Variable-temperature size exclusion chromatography for the study of the structural changes in G-quadruplex
The conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature Size Exclusion Chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture o...
| Autores: | , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | España |
| Recursos: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/151262 |
| Acesso em linha: | https://hdl.handle.net/2445/151262 |
| Access Level: | acceso abierto |
| Palavra-chave: | Àcids nucleics G-estructures Oligonucleòtids Nucleic acids G-structures Oligonucleotides |
| Resumo: | The conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature Size Exclusion Chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture of monomeric and multimeric G-quadruplex. Appropriate mutation of several bases in the wild sequence produces the shift from parallel to antiparallel G-quadruplex, as well as the disappearance of multimeric species. The shift from the antiparallel to the parallel conformation induced by temperature is reflected in both CD and SEC profiles. |
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