DataSheet_1_Expression of HMGCS2 in intestinal epithelial cells is downregulated in inflammatory bowel disease associated with endoplasmic reticulum stress.pdf [Dataset]

Martín-Adrados, Beatriz; Wculek, Stefanie K.; Fernández-Bravo, Sergio; Torres-Ruiz, Raúl; Gómez-Sánchez, María José; Hernández-Walias, José Carlos; Moraes Ferreira, Frederico; Corraliza, Ana María; Sancho, David; Esteban, Vanesa; Rodríguez-Perales, Sandra; Cruz-Adalia, Aránzazu; Nakaya, Helder I.; Salas, Azucena; Bernardo, David; Campos-Martín, Yolanda; Martínez-Zamorano, Elena; Muñoz-López, Diego; Gómez del Moral, Manuel; Cubero, Francisco Javier; Blumberg, Richard S.; Martínez-Naves, Eduardo

DataSheet_1_Expression of HMGCS2 in intestinal epithelial cells is downregulated in inflammatory bowel disease associated with endoplasmic reticulum stress.pdf [Dataset]

[Introduction]: The Unfolded Protein Response, a mechanism triggered by the cell in response to Endoplasmic reticulum stress, is linked to inflammatory responses. Our aim was to identify novel Unfolded Protein Response-mechanisms that might be involved in triggering or perpetuating the inflammatory...

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Authors:	Martín-Adrados, Beatriz, Wculek, Stefanie K., Fernández-Bravo, Sergio, Torres-Ruiz, Raúl, Gómez-Sánchez, María José, Hernández-Walias, José Carlos, Moraes Ferreira, Frederico, Corraliza, Ana María, Sancho, David, Esteban, Vanesa, Rodríguez-Perales, Sandra, Cruz-Adalia, Aránzazu, Nakaya, Helder I., Salas, Azucena, Bernardo, David, Campos-Martín, Yolanda, Martínez-Zamorano, Elena, Muñoz-López, Diego, Gómez del Moral, Manuel, Cubero, Francisco Javier, Blumberg, Richard S., Martínez-Naves, Eduardo
Format:	conjunto de datos
Status:	Published version
Publication Date:	2023
Country:	España
Institution:	Consejo Superior de Investigaciones Científicas (CSIC)
Repository:	DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:	oai:digital.csic.es:10261/360480
Online Access:	http://hdl.handle.net/10261/360480
Access Level:	Open access
Keyword:	Inflammatory bowel disease Inflammation ER stress HMGCS2 Unfolded protein response (UPR)

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spelling	DataSheet_1_Expression of HMGCS2 in intestinal epithelial cells is downregulated in inflammatory bowel disease associated with endoplasmic reticulum stress.pdf [Dataset]Martín-Adrados, BeatrizWculek, Stefanie K.Fernández-Bravo, SergioTorres-Ruiz, RaúlTorres-Ruiz, RaúlGómez-Sánchez, María JoséHernández-Walias, José CarlosMoraes Ferreira, FredericoCorraliza, Ana MaríaSancho, DavidEsteban, VanesaRodríguez-Perales, SandraCruz-Adalia, AránzazuNakaya, Helder I.Salas, AzucenaBernardo, DavidCampos-Martín, YolandaMartínez-Zamorano, ElenaMuñoz-López, DiegoGómez del Moral, ManuelCubero, Francisco JavierBlumberg, Richard S.Martínez-Naves, EduardoInflammatory bowel diseaseInflammationER stressHMGCS2Unfolded protein response (UPR)[Introduction]: The Unfolded Protein Response, a mechanism triggered by the cell in response to Endoplasmic reticulum stress, is linked to inflammatory responses. Our aim was to identify novel Unfolded Protein Response-mechanisms that might be involved in triggering or perpetuating the inflammatory response carried out by the Intestinal Epithelial Cells in the context of Inflammatory Bowel Disease.[Methods]: We analyzed the transcriptional profile of human Intestinal Epithelial Cell lines treated with an Endoplasmic Reticulum stress inducer (thapsigargin) and/or proinflammatory stimuli. Several genes were further analyzed in colonic biopsies from Ulcerative Colitis patients and healthy controls. Lastly, we generated Caco-2 cells lacking HMGCS2 by CRISPR Cas-9 and analyzed the functional implications of its absence in Intestinal Epithelial Cells.[Results]: Exposure to a TLR ligand after thapsigargin treatment resulted in a powerful synergistic modulation of gene expression, which led us to identify new genes and pathways that could be involved in inflammatory responses linked to the Unfolded Protein Response. Key differentially expressed genes in the array also exhibited transcriptional alterations in colonic biopsies from active Ulcerative Colitis patients, including NKG2D ligands and the enzyme HMGCS2. Moreover, functional studies showed altered metabolic responses and epithelial barrier integrity in HMGCS2 deficient cell lines.[Conclusion]: We have identified new genes and pathways that are regulated by the Unfolded Protein Response in the context of Inflammatory Bowel Disease including HMGCS2, a gene involved in the metabolism of Short Chain Fatty Acids that may have an important role in intestinal inflammation linked to Endoplasmic Reticulum stress and the resolution of the epithelial damage.Peer reviewedFrontiers MediaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/360480reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésMartín-Adrados, Beatriz; Wculek, Stefanie K.; Fernández-Bravo, Sergio; Torres-Ruiz, Raúl; Valle-Noguera, Ana; Gómez-Sánchez, María José; Hernández-Walias, José Carlos; Moraes Ferreira, Frederico; Corraliza, Ana María; Sancho, David; Esteban, Vanesa; Rodríguez-Perales, Sandra; Cruz-Adalia, Aránzazu; Nakaya, Helder I.; Salas, Azucena; Bernardo, David; Campos-Martín, Yolanda; Martínez-Zamorano, Elena; Muñoz-López, Diego; Gómez del Moral, Manuel; Cubero, Francisco Javier; Blumberg, Richard S.; Martínez-Naves, Eduardo. Expression of HMGCS2 in intestinal epithelial cells is downregulated in inflammatory bowel disease associated with endoplasmic reticulum stress. http://dx.doi.org/10.3389/fimmu.2023.1185517 . http://hdl.handle.net/10261/342560https://doi.org/10.3389/fimmu.2023.1185517.s001Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3604802026-05-22T06:33:51Z
dc.title.none.fl_str_mv	DataSheet_1_Expression of HMGCS2 in intestinal epithelial cells is downregulated in inflammatory bowel disease associated with endoplasmic reticulum stress.pdf [Dataset]
title	DataSheet_1_Expression of HMGCS2 in intestinal epithelial cells is downregulated in inflammatory bowel disease associated with endoplasmic reticulum stress.pdf [Dataset]
spellingShingle	DataSheet_1_Expression of HMGCS2 in intestinal epithelial cells is downregulated in inflammatory bowel disease associated with endoplasmic reticulum stress.pdf [Dataset] Martín-Adrados, Beatriz Inflammatory bowel disease Inflammation ER stress HMGCS2 Unfolded protein response (UPR)
title_short	DataSheet_1_Expression of HMGCS2 in intestinal epithelial cells is downregulated in inflammatory bowel disease associated with endoplasmic reticulum stress.pdf [Dataset]
title_full	DataSheet_1_Expression of HMGCS2 in intestinal epithelial cells is downregulated in inflammatory bowel disease associated with endoplasmic reticulum stress.pdf [Dataset]
title_fullStr	DataSheet_1_Expression of HMGCS2 in intestinal epithelial cells is downregulated in inflammatory bowel disease associated with endoplasmic reticulum stress.pdf [Dataset]
title_full_unstemmed	DataSheet_1_Expression of HMGCS2 in intestinal epithelial cells is downregulated in inflammatory bowel disease associated with endoplasmic reticulum stress.pdf [Dataset]
title_sort	DataSheet_1_Expression of HMGCS2 in intestinal epithelial cells is downregulated in inflammatory bowel disease associated with endoplasmic reticulum stress.pdf [Dataset]
dc.creator.none.fl_str_mv	Martín-Adrados, Beatriz Wculek, Stefanie K. Fernández-Bravo, Sergio Torres-Ruiz, Raúl Torres-Ruiz, Raúl Gómez-Sánchez, María José Hernández-Walias, José Carlos Moraes Ferreira, Frederico Corraliza, Ana María Sancho, David Esteban, Vanesa Rodríguez-Perales, Sandra Cruz-Adalia, Aránzazu Nakaya, Helder I. Salas, Azucena Bernardo, David Campos-Martín, Yolanda Martínez-Zamorano, Elena Muñoz-López, Diego Gómez del Moral, Manuel Cubero, Francisco Javier Blumberg, Richard S. Martínez-Naves, Eduardo
author	Martín-Adrados, Beatriz
author_facet	Martín-Adrados, Beatriz Wculek, Stefanie K. Fernández-Bravo, Sergio Torres-Ruiz, Raúl Gómez-Sánchez, María José Hernández-Walias, José Carlos Moraes Ferreira, Frederico Corraliza, Ana María Sancho, David Esteban, Vanesa Rodríguez-Perales, Sandra Cruz-Adalia, Aránzazu Nakaya, Helder I. Salas, Azucena Bernardo, David Campos-Martín, Yolanda Martínez-Zamorano, Elena Muñoz-López, Diego Gómez del Moral, Manuel Cubero, Francisco Javier Blumberg, Richard S. Martínez-Naves, Eduardo
author_role	author
author2	Wculek, Stefanie K. Fernández-Bravo, Sergio Torres-Ruiz, Raúl Gómez-Sánchez, María José Hernández-Walias, José Carlos Moraes Ferreira, Frederico Corraliza, Ana María Sancho, David Esteban, Vanesa Rodríguez-Perales, Sandra Cruz-Adalia, Aránzazu Nakaya, Helder I. Salas, Azucena Bernardo, David Campos-Martín, Yolanda Martínez-Zamorano, Elena Muñoz-López, Diego Gómez del Moral, Manuel Cubero, Francisco Javier Blumberg, Richard S. Martínez-Naves, Eduardo
author2_role	author author author author author author author author author author author author author author author author author author author author author
dc.contributor.none.fl_str_mv	Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv	Inflammatory bowel disease Inflammation ER stress HMGCS2 Unfolded protein response (UPR)
topic	Inflammatory bowel disease Inflammation ER stress HMGCS2 Unfolded protein response (UPR)
description	[Introduction]: The Unfolded Protein Response, a mechanism triggered by the cell in response to Endoplasmic reticulum stress, is linked to inflammatory responses. Our aim was to identify novel Unfolded Protein Response-mechanisms that might be involved in triggering or perpetuating the inflammatory response carried out by the Intestinal Epithelial Cells in the context of Inflammatory Bowel Disease.
publishDate	2023
dc.date.none.fl_str_mv	2023 2024 2024
dc.type.none.fl_str_mv	info:eu-repo/semantics/dataset http://purl.org/coar/resource_type/c_ddb1 Publisher's version info:eu-repo/semantics/publishedVersion
format	dataset
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/10261/360480
url	http://hdl.handle.net/10261/360480
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.relation.none.fl_str_mv	Martín-Adrados, Beatriz; Wculek, Stefanie K.; Fernández-Bravo, Sergio; Torres-Ruiz, Raúl; Valle-Noguera, Ana; Gómez-Sánchez, María José; Hernández-Walias, José Carlos; Moraes Ferreira, Frederico; Corraliza, Ana María; Sancho, David; Esteban, Vanesa; Rodríguez-Perales, Sandra; Cruz-Adalia, Aránzazu; Nakaya, Helder I.; Salas, Azucena; Bernardo, David; Campos-Martín, Yolanda; Martínez-Zamorano, Elena; Muñoz-López, Diego; Gómez del Moral, Manuel; Cubero, Francisco Javier; Blumberg, Richard S.; Martínez-Naves, Eduardo. Expression of HMGCS2 in intestinal epithelial cells is downregulated in inflammatory bowel disease associated with endoplasmic reticulum stress. http://dx.doi.org/10.3389/fimmu.2023.1185517 . http://hdl.handle.net/10261/342560 https://doi.org/10.3389/fimmu.2023.1185517.s001 Sí
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Frontiers Media
publisher.none.fl_str_mv	Frontiers Media
dc.source.none.fl_str_mv	reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str	Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str	DIGITAL.CSIC. Repositorio Institucional del CSIC
collection	DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,812455

DataSheet_1_Expression of HMGCS2 in intestinal epithelial cells is downregulated in inflammatory bowel disease associated with endoplasmic reticulum stress.pdf [Dataset]

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