonium medium. The addition of more than 25 microM Mn(2+) to the former medium did not result in detectable peroxidase transcripts or activity. Potential regulators were also added to isolated mycelium. In this way, it was shown that high transcript levels (in peroxidase-expressing mycelium) were maintained on peptone, whereas expression was not induced in short-term incubation experiments. Similar results were obtained with Mn(2+) ions. Strong induction of mnpl expression was caused by exogenous H(2)O(2) or by continuous H(2)O(2) generation during redox cycling of menadione. By the use of the latter system in the presence of Fe(3+), which catalyzes the reduction of H(2)O(2) to hydroxyl radical, it was shown for the first time that the presence of this strong oxidant causes a rapid increase of the transcripts of a ligninolytic peroxidase. In conclusion, peptone and Mn(2+) affect the levels of transcripts of this versatile peroxidase in culture, and reduced oxygen species induce short-term expression in isolated mycelium, probably via a stress response mechanism.
publishDate 1999
dc.date.none.fl_str_mv 1999
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/429127
https://api.elsevier.com/content/abstract/scopus_id/0032834008
url http://hdl.handle.net/10261/429127
https://api.elsevier.com/content/abstract/scopus_id/0032834008
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
AIR2-CT93-1219
BIO96-393
https://doi.org/10.1128/AEM.65.10.4458-4463.1999
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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repository.mail.fl_str_mv
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