Abf1 and other general regulatory factors control ribosome biogenesis gene expression in budding yeast

Ribosome biogenesis in Saccharomyces cerevisiae involves a regulon of >200 genes (Ribi genes) coordinately regulated in response to nutrient availability and cellular growth rate. Two cis-acting elements called PAC and RRPE are known to mediate Ribi gene repression in response to nutritional...

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Detalles Bibliográficos
Autores: Bosio, Maria Cristina, Fermi, Beatrice, Spagnoli, Gloria, Levati, Elisabetta, Rubbi, Liudmilla, Ferrari, Roberto, Pellegrini, Matteo, Dieci, Giorgio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/34991
Acceso en línea:http://hdl.handle.net/10230/34991
http://dx.doi.org/10.1093/nar/gkx058
Access Level:acceso abierto
Palabra clave:Dead-box rna helicases
Dna-binding proteins
Gene expression regulation
Glucose
Histone deacetylase 1
Saccharomyces cerevisiae
Ribosomes
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spelling Abf1 and other general regulatory factors control ribosome biogenesis gene expression in budding yeastBosio, Maria CristinaFermi, BeatriceSpagnoli, GloriaLevati, ElisabettaRubbi, LiudmillaFerrari, RobertoPellegrini, MatteoDieci, GiorgioDead-box rna helicasesDna-binding proteinsGene expression regulationGlucoseHistone deacetylase 1Saccharomyces cerevisiaeRibosomesRibosome biogenesis in Saccharomyces cerevisiae involves a regulon of >200 genes (Ribi genes) coordinately regulated in response to nutrient availability and cellular growth rate. Two cis-acting elements called PAC and RRPE are known to mediate Ribi gene repression in response to nutritional downshift. Here, we show that most Ribi gene promoters also contain binding sites for one or more General Regulatory Factors (GRFs), most frequently Abf1 and Reb1, and that these factors are enriched in vivo at Ribi promoters. Abf1/Reb1/Tbf1 promoter association was required for full Ribi gene expression in rich medium and for its modulation in response to glucose starvation, characterized by a rapid drop followed by slow recovery. Such a response did not entail changes in Abf1 occupancy, but it was paralleled by a quick increase, followed by slow decrease, in Rpd3L histone deacetylase occupancy. Remarkably, Abf1 site disruption also abolished Rpd3L complex recruitment in response to starvation. Extensive mutational analysis of the DBP7 promoter revealed a complex interplay of Tbf1 sites, PAC and RRPE in the transcriptional regulation of this Ribi gene. Our observations point to GRFs as new multifaceted players in Ribi gene regulation both during exponential growth and under repressive conditions.Oxford University Press201820182017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/34991http://dx.doi.org/10.1093/nar/gkx058reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésNucleic Acids Res. 2017 May 5;45(8):4493-4506© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.comhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/349912026-05-29T05:05:01Z
dc.title.none.fl_str_mv Abf1 and other general regulatory factors control ribosome biogenesis gene expression in budding yeast
title Abf1 and other general regulatory factors control ribosome biogenesis gene expression in budding yeast
spellingShingle Abf1 and other general regulatory factors control ribosome biogenesis gene expression in budding yeast
Bosio, Maria Cristina
Dead-box rna helicases
Dna-binding proteins
Gene expression regulation
Glucose
Histone deacetylase 1
Saccharomyces cerevisiae
Ribosomes
title_short Abf1 and other general regulatory factors control ribosome biogenesis gene expression in budding yeast
title_full Abf1 and other general regulatory factors control ribosome biogenesis gene expression in budding yeast
title_fullStr Abf1 and other general regulatory factors control ribosome biogenesis gene expression in budding yeast
title_full_unstemmed Abf1 and other general regulatory factors control ribosome biogenesis gene expression in budding yeast
title_sort Abf1 and other general regulatory factors control ribosome biogenesis gene expression in budding yeast
dc.creator.none.fl_str_mv Bosio, Maria Cristina
Fermi, Beatrice
Spagnoli, Gloria
Levati, Elisabetta
Rubbi, Liudmilla
Ferrari, Roberto
Pellegrini, Matteo
Dieci, Giorgio
author Bosio, Maria Cristina
author_facet Bosio, Maria Cristina
Fermi, Beatrice
Spagnoli, Gloria
Levati, Elisabetta
Rubbi, Liudmilla
Ferrari, Roberto
Pellegrini, Matteo
Dieci, Giorgio
author_role author
author2 Fermi, Beatrice
Spagnoli, Gloria
Levati, Elisabetta
Rubbi, Liudmilla
Ferrari, Roberto
Pellegrini, Matteo
Dieci, Giorgio
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Dead-box rna helicases
Dna-binding proteins
Gene expression regulation
Glucose
Histone deacetylase 1
Saccharomyces cerevisiae
Ribosomes
topic Dead-box rna helicases
Dna-binding proteins
Gene expression regulation
Glucose
Histone deacetylase 1
Saccharomyces cerevisiae
Ribosomes
description Ribosome biogenesis in Saccharomyces cerevisiae involves a regulon of >200 genes (Ribi genes) coordinately regulated in response to nutrient availability and cellular growth rate. Two cis-acting elements called PAC and RRPE are known to mediate Ribi gene repression in response to nutritional downshift. Here, we show that most Ribi gene promoters also contain binding sites for one or more General Regulatory Factors (GRFs), most frequently Abf1 and Reb1, and that these factors are enriched in vivo at Ribi promoters. Abf1/Reb1/Tbf1 promoter association was required for full Ribi gene expression in rich medium and for its modulation in response to glucose starvation, characterized by a rapid drop followed by slow recovery. Such a response did not entail changes in Abf1 occupancy, but it was paralleled by a quick increase, followed by slow decrease, in Rpd3L histone deacetylase occupancy. Remarkably, Abf1 site disruption also abolished Rpd3L complex recruitment in response to starvation. Extensive mutational analysis of the DBP7 promoter revealed a complex interplay of Tbf1 sites, PAC and RRPE in the transcriptional regulation of this Ribi gene. Our observations point to GRFs as new multifaceted players in Ribi gene regulation both during exponential growth and under repressive conditions.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/34991
http://dx.doi.org/10.1093/nar/gkx058
url http://hdl.handle.net/10230/34991
http://dx.doi.org/10.1093/nar/gkx058
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Nucleic Acids Res. 2017 May 5;45(8):4493-4506
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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