Uncovering de novo gene birth in yeast using deep transcriptomics

De novo gene origination has been recently established as an important mechanism for the formation of new genes. In organisms with a large genome, intergenic and intronic regions provide plenty of raw material for new transcriptional events to occur, but little is know about how de novo transcripts...

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Authors: Blevins, William Robert, 1987-, Ruiz Orera, Jorge, 1988-, Messeguer, Xavier, Blasco Moreno, Bernat, 1986-, Villanueva Cañas, José Luis, 1984-, Espinar, Lorena, Díez Antón, Juana, 1962-, Carey, Lucas, 1980-, Albà Soler, Mar
Format: article
Status:Published version
Publication Date:2021
Country:España
Institution:Universitat Pompeu Fabra
Repository:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/46484
Online Access:http://hdl.handle.net/10230/46484
http://dx.doi.org/10.1038/s41467-021-20911-3
Access Level:Open access
Keyword:Evolvability
Fungal genetics
Molecular evolution
Transcriptomics
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spelling Uncovering de novo gene birth in yeast using deep transcriptomicsBlevins, William Robert, 1987-Ruiz Orera, Jorge, 1988-Messeguer, XavierBlasco Moreno, Bernat, 1986-Villanueva Cañas, José Luis, 1984-Espinar, LorenaDíez Antón, Juana, 1962-Carey, Lucas, 1980-Albà Soler, MarEvolvabilityFungal geneticsMolecular evolutionTranscriptomicsDe novo gene origination has been recently established as an important mechanism for the formation of new genes. In organisms with a large genome, intergenic and intronic regions provide plenty of raw material for new transcriptional events to occur, but little is know about how de novo transcripts originate in more densely-packed genomes. Here, we identify 213 de novo originated transcripts in Saccharomyces cerevisiae using deep transcriptomics and genomic synteny information from multiple yeast species grown in two different conditions. We find that about half of the de novo transcripts are expressed from regions which already harbor other genes in the opposite orientation; these transcripts show similar expression changes in response to stress as their overlapping counterparts, and some appear to translate small proteins. Thus, a large fraction of de novo genes in yeast are likely to co-evolve with already existing genes.The work was funded by grants PGC2018-094091-B-I00, BFU2015-65235-P, BFU2015-68351-P, BFU2016-80039-R, TIN2015-69175-C4-3-R and RTI2018-094403-B-C33 from Spanish Government—FEDER (EU), and from grant PT17/0009/0014 from Instituto de Salud Carlos III—FEDER. We also received funding from the “Maria de Maeztu” Programme for Units of Excellence in R&D (MDM-2014-0370) and from Agència de Gestió d’Ajuts Universitaris i de Recerca Generalitat de Catalunya (AGAUR), grants number 2014SGR1121, 2014SGR0974, 2017SGR1054 and 2017SGR01020 and, predoctoral fellowship (FI) to W.R.B.Nature Research202120212021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/46484http://dx.doi.org/10.1038/s41467-021-20911-3reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésNat Commun. 2021; 12(1):604info:eu-repo/grantAgreement/ES/2PE/PGC2018-094091-B-I00info:eu-repo/grantAgreement/ES/1PE/BFU2015-65235-Pinfo:eu-repo/grantAgreement/ES/1PE/BFU2015-68351-Pinfo:eu-repo/grantAgreement/ES/1PE/BFU2016-80039-R© The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/464842026-06-12T07:21:37Z
dc.title.none.fl_str_mv Uncovering de novo gene birth in yeast using deep transcriptomics
title Uncovering de novo gene birth in yeast using deep transcriptomics
spellingShingle Uncovering de novo gene birth in yeast using deep transcriptomics
Blevins, William Robert, 1987-
Evolvability
Fungal genetics
Molecular evolution
Transcriptomics
title_short Uncovering de novo gene birth in yeast using deep transcriptomics
title_full Uncovering de novo gene birth in yeast using deep transcriptomics
title_fullStr Uncovering de novo gene birth in yeast using deep transcriptomics
title_full_unstemmed Uncovering de novo gene birth in yeast using deep transcriptomics
title_sort Uncovering de novo gene birth in yeast using deep transcriptomics
dc.creator.none.fl_str_mv Blevins, William Robert, 1987-
Ruiz Orera, Jorge, 1988-
Messeguer, Xavier
Blasco Moreno, Bernat, 1986-
Villanueva Cañas, José Luis, 1984-
Espinar, Lorena
Díez Antón, Juana, 1962-
Carey, Lucas, 1980-
Albà Soler, Mar
author Blevins, William Robert, 1987-
author_facet Blevins, William Robert, 1987-
Ruiz Orera, Jorge, 1988-
Messeguer, Xavier
Blasco Moreno, Bernat, 1986-
Villanueva Cañas, José Luis, 1984-
Espinar, Lorena
Díez Antón, Juana, 1962-
Carey, Lucas, 1980-
Albà Soler, Mar
author_role author
author2 Ruiz Orera, Jorge, 1988-
Messeguer, Xavier
Blasco Moreno, Bernat, 1986-
Villanueva Cañas, José Luis, 1984-
Espinar, Lorena
Díez Antón, Juana, 1962-
Carey, Lucas, 1980-
Albà Soler, Mar
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Evolvability
Fungal genetics
Molecular evolution
Transcriptomics
topic Evolvability
Fungal genetics
Molecular evolution
Transcriptomics
description De novo gene origination has been recently established as an important mechanism for the formation of new genes. In organisms with a large genome, intergenic and intronic regions provide plenty of raw material for new transcriptional events to occur, but little is know about how de novo transcripts originate in more densely-packed genomes. Here, we identify 213 de novo originated transcripts in Saccharomyces cerevisiae using deep transcriptomics and genomic synteny information from multiple yeast species grown in two different conditions. We find that about half of the de novo transcripts are expressed from regions which already harbor other genes in the opposite orientation; these transcripts show similar expression changes in response to stress as their overlapping counterparts, and some appear to translate small proteins. Thus, a large fraction of de novo genes in yeast are likely to co-evolve with already existing genes.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/46484
http://dx.doi.org/10.1038/s41467-021-20911-3
url http://hdl.handle.net/10230/46484
http://dx.doi.org/10.1038/s41467-021-20911-3
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Nat Commun. 2021; 12(1):604
info:eu-repo/grantAgreement/ES/2PE/PGC2018-094091-B-I00
info:eu-repo/grantAgreement/ES/1PE/BFU2015-65235-P
info:eu-repo/grantAgreement/ES/1PE/BFU2015-68351-P
info:eu-repo/grantAgreement/ES/1PE/BFU2016-80039-R
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
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repository.mail.fl_str_mv
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