Variable-Temperature Size Exclusion Chromatography for the Study of the Structural Changes in G-Quadruplex

The conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature size exclusion chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture o...

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Authors: Benabou, Sanae, Gargallo, Raimundo, Eritja Casadellà, Ramón
Format: article
Status:Published version
Publication Date:2013
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/125376
Online Access:http://hdl.handle.net/10261/125376
Access Level:Open access
Keyword:G-quadruplex
Chromatography
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spelling Variable-Temperature Size Exclusion Chromatography for the Study of the Structural Changes in G-QuadruplexBenabou, SanaeGargallo, RaimundoEritja Casadellà, RamónG-quadruplexChromatographyThe conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature size exclusion chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture of monomeric and multimeric G-quadruplexes. Appropriate mutation of several bases in the wild sequence produces the shift from parallel to antiparallel G-quadruplex, as well as the disappearance of multimeric species. The shift from the antiparallel to the parallel conformation induced by temperature is reflected in both CD and SEC profiles.The authors acknowledge funding from the Spanish Government (CTQ2012-38616-C02-02 and CTQ2010-20541-C03-01).Peer reviewedHindawi Publishing CorporationConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201520152013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/125376reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1155/2013/631875Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1253762026-05-22T06:33:51Z
dc.title.none.fl_str_mv Variable-Temperature Size Exclusion Chromatography for the Study of the Structural Changes in G-Quadruplex
title Variable-Temperature Size Exclusion Chromatography for the Study of the Structural Changes in G-Quadruplex
spellingShingle Variable-Temperature Size Exclusion Chromatography for the Study of the Structural Changes in G-Quadruplex
Benabou, Sanae
G-quadruplex
Chromatography
title_short Variable-Temperature Size Exclusion Chromatography for the Study of the Structural Changes in G-Quadruplex
title_full Variable-Temperature Size Exclusion Chromatography for the Study of the Structural Changes in G-Quadruplex
title_fullStr Variable-Temperature Size Exclusion Chromatography for the Study of the Structural Changes in G-Quadruplex
title_full_unstemmed Variable-Temperature Size Exclusion Chromatography for the Study of the Structural Changes in G-Quadruplex
title_sort Variable-Temperature Size Exclusion Chromatography for the Study of the Structural Changes in G-Quadruplex
dc.creator.none.fl_str_mv Benabou, Sanae
Gargallo, Raimundo
Eritja Casadellà, Ramón
author Benabou, Sanae
author_facet Benabou, Sanae
Gargallo, Raimundo
Eritja Casadellà, Ramón
author_role author
author2 Gargallo, Raimundo
Eritja Casadellà, Ramón
author2_role author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv G-quadruplex
Chromatography
topic G-quadruplex
Chromatography
description The conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature size exclusion chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture of monomeric and multimeric G-quadruplexes. Appropriate mutation of several bases in the wild sequence produces the shift from parallel to antiparallel G-quadruplex, as well as the disappearance of multimeric species. The shift from the antiparallel to the parallel conformation induced by temperature is reflected in both CD and SEC profiles.
publishDate 2013
dc.date.none.fl_str_mv 2013
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/125376
url http://hdl.handle.net/10261/125376
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1155/2013/631875
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869424985581289472
score 15,812429

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